Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.

<h4>Objective</h4>The current manuscript aims to determine the prevalence, duration and bacterial diversity of bacteraemia following dental extractions using conventional culture-dependent methods and 16S rDNA pyrosequencing.<h4>Methods</h4>The study group included 8 patients...

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Autores principales:	Alfonso Benítez-Páez, Maximiliano Álvarez, Pedro Belda-Ferre, Susana Rubido, Alex Mira, Inmaculada Tomás
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7641b043d71042e480458bcbddd5730a
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spelling	oai:doaj.org-article:7641b043d71042e480458bcbddd5730a2021-11-18T07:55:06ZDetection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.1932-620310.1371/journal.pone.0057782https://doaj.org/article/7641b043d71042e480458bcbddd5730a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23469240/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>The current manuscript aims to determine the prevalence, duration and bacterial diversity of bacteraemia following dental extractions using conventional culture-dependent methods and 16S rDNA pyrosequencing.<h4>Methods</h4>The study group included 8 patients undergoing dental extractions under general anaesthesia. Peripheral venous blood samples were collected at baseline, 30 seconds and 15 minutes after the dental extractions. Blood samples were analysed for bacteraemia applying conventional microbiological cultures under aerobic and anaerobic conditions as well as pyrosequencing using universal bacterial primers that target the 16S ribosomal DNA gene.<h4>Results</h4>Transient bacteremia was detected by culture-based methods in one sample at baseline time, in eight samples at 30 seconds, and in six samples at 15 minutes after surgical procedure; whereas bacteraemia was detected only in five blood samples at 30 seconds after dental extraction by using pyrosequencing. By applying conventional microbiological methods, a single microbial species was detected in six patients, and Streptococcus viridans was the most frequently cultured identified bacterium. By using pyrosequencing approaches however, the estimated blood microbial diversity after dental extractions was 13.4±1.7 bacterial families and 22.8±1.1 genera per sample.<h4>Conclusion</h4>The application of 16S rDNA pyrosequencing underestimated the prevalence and duration of bacteraemia following dental extractions, presumably due to not reaching the minimum DNA required for PCR amplification. However, this molecular technique, unlike conventional culture-dependent methods, revealed an extraordinarily high bacterial diversity of post-extraction bacteraemia. We propose that microorganisms recovered by culture may be only the tip of an iceberg of a really diverse microbiota whose viability and potential pathogenicity should be further studied.Alfonso Benítez-PáezMaximiliano ÁlvarezPedro Belda-FerreSusana RubidoAlex MiraInmaculada TomásPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57782 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alfonso Benítez-Páez Maximiliano Álvarez Pedro Belda-Ferre Susana Rubido Alex Mira Inmaculada Tomás Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
description	<h4>Objective</h4>The current manuscript aims to determine the prevalence, duration and bacterial diversity of bacteraemia following dental extractions using conventional culture-dependent methods and 16S rDNA pyrosequencing.<h4>Methods</h4>The study group included 8 patients undergoing dental extractions under general anaesthesia. Peripheral venous blood samples were collected at baseline, 30 seconds and 15 minutes after the dental extractions. Blood samples were analysed for bacteraemia applying conventional microbiological cultures under aerobic and anaerobic conditions as well as pyrosequencing using universal bacterial primers that target the 16S ribosomal DNA gene.<h4>Results</h4>Transient bacteremia was detected by culture-based methods in one sample at baseline time, in eight samples at 30 seconds, and in six samples at 15 minutes after surgical procedure; whereas bacteraemia was detected only in five blood samples at 30 seconds after dental extraction by using pyrosequencing. By applying conventional microbiological methods, a single microbial species was detected in six patients, and Streptococcus viridans was the most frequently cultured identified bacterium. By using pyrosequencing approaches however, the estimated blood microbial diversity after dental extractions was 13.4±1.7 bacterial families and 22.8±1.1 genera per sample.<h4>Conclusion</h4>The application of 16S rDNA pyrosequencing underestimated the prevalence and duration of bacteraemia following dental extractions, presumably due to not reaching the minimum DNA required for PCR amplification. However, this molecular technique, unlike conventional culture-dependent methods, revealed an extraordinarily high bacterial diversity of post-extraction bacteraemia. We propose that microorganisms recovered by culture may be only the tip of an iceberg of a really diverse microbiota whose viability and potential pathogenicity should be further studied.
format	article
author	Alfonso Benítez-Páez Maximiliano Álvarez Pedro Belda-Ferre Susana Rubido Alex Mira Inmaculada Tomás
author_facet	Alfonso Benítez-Páez Maximiliano Álvarez Pedro Belda-Ferre Susana Rubido Alex Mira Inmaculada Tomás
author_sort	Alfonso Benítez-Páez
title	Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
title_short	Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
title_full	Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
title_fullStr	Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
title_full_unstemmed	Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.
title_sort	detection of transient bacteraemia following dental extractions by 16s rdna pyrosequencing: a pilot study.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7641b043d71042e480458bcbddd5730a
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Detection of transient bacteraemia following dental extractions by 16S rDNA pyrosequencing: a pilot study.

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