Persistent mRNA and miRNA expression changes in irradiated baboons

Abstract We examined the transcriptome/post-transcriptome for persistent gene expression changes after radiation exposure in a baboon model. Eighteen baboons were irradiated with a whole body equivalent dose of 2.5 or 5 Gy. Blood samples were taken before, 7, 28 and 75–106 days after radiation expos...

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Autores principales:	Matthias Port, Francis Hérodin, Marco Valente, Michel Drouet, Patrick Ostheim, Matthäus Majewski, Michael Abend
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	miRNA Expression Changes Persistent Gene Expression Body Equivalent Dose Candidate mRNA Protein-coding mRNAs Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76425725cc2b439b8f9ce8cf0c447690
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spelling	oai:doaj.org-article:76425725cc2b439b8f9ce8cf0c4476902021-12-02T15:08:18ZPersistent mRNA and miRNA expression changes in irradiated baboons10.1038/s41598-018-33544-22045-2322https://doaj.org/article/76425725cc2b439b8f9ce8cf0c4476902018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33544-2https://doaj.org/toc/2045-2322Abstract We examined the transcriptome/post-transcriptome for persistent gene expression changes after radiation exposure in a baboon model. Eighteen baboons were irradiated with a whole body equivalent dose of 2.5 or 5 Gy. Blood samples were taken before, 7, 28 and 75–106 days after radiation exposure. Stage I was a whole genome screening for mRNA combined with a qRT-PCR platform for detection of 667 miRNAs. Candidate mRNAs and miRNAs differentially up- or down-regulated in stage I were chosen for validation in stage II using the remaining samples. Only 12 of 32 candidate genes provided analyzable results with two mRNAs showing significant 3–5-fold differences in gene expression over the reference (p < 0.0001). From 667 candidate miRNAs, 290 miRNA were eligible for analysis with 21 miRNAs independently validated using qRT-PCR. These miRNAs showed persistent expression changes on each day and over days 7–106 days after exposure (n = 7). In particular miR-212 involved in radiosensitivity and immune modulation appeared persistently and 48–77-fold up-regulated over the entire time period. We are finally trying to put our results into a context of clinical implications and provide possible hints on underlying molecular mechanisms to be examined in future studies.Matthias PortFrancis HérodinMarco ValenteMichel DrouetPatrick OstheimMatthäus MajewskiMichael AbendNature PortfolioarticlemiRNA Expression ChangesPersistent Gene ExpressionBody Equivalent DoseCandidate mRNAProtein-coding mRNAsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNA Expression Changes Persistent Gene Expression Body Equivalent Dose Candidate mRNA Protein-coding mRNAs Medicine R Science Q
spellingShingle	miRNA Expression Changes Persistent Gene Expression Body Equivalent Dose Candidate mRNA Protein-coding mRNAs Medicine R Science Q Matthias Port Francis Hérodin Marco Valente Michel Drouet Patrick Ostheim Matthäus Majewski Michael Abend Persistent mRNA and miRNA expression changes in irradiated baboons
description	Abstract We examined the transcriptome/post-transcriptome for persistent gene expression changes after radiation exposure in a baboon model. Eighteen baboons were irradiated with a whole body equivalent dose of 2.5 or 5 Gy. Blood samples were taken before, 7, 28 and 75–106 days after radiation exposure. Stage I was a whole genome screening for mRNA combined with a qRT-PCR platform for detection of 667 miRNAs. Candidate mRNAs and miRNAs differentially up- or down-regulated in stage I were chosen for validation in stage II using the remaining samples. Only 12 of 32 candidate genes provided analyzable results with two mRNAs showing significant 3–5-fold differences in gene expression over the reference (p < 0.0001). From 667 candidate miRNAs, 290 miRNA were eligible for analysis with 21 miRNAs independently validated using qRT-PCR. These miRNAs showed persistent expression changes on each day and over days 7–106 days after exposure (n = 7). In particular miR-212 involved in radiosensitivity and immune modulation appeared persistently and 48–77-fold up-regulated over the entire time period. We are finally trying to put our results into a context of clinical implications and provide possible hints on underlying molecular mechanisms to be examined in future studies.
format	article
author	Matthias Port Francis Hérodin Marco Valente Michel Drouet Patrick Ostheim Matthäus Majewski Michael Abend
author_facet	Matthias Port Francis Hérodin Marco Valente Michel Drouet Patrick Ostheim Matthäus Majewski Michael Abend
author_sort	Matthias Port
title	Persistent mRNA and miRNA expression changes in irradiated baboons
title_short	Persistent mRNA and miRNA expression changes in irradiated baboons
title_full	Persistent mRNA and miRNA expression changes in irradiated baboons
title_fullStr	Persistent mRNA and miRNA expression changes in irradiated baboons
title_full_unstemmed	Persistent mRNA and miRNA expression changes in irradiated baboons
title_sort	persistent mrna and mirna expression changes in irradiated baboons
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/76425725cc2b439b8f9ce8cf0c447690
work_keys_str_mv	AT matthiasport persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT francisherodin persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT marcovalente persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT micheldrouet persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT patrickostheim persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT matthausmajewski persistentmrnaandmirnaexpressionchangesinirradiatedbaboons AT michaelabend persistentmrnaandmirnaexpressionchangesinirradiatedbaboons
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Persistent mRNA and miRNA expression changes in irradiated baboons

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