Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231

Abstract Autophagy is one of the chemotherapy resistance mechanisms in breast cancer. The aim of this study was to determine the level of recruitment of the autophagy pathway in the triple-negative breast cancer (TNBC) cell line MDA-MB231 compared with that in the control luminal breast cancer cell...

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Autores principales: Christian Garbar, Corinne Mascaux, Jérôme Giustiniani, Yacine Merrouche, Armand Bensussan
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/76425cb34daa4467a38bdcbc1173f305
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spelling oai:doaj.org-article:76425cb34daa4467a38bdcbc1173f3052021-12-02T15:06:19ZChemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB23110.1038/s41598-017-07489-x2045-2322https://doaj.org/article/76425cb34daa4467a38bdcbc1173f3052017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07489-xhttps://doaj.org/toc/2045-2322Abstract Autophagy is one of the chemotherapy resistance mechanisms in breast cancer. The aim of this study was to determine the level of recruitment of the autophagy pathway in the triple-negative breast cancer (TNBC) cell line MDA-MB231 compared with that in the control luminal breast cancer cell line MCF7 before and after treatment with chemotherapy drugs. Furthermore, we investigated the relationship between autophagy and EGFR, MUC1 and IL17-receptors as activators of autophagy. Immunohistochemistry was performed in cell culture blocks using LC3b, MUC1-C, EGFR, IL17A, IL17-RA and IL17-RB antibodies. We found that the basal autophagy level in MDA-MB231 was high, whereas it was low in MCF7. However, in contrast to MDA-MB231, the autophagy level was increased in MCF7 upon treatment with chemotherapy agents. Interestingly, we observed that the expression levels of MUC1-C, EGFR, IL17-RA, and IL17-RB were not modified by the same treatments. Furthermore, the chemotherapy treatments did not increase autophagy in TNBC cells without affecting the expression levels of MUC1-C, EGFR, IL17-RA or IL17-RB.Christian GarbarCorinne MascauxJérôme GiustinianiYacine MerroucheArmand BensussanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Christian Garbar
Corinne Mascaux
Jérôme Giustiniani
Yacine Merrouche
Armand Bensussan
Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
description Abstract Autophagy is one of the chemotherapy resistance mechanisms in breast cancer. The aim of this study was to determine the level of recruitment of the autophagy pathway in the triple-negative breast cancer (TNBC) cell line MDA-MB231 compared with that in the control luminal breast cancer cell line MCF7 before and after treatment with chemotherapy drugs. Furthermore, we investigated the relationship between autophagy and EGFR, MUC1 and IL17-receptors as activators of autophagy. Immunohistochemistry was performed in cell culture blocks using LC3b, MUC1-C, EGFR, IL17A, IL17-RA and IL17-RB antibodies. We found that the basal autophagy level in MDA-MB231 was high, whereas it was low in MCF7. However, in contrast to MDA-MB231, the autophagy level was increased in MCF7 upon treatment with chemotherapy agents. Interestingly, we observed that the expression levels of MUC1-C, EGFR, IL17-RA, and IL17-RB were not modified by the same treatments. Furthermore, the chemotherapy treatments did not increase autophagy in TNBC cells without affecting the expression levels of MUC1-C, EGFR, IL17-RA or IL17-RB.
format article
author Christian Garbar
Corinne Mascaux
Jérôme Giustiniani
Yacine Merrouche
Armand Bensussan
author_facet Christian Garbar
Corinne Mascaux
Jérôme Giustiniani
Yacine Merrouche
Armand Bensussan
author_sort Christian Garbar
title Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
title_short Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
title_full Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
title_fullStr Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
title_full_unstemmed Chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell MCF7, but not in the triple-negative MDA-MB231
title_sort chemotherapy treatment induces an increase of autophagy in the luminal breast cancer cell mcf7, but not in the triple-negative mda-mb231
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/76425cb34daa4467a38bdcbc1173f305
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AT jeromegiustiniani chemotherapytreatmentinducesanincreaseofautophagyintheluminalbreastcancercellmcf7butnotinthetriplenegativemdamb231
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