Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway

Xiulong Li,1 Hongfeng Wang2 1Department of Neurosurgery, Caoxian People’s Hospital, Heze, 274400, Shandong, People’s Republic of China; 2Department of Neurosurgery, Yantai Harbour Hospital, Yantai, 264000, Shandong, People’s Republic of ChinaCorrespondence: Hongfeng Wan...

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Autores principales:	Li X, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	glioma lncrna gabpb1-as1 mir-330 znf367 cell cycle signaling pathway Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/764447cfa24e4a9d979df31d8aad0fd7
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spelling	oai:doaj.org-article:764447cfa24e4a9d979df31d8aad0fd72021-12-02T15:23:24ZLong Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway1178-2021https://doaj.org/article/764447cfa24e4a9d979df31d8aad0fd72021-07-01T00:00:00Zhttps://www.dovepress.com/long-non-coding-rna-gabpb1-as1-augments-malignancy-of-glioma-cells-by--peer-reviewed-fulltext-article-NDThttps://doaj.org/toc/1178-2021Xiulong Li,1 Hongfeng Wang2 1Department of Neurosurgery, Caoxian People’s Hospital, Heze, 274400, Shandong, People’s Republic of China; 2Department of Neurosurgery, Yantai Harbour Hospital, Yantai, 264000, Shandong, People’s Republic of ChinaCorrespondence: Hongfeng WangDepartment of Neurosurgery, Yantai Harbour Hospital, No. 100, Xingfu Road, Zhifu District, Yantai, 264000, Shandong, People’s Republic of ChinaTel/Fax +86-15762793079Email hfWang_9101@126.comBackground: Deregulation of long non-coding RNAs (lncRNAs) is frequently relevant to the malignant phenotypical changes. This study aimed to explore the role of lncRNA GABPB1-AS1 in the malignancy of glioma cells.Methods: Abnormally expressed genes in glioma were analyzed using a GEO GSE2223 dataset. Short hairpin (sh) RNA silencing of GABPB1-AS1 was introduced in glioma cells to explore its correlation with the proliferation, apoptosis, and invasiveness of cancer cells. The target transcripts of GABPB1-AS1 were predicted by bioinformatics analyses. MicroRNA (miR)-330 inhibition was additionally introduced in the glioma cells after GABPB1-AS1 knockdown for rescue experiments. Animal studies were performed by inducing xenograft tumors in nude mice.Results: GABPB1-AS1 was highly expressed in the glioma tissues and associated with advanced WHO grades. GABPB1-AS1 knockdown reduced proliferation and invasiveness of glioma cells in vitro and in vivo. miR-330 was a target transcript of GABPB1-AS1. miR-330 inhibition restored the malignancy of glioma cells. miR-330 directly bound to ZNF367. ZNF367 was highly expressed in glioma tissues and positively correlated with GABPB1-AS1 expression, and it was relevant to the cell cycle signaling pathway. Downregulation of GABPB1-AS1 reduced the expression of ZNF367 and reduced the levels of cell cycle-related proteins PCNA, CDC20, CDC7 and CCNA1 in cells.Conclusion: This study demonstrated that GABPB1-AS1 competitively bound to miR-330 and de-repressed ZNF367 expression, leading to activation of the cell cycle signaling pathway and the growth and metastasis of glioma cells.Keywords: glioma, lncRNA GABPB1-AS1, miR-330, ZNF367, cell cycle signaling pathwayLi XWang HDove Medical Pressarticlegliomalncrna gabpb1-as1mir-330znf367cell cycle signaling pathwayNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 2073-2087 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma lncrna gabpb1-as1 mir-330 znf367 cell cycle signaling pathway Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	glioma lncrna gabpb1-as1 mir-330 znf367 cell cycle signaling pathway Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Li X Wang H Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
description	Xiulong Li,1 Hongfeng Wang2 1Department of Neurosurgery, Caoxian People’s Hospital, Heze, 274400, Shandong, People’s Republic of China; 2Department of Neurosurgery, Yantai Harbour Hospital, Yantai, 264000, Shandong, People’s Republic of ChinaCorrespondence: Hongfeng WangDepartment of Neurosurgery, Yantai Harbour Hospital, No. 100, Xingfu Road, Zhifu District, Yantai, 264000, Shandong, People’s Republic of ChinaTel/Fax +86-15762793079Email hfWang_9101@126.comBackground: Deregulation of long non-coding RNAs (lncRNAs) is frequently relevant to the malignant phenotypical changes. This study aimed to explore the role of lncRNA GABPB1-AS1 in the malignancy of glioma cells.Methods: Abnormally expressed genes in glioma were analyzed using a GEO GSE2223 dataset. Short hairpin (sh) RNA silencing of GABPB1-AS1 was introduced in glioma cells to explore its correlation with the proliferation, apoptosis, and invasiveness of cancer cells. The target transcripts of GABPB1-AS1 were predicted by bioinformatics analyses. MicroRNA (miR)-330 inhibition was additionally introduced in the glioma cells after GABPB1-AS1 knockdown for rescue experiments. Animal studies were performed by inducing xenograft tumors in nude mice.Results: GABPB1-AS1 was highly expressed in the glioma tissues and associated with advanced WHO grades. GABPB1-AS1 knockdown reduced proliferation and invasiveness of glioma cells in vitro and in vivo. miR-330 was a target transcript of GABPB1-AS1. miR-330 inhibition restored the malignancy of glioma cells. miR-330 directly bound to ZNF367. ZNF367 was highly expressed in glioma tissues and positively correlated with GABPB1-AS1 expression, and it was relevant to the cell cycle signaling pathway. Downregulation of GABPB1-AS1 reduced the expression of ZNF367 and reduced the levels of cell cycle-related proteins PCNA, CDC20, CDC7 and CCNA1 in cells.Conclusion: This study demonstrated that GABPB1-AS1 competitively bound to miR-330 and de-repressed ZNF367 expression, leading to activation of the cell cycle signaling pathway and the growth and metastasis of glioma cells.Keywords: glioma, lncRNA GABPB1-AS1, miR-330, ZNF367, cell cycle signaling pathway
format	article
author	Li X Wang H
author_facet	Li X Wang H
author_sort	Li X
title	Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
title_short	Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
title_full	Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
title_fullStr	Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
title_full_unstemmed	Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway
title_sort	long non-coding rna gabpb1-as1 augments malignancy of glioma cells by sequestering microrna-330 and reinforcing the znf367/cell cycle signaling pathway
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/764447cfa24e4a9d979df31d8aad0fd7
work_keys_str_mv	AT lix longnoncodingrnagabpb1as1augmentsmalignancyofgliomacellsbysequesteringmicrorna330andreinforcingtheznf367cellcyclesignalingpathway AT wangh longnoncodingrnagabpb1as1augmentsmalignancyofgliomacellsbysequesteringmicrorna330andreinforcingtheznf367cellcyclesignalingpathway
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Long Non-Coding RNA GABPB1-AS1 Augments Malignancy of Glioma Cells by Sequestering MicroRNA-330 and Reinforcing the ZNF367/Cell Cycle Signaling Pathway

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