Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.

Degeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Claire Cunningham, Akshay Srivastava, Estelle Collin, Sibylle Grad, Mauro Alini, Abhay Pandit, J Gerard Wall
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7649b23856784f408908e5b98f0516ca
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7649b23856784f408908e5b98f0516ca
record_format	dspace
spelling	oai:doaj.org-article:7649b23856784f408908e5b98f0516ca2021-11-18T08:41:56ZIsolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.1932-620310.1371/journal.pone.0083678https://doaj.org/article/7649b23856784f408908e5b98f0516ca2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349544/?tool=EBIhttps://doaj.org/toc/1932-6203Degeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic factors. Cell-targeting strategies are likely to improve delivery due to the low cell numbers in the IVD. Single-chain antibody fragments (scFvs) that bind IVD cells were isolated for potential delivery of therapeutics to degenerated IVD. The most cell-distal domain of neural cell adhesion molecule 1 (NCAM1) was cloned and expressed in Escherichia coli. Phage display technology was used to isolate a human scFv against the recombinant domain by panning a scFv library on the immobilised protein. The isolated scFv bound cultured rat astrocytes, as well as bovine nucleus pulposus and annulus fibrosus cells in immunocytochemical studies. The scFv also labelled cells in bovine spinal cord and six-month and two-year old bovine IVD sections by immunohistochemistry. Antibody fragments can provide cell-binding moieties at improved cost, time, yield and functionalisation potential over whole antibodies. The described scFv has potential application in delivery of therapeutics to NCAM1-expressing cells in degenerated IVD.Claire CunninghamAkshay SrivastavaEstelle CollinSibylle GradMauro AliniAbhay PanditJ Gerard WallPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83678 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire Cunningham Akshay Srivastava Estelle Collin Sibylle Grad Mauro Alini Abhay Pandit J Gerard Wall Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
description	Degeneration of the intervertebral discs (IVD) is a leading cause of neck and low back pain. Degeneration begins in the central nucleus pulposus region, leading to loss of IVD osmotic properties. Regeneration approaches include administration of matrix-mimicking scaffolds, cells and/or therapeutic factors. Cell-targeting strategies are likely to improve delivery due to the low cell numbers in the IVD. Single-chain antibody fragments (scFvs) that bind IVD cells were isolated for potential delivery of therapeutics to degenerated IVD. The most cell-distal domain of neural cell adhesion molecule 1 (NCAM1) was cloned and expressed in Escherichia coli. Phage display technology was used to isolate a human scFv against the recombinant domain by panning a scFv library on the immobilised protein. The isolated scFv bound cultured rat astrocytes, as well as bovine nucleus pulposus and annulus fibrosus cells in immunocytochemical studies. The scFv also labelled cells in bovine spinal cord and six-month and two-year old bovine IVD sections by immunohistochemistry. Antibody fragments can provide cell-binding moieties at improved cost, time, yield and functionalisation potential over whole antibodies. The described scFv has potential application in delivery of therapeutics to NCAM1-expressing cells in degenerated IVD.
format	article
author	Claire Cunningham Akshay Srivastava Estelle Collin Sibylle Grad Mauro Alini Abhay Pandit J Gerard Wall
author_facet	Claire Cunningham Akshay Srivastava Estelle Collin Sibylle Grad Mauro Alini Abhay Pandit J Gerard Wall
author_sort	Claire Cunningham
title	Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
title_short	Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
title_full	Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
title_fullStr	Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
title_full_unstemmed	Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.
title_sort	isolation and characterisation of a recombinant antibody fragment that binds ncam1-expressing intervertebral disc cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7649b23856784f408908e5b98f0516ca
work_keys_str_mv	AT clairecunningham isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT akshaysrivastava isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT estellecollin isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT sibyllegrad isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT mauroalini isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT abhaypandit isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells AT jgerardwall isolationandcharacterisationofarecombinantantibodyfragmentthatbindsncam1expressingintervertebraldisccells
_version_	1718421452707856384

Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells.

Ejemplares similares