Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs

African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected wi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ann Sofie Olesen, Miyako Kodama, Louise Lohse, Francesc Accensi, Thomas Bruun Rasmussen, Christina M. Lazov, Morten T. Limborg, M. Thomas P. Gilbert, Anette Bøtner, Graham J. Belsham
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	African swine fever virus transcriptomics gene expression PBMCs RNAseq Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/764a825623974a19a4c325897d4448f0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:764a825623974a19a4c325897d4448f0
record_format	dspace
spelling	oai:doaj.org-article:764a825623974a19a4c325897d4448f02021-11-25T19:14:40ZIdentification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs10.3390/v131123331999-4915https://doaj.org/article/764a825623974a19a4c325897d4448f02021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2333https://doaj.org/toc/1999-4915African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.Ann Sofie OlesenMiyako KodamaLouise LohseFrancesc AccensiThomas Bruun RasmussenChristina M. LazovMorten T. LimborgM. Thomas P. GilbertAnette BøtnerGraham J. BelshamMDPI AGarticleAfrican swine fever virustranscriptomicsgene expressionPBMCsRNAseqMicrobiologyQR1-502ENViruses, Vol 13, Iss 2333, p 2333 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	African swine fever virus transcriptomics gene expression PBMCs RNAseq Microbiology QR1-502
spellingShingle	African swine fever virus transcriptomics gene expression PBMCs RNAseq Microbiology QR1-502 Ann Sofie Olesen Miyako Kodama Louise Lohse Francesc Accensi Thomas Bruun Rasmussen Christina M. Lazov Morten T. Limborg M. Thomas P. Gilbert Anette Bøtner Graham J. Belsham Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
description	African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.
format	article
author	Ann Sofie Olesen Miyako Kodama Louise Lohse Francesc Accensi Thomas Bruun Rasmussen Christina M. Lazov Morten T. Limborg M. Thomas P. Gilbert Anette Bøtner Graham J. Belsham
author_facet	Ann Sofie Olesen Miyako Kodama Louise Lohse Francesc Accensi Thomas Bruun Rasmussen Christina M. Lazov Morten T. Limborg M. Thomas P. Gilbert Anette Bøtner Graham J. Belsham
author_sort	Ann Sofie Olesen
title	Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
title_short	Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
title_full	Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
title_fullStr	Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
title_full_unstemmed	Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
title_sort	identification of african swine fever virus transcription within peripheral blood mononuclear cells of acutely infected pigs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/764a825623974a19a4c325897d4448f0
work_keys_str_mv	AT annsofieolesen identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT miyakokodama identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT louiselohse identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT francescaccensi identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT thomasbruunrasmussen identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT christinamlazov identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT mortentlimborg identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT mthomaspgilbert identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT anettebøtner identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs AT grahamjbelsham identificationofafricanswinefevervirustranscriptionwithinperipheralbloodmononuclearcellsofacutelyinfectedpigs
_version_	1718410098211028992

Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs

Ejemplares similares