Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02
Abstract Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assemb...
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2017
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oai:doaj.org-article:765625209068484ebcc991445d23457d2021-12-02T12:30:43ZComparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A0210.1038/s41598-017-09532-32045-2322https://doaj.org/article/765625209068484ebcc991445d23457d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09532-3https://doaj.org/toc/2045-2322Abstract Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molecule real-time (SMRT) sequencing. The assembled genome comprises a 9,307,519-bp linear chromosome with a GC content of 70.67%, and contained 8,888 predicted genes. Comparative genomics and natamycin biosynthetic gene cluster (BGC) analysis showed that BGC are highly conserved among evolutionarily diverse strains, and they also shared closer genome evolution compared with other Streptomyces species. Forty gene clusters were predicted to involve in the secondary metabolism of A02, and it was richly displayed in two-component signal transduction systems (TCS) in the genome, indicating a complex regulatory systems and high diversity of metabolites. Disruption of the phoP gene of the phoR-phoP TCS and nsdA gene confirmed phosphate sensitivity and global negative regulation of natamycin production. The genome sequence and analyses presented in this study provide an important molecular basis for research on natamycin production in Streptomyces, which could facilitate rational genome modification to improve the industrial use of A02.Huiling WuWeicheng LiuLingling ShiKaiwei SiTing LiuDan DongTaotao ZhangJuan ZhaoDewen LiuZhaofeng TianYuesen YueHong ZhangBai XuelianYong LiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Huiling Wu Weicheng Liu Lingling Shi Kaiwei Si Ting Liu Dan Dong Taotao Zhang Juan Zhao Dewen Liu Zhaofeng Tian Yuesen Yue Hong Zhang Bai Xuelian Yong Liang Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| description |
Abstract Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molecule real-time (SMRT) sequencing. The assembled genome comprises a 9,307,519-bp linear chromosome with a GC content of 70.67%, and contained 8,888 predicted genes. Comparative genomics and natamycin biosynthetic gene cluster (BGC) analysis showed that BGC are highly conserved among evolutionarily diverse strains, and they also shared closer genome evolution compared with other Streptomyces species. Forty gene clusters were predicted to involve in the secondary metabolism of A02, and it was richly displayed in two-component signal transduction systems (TCS) in the genome, indicating a complex regulatory systems and high diversity of metabolites. Disruption of the phoP gene of the phoR-phoP TCS and nsdA gene confirmed phosphate sensitivity and global negative regulation of natamycin production. The genome sequence and analyses presented in this study provide an important molecular basis for research on natamycin production in Streptomyces, which could facilitate rational genome modification to improve the industrial use of A02. |
| format |
article |
| author |
Huiling Wu Weicheng Liu Lingling Shi Kaiwei Si Ting Liu Dan Dong Taotao Zhang Juan Zhao Dewen Liu Zhaofeng Tian Yuesen Yue Hong Zhang Bai Xuelian Yong Liang |
| author_facet |
Huiling Wu Weicheng Liu Lingling Shi Kaiwei Si Ting Liu Dan Dong Taotao Zhang Juan Zhao Dewen Liu Zhaofeng Tian Yuesen Yue Hong Zhang Bai Xuelian Yong Liang |
| author_sort |
Huiling Wu |
| title |
Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| title_short |
Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| title_full |
Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| title_fullStr |
Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| title_full_unstemmed |
Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02 |
| title_sort |
comparative genomic and regulatory analyses of natamycin production of streptomyces lydicus a02 |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/765625209068484ebcc991445d23457d |
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