Transportation protocols for accurate assessment of microbial burden classification using molecular methods

Abstract Point-of-care testing is cost-effective, rapid, and could assist in avoiding hospital visits during a pandemic. However, they present some significant risks that current technologies cannot fully address. Skin flora contamination and insufficient specimen volume are two major limitations pr...

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Autores principales:	Amelia Kung, Jade Chen, Michael Tomasek, Dakai Liu, William Rodgers, Vincent Gau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7668db29979d4c1299ba563981e4cb3e
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spelling	oai:doaj.org-article:7668db29979d4c1299ba563981e4cb3e2021-12-02T16:27:50ZTransportation protocols for accurate assessment of microbial burden classification using molecular methods10.1038/s41598-021-95619-x2045-2322https://doaj.org/article/7668db29979d4c1299ba563981e4cb3e2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95619-xhttps://doaj.org/toc/2045-2322Abstract Point-of-care testing is cost-effective, rapid, and could assist in avoiding hospital visits during a pandemic. However, they present some significant risks that current technologies cannot fully address. Skin flora contamination and insufficient specimen volume are two major limitations preventing self-collection microbiological testing outside of hospital settings. We are developing a hybrid testing procedure to bridge the laboratory test with patient-side specimen collection and transportation for molecular microbial classification of causative bacterial infection and early identification of microbial susceptibility profiles directly from whole blood or urine specimens collected patient-side by health care workers such as phlebotomists in nursing homes or family clinics. This feasibility study presents our initial development efforts, in which we tested various transportation conditions (tubes, temperature, duration) for direct-from-specimen viable pathogen detection to determine the ideal conditions that allowed for differentiation between contaminant and causative bacteria in urine specimens and optimal growth for low-concentration blood specimens after transportation. For direct-from-urine assays, the viable pathogen at the clinical cutoff of 105 CFU/mL was detected after transportation with molecular assays while contaminants (≤ 104 CFU/mL) were not. For direct-from-blood assays, contrived blood samples as low as 0.8 CFU/mL were reported positive after transportation without the need for blood culture.Amelia KungJade ChenMichael TomasekDakai LiuWilliam RodgersVincent GauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amelia Kung Jade Chen Michael Tomasek Dakai Liu William Rodgers Vincent Gau Transportation protocols for accurate assessment of microbial burden classification using molecular methods
description	Abstract Point-of-care testing is cost-effective, rapid, and could assist in avoiding hospital visits during a pandemic. However, they present some significant risks that current technologies cannot fully address. Skin flora contamination and insufficient specimen volume are two major limitations preventing self-collection microbiological testing outside of hospital settings. We are developing a hybrid testing procedure to bridge the laboratory test with patient-side specimen collection and transportation for molecular microbial classification of causative bacterial infection and early identification of microbial susceptibility profiles directly from whole blood or urine specimens collected patient-side by health care workers such as phlebotomists in nursing homes or family clinics. This feasibility study presents our initial development efforts, in which we tested various transportation conditions (tubes, temperature, duration) for direct-from-specimen viable pathogen detection to determine the ideal conditions that allowed for differentiation between contaminant and causative bacteria in urine specimens and optimal growth for low-concentration blood specimens after transportation. For direct-from-urine assays, the viable pathogen at the clinical cutoff of 105 CFU/mL was detected after transportation with molecular assays while contaminants (≤ 104 CFU/mL) were not. For direct-from-blood assays, contrived blood samples as low as 0.8 CFU/mL were reported positive after transportation without the need for blood culture.
format	article
author	Amelia Kung Jade Chen Michael Tomasek Dakai Liu William Rodgers Vincent Gau
author_facet	Amelia Kung Jade Chen Michael Tomasek Dakai Liu William Rodgers Vincent Gau
author_sort	Amelia Kung
title	Transportation protocols for accurate assessment of microbial burden classification using molecular methods
title_short	Transportation protocols for accurate assessment of microbial burden classification using molecular methods
title_full	Transportation protocols for accurate assessment of microbial burden classification using molecular methods
title_fullStr	Transportation protocols for accurate assessment of microbial burden classification using molecular methods
title_full_unstemmed	Transportation protocols for accurate assessment of microbial burden classification using molecular methods
title_sort	transportation protocols for accurate assessment of microbial burden classification using molecular methods
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7668db29979d4c1299ba563981e4cb3e
work_keys_str_mv	AT ameliakung transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods AT jadechen transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods AT michaeltomasek transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods AT dakailiu transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods AT williamrodgers transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods AT vincentgau transportationprotocolsforaccurateassessmentofmicrobialburdenclassificationusingmolecularmethods
_version_	1718383998049189888

Transportation protocols for accurate assessment of microbial burden classification using molecular methods

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