Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone

In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties synthesized by the seed-mediated method, the...

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Autores principales:	Tianyu Ma, Kaixin Liu, Xiao Yang, Jingying Yang, Mingfei Pan, Shuo Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zearalenone gold nanorods indirect competitive ELISA visualized multicolor ELISA Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/767786a00e0f46d3bc3c2a32dae9bd8f
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spelling	oai:doaj.org-article:767786a00e0f46d3bc3c2a32dae9bd8f2021-11-25T17:33:52ZDevelopment of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone10.3390/foods101126542304-8158https://doaj.org/article/767786a00e0f46d3bc3c2a32dae9bd8f2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2654https://doaj.org/toc/2304-8158In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties synthesized by the seed-mediated method, the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and the AuNRs growth-based multicolor ELISA for detecting ZEN toxin were further established. Under the optimal experimental conditions, the coating amount of ZEN-OVA: 0.025 μg/well, antibody (Ab) dilution factor: 32,000 times, blocking solution: 0.5% skimmed milk powder, enzyme-labeled secondary Ab diluted 10,000 times, and a pH of the PBS buffer at 7.4, the sensitivity (<i>IC</i><sub>50</sub>) of the established ic-ELISA for ZEN detection reached 0.85 ± 0.04 μg/L, and the limit of detection (<i>IC</i><sub>15</sub>) reached 0.22 ± 0.08 μg/L. In the multicolor ELISA based on the growth of AuNRs, as the content of ZEN increased, the mixed solution exhibited a significant color change from brownish red to colorless. ZEN concentration as low as 0.1 μg/L can be detected by the naked eye (brown red to dark gray). This study provided an effective analysis strategy for the rapid screening and accurate monitoring of the ZEN contaminant in foods.Tianyu MaKaixin LiuXiao YangJingying YangMingfei PanShuo WangMDPI AGarticlezearalenonegold nanorodsindirect competitive ELISAvisualized multicolor ELISAChemical technologyTP1-1185ENFoods, Vol 10, Iss 2654, p 2654 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zearalenone gold nanorods indirect competitive ELISA visualized multicolor ELISA Chemical technology TP1-1185
spellingShingle	zearalenone gold nanorods indirect competitive ELISA visualized multicolor ELISA Chemical technology TP1-1185 Tianyu Ma Kaixin Liu Xiao Yang Jingying Yang Mingfei Pan Shuo Wang Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
description	In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties synthesized by the seed-mediated method, the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and the AuNRs growth-based multicolor ELISA for detecting ZEN toxin were further established. Under the optimal experimental conditions, the coating amount of ZEN-OVA: 0.025 μg/well, antibody (Ab) dilution factor: 32,000 times, blocking solution: 0.5% skimmed milk powder, enzyme-labeled secondary Ab diluted 10,000 times, and a pH of the PBS buffer at 7.4, the sensitivity (<i>IC</i><sub>50</sub>) of the established ic-ELISA for ZEN detection reached 0.85 ± 0.04 μg/L, and the limit of detection (<i>IC</i><sub>15</sub>) reached 0.22 ± 0.08 μg/L. In the multicolor ELISA based on the growth of AuNRs, as the content of ZEN increased, the mixed solution exhibited a significant color change from brownish red to colorless. ZEN concentration as low as 0.1 μg/L can be detected by the naked eye (brown red to dark gray). This study provided an effective analysis strategy for the rapid screening and accurate monitoring of the ZEN contaminant in foods.
format	article
author	Tianyu Ma Kaixin Liu Xiao Yang Jingying Yang Mingfei Pan Shuo Wang
author_facet	Tianyu Ma Kaixin Liu Xiao Yang Jingying Yang Mingfei Pan Shuo Wang
author_sort	Tianyu Ma
title	Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
title_short	Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
title_full	Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
title_fullStr	Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
title_full_unstemmed	Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone
title_sort	development of indirect competitive elisa and visualized multicolor elisa based on gold nanorods growth for the determination of zearalenone
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/767786a00e0f46d3bc3c2a32dae9bd8f
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_version_	1718412230991544320

Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone

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