Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells

Simultaneous quantification of DNA, RNA and protein at the single cell level has not yet been possible. Here the authors introduce a molecular labelling and detection strategy to quantify synthesis of these biomolecules and couple it to transient cell states through parallel quantification of state-...

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Autores principales: Samuel C. Kimmey, Luciene Borges, Reema Baskar, Sean C. Bendall
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/767c29a6bd7f4d73a67d7b29da9a2d79
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Sumario:Simultaneous quantification of DNA, RNA and protein at the single cell level has not yet been possible. Here the authors introduce a molecular labelling and detection strategy to quantify synthesis of these biomolecules and couple it to transient cell states through parallel quantification of state-dependent biomolecules.