The influence of storage time on ponazuril concentrations in feline plasma

Background The pharmacokinetics of ponazuril have been determined in several species; however, there is very little information on the stability of the drug after storage for long periods of time. This study was undertaken to determine the stability of ponazuril in plasma samples stored at −80 °C, w...

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Autores principales:	Sherry Cox, Lainey Harvill, Sarah Singleton, Joan B. Bergman, Becky DeBolt
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Ponazuril Stability Storage Plasma Medicine R
Acceso en línea:	https://doaj.org/article/7682f40b62c04c4fbd989236b63bef9d
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spelling	oai:doaj.org-article:7682f40b62c04c4fbd989236b63bef9d2021-11-28T15:05:16ZThe influence of storage time on ponazuril concentrations in feline plasma10.7717/peerj.125162167-8359https://doaj.org/article/7682f40b62c04c4fbd989236b63bef9d2021-11-01T00:00:00Zhttps://peerj.com/articles/12516.pdfhttps://peerj.com/articles/12516/https://doaj.org/toc/2167-8359Background The pharmacokinetics of ponazuril have been determined in several species; however, there is very little information on the stability of the drug after storage for long periods of time. This study was undertaken to determine the stability of ponazuril in plasma samples stored at −80 °C, which is the temperature most commonly used in the author’s laboratory. Method Spiked plasma samples (0.3, 7.5, and 15 µg/mL) were stored at −80 °C for three months. Analysis occurred on the first day and then once a week for the following twelve weeks. The drug was extracted using a chloroform extraction and separated by high performance liquid chromatography using ultraviolet detection. Results There was no loss of drug for any concentration for the first four weeks of storage. There was an average loss of less than 5% from day 35 through day 70 and an average loss of 6% on day 77 and 84. The data suggest that ponazuril is stable for 4 weeks when stored at −80 °C and undergoes minimal loss in the remaining 8 weeks.Sherry CoxLainey HarvillSarah SingletonJoan B. BergmanBecky DeBoltPeerJ Inc.articlePonazurilStabilityStoragePlasmaMedicineRENPeerJ, Vol 9, p e12516 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ponazuril Stability Storage Plasma Medicine R
spellingShingle	Ponazuril Stability Storage Plasma Medicine R Sherry Cox Lainey Harvill Sarah Singleton Joan B. Bergman Becky DeBolt The influence of storage time on ponazuril concentrations in feline plasma
description	Background The pharmacokinetics of ponazuril have been determined in several species; however, there is very little information on the stability of the drug after storage for long periods of time. This study was undertaken to determine the stability of ponazuril in plasma samples stored at −80 °C, which is the temperature most commonly used in the author’s laboratory. Method Spiked plasma samples (0.3, 7.5, and 15 µg/mL) were stored at −80 °C for three months. Analysis occurred on the first day and then once a week for the following twelve weeks. The drug was extracted using a chloroform extraction and separated by high performance liquid chromatography using ultraviolet detection. Results There was no loss of drug for any concentration for the first four weeks of storage. There was an average loss of less than 5% from day 35 through day 70 and an average loss of 6% on day 77 and 84. The data suggest that ponazuril is stable for 4 weeks when stored at −80 °C and undergoes minimal loss in the remaining 8 weeks.
format	article
author	Sherry Cox Lainey Harvill Sarah Singleton Joan B. Bergman Becky DeBolt
author_facet	Sherry Cox Lainey Harvill Sarah Singleton Joan B. Bergman Becky DeBolt
author_sort	Sherry Cox
title	The influence of storage time on ponazuril concentrations in feline plasma
title_short	The influence of storage time on ponazuril concentrations in feline plasma
title_full	The influence of storage time on ponazuril concentrations in feline plasma
title_fullStr	The influence of storage time on ponazuril concentrations in feline plasma
title_full_unstemmed	The influence of storage time on ponazuril concentrations in feline plasma
title_sort	influence of storage time on ponazuril concentrations in feline plasma
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/7682f40b62c04c4fbd989236b63bef9d
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The influence of storage time on ponazuril concentrations in feline plasma

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