TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.

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Detalles Bibliográficos
Autores principales:	Ángel J. Picher, Bettina Budeus, Oliver Wafzig, Carola Krüger, Sara García-Gómez, María I. Martínez-Jiménez, Alberto Díaz-Talavera, Daniela Weber, Luis Blanco, Armin Schneider
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7684ce22eed44d1aa7aa7d8f45a853bc
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spelling	oai:doaj.org-article:7684ce22eed44d1aa7aa7d8f45a853bc2021-12-02T16:57:18ZTruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol10.1038/ncomms132962041-1723https://doaj.org/article/7684ce22eed44d1aa7aa7d8f45a853bc2016-11-01T00:00:00Zhttps://doi.org/10.1038/ncomms13296https://doaj.org/toc/2041-1723Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.Ángel J. PicherBettina BudeusOliver WafzigCarola KrügerSara García-GómezMaría I. Martínez-JiménezAlberto Díaz-TalaveraDaniela WeberLuis BlancoArmin SchneiderNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-16 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ángel J. Picher Bettina Budeus Oliver Wafzig Carola Krüger Sara García-Gómez María I. Martínez-Jiménez Alberto Díaz-Talavera Daniela Weber Luis Blanco Armin Schneider TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
description	Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.
format	article
author	Ángel J. Picher Bettina Budeus Oliver Wafzig Carola Krüger Sara García-Gómez María I. Martínez-Jiménez Alberto Díaz-Talavera Daniela Weber Luis Blanco Armin Schneider
author_facet	Ángel J. Picher Bettina Budeus Oliver Wafzig Carola Krüger Sara García-Gómez María I. Martínez-Jiménez Alberto Díaz-Talavera Daniela Weber Luis Blanco Armin Schneider
author_sort	Ángel J. Picher
title	TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
title_short	TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
title_full	TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
title_fullStr	TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
title_full_unstemmed	TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol
title_sort	trueprime is a novel method for whole-genome amplification from single cells based on tthprimpol
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/7684ce22eed44d1aa7aa7d8f45a853bc
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TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

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