TruePrime is a novel method for whole-genome amplification from single cells based on TthPrimPol

Single cell genomic analysis needs DNA amplification with high fidelity and accuracy. Here, the authors devise a novel multiple displacement amplification method called TruePrime that is based in Thermus thermophilusPrimPol and Phi29 DNA polymerase, and demonstrate its utility and accuracy.

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Detalles Bibliográficos
Autores principales: Ángel J. Picher, Bettina Budeus, Oliver Wafzig, Carola Krüger, Sara García-Gómez, María I. Martínez-Jiménez, Alberto Díaz-Talavera, Daniela Weber, Luis Blanco, Armin Schneider
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/7684ce22eed44d1aa7aa7d8f45a853bc
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