Survival of Enterohemorrhagic Escherichia coli O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on rpoS and Environmental Factors

The consumption of contaminated fresh produce caused outbreaks of enterohemorrhagic (EHEC) Escherichia coli. Agricultural soil might be a reservoir for EHEC strains and represent a contamination source for edible plants. Furthermore, the application of manure as fertilizer is an i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katharina Detert, Herbert Schmidt
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	EHEC/EAEC O104:H4 C227/11Φcu survival agricultural soil <i>rpoS</i> Medicine R
Acceso en línea:	https://doaj.org/article/769857ee10cc4a9895178aea5a25ba61
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:769857ee10cc4a9895178aea5a25ba61
record_format	dspace
spelling	oai:doaj.org-article:769857ee10cc4a9895178aea5a25ba612021-11-25T18:38:17ZSurvival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors10.3390/pathogens101114432076-0817https://doaj.org/article/769857ee10cc4a9895178aea5a25ba612021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1443https://doaj.org/toc/2076-0817The consumption of contaminated fresh produce caused outbreaks of enterohemorrhagic (EHEC) <i>Escherichia coli</i>. Agricultural soil might be a reservoir for EHEC strains and represent a contamination source for edible plants. Furthermore, the application of manure as fertilizer is an important contamination route. Thus, the German fertilizer ordinance prohibits the use of manure 12 weeks before crop harvest to avoid pathogen transmission into the food chain. In this study, the survival of <i>E. coli</i> O104:H4 strain C227/11Φcu in soil microenvironments with either diluvial sand or alluvial loam at two temperatures was investigated for more than 12 weeks. It was analyzed whether the addition of cattle manure extends EHEC survival in these microenvironments. The experiments were additionally performed with isogenic Δ<i>rpoS</i> and Δ<i>fliC</i> deletion mutants of C227/11Φcu. The survival of C227/11Φcu was highest at 4 °C, whereas the soil type had a minor influence. The addition of cattle manure increased the survival at 22 °C. Deletion of <i>rpoS</i> significantly decreased the survival period under all cultivation conditions, whereas <i>fliC</i> deletion did not have any influence. The results of our study demonstrate that EHEC C227/11Φcu is able to survive for more than 12 weeks in soil microenvironments and that RpoS is an important determinant for survival.Katharina DetertHerbert SchmidtMDPI AGarticleEHEC/EAECO104:H4C227/11Φcusurvivalagricultural soil<i>rpoS</i>MedicineRENPathogens, Vol 10, Iss 1443, p 1443 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	EHEC/EAEC O104:H4 C227/11Φcu survival agricultural soil <i>rpoS</i> Medicine R
spellingShingle	EHEC/EAEC O104:H4 C227/11Φcu survival agricultural soil <i>rpoS</i> Medicine R Katharina Detert Herbert Schmidt Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
description	The consumption of contaminated fresh produce caused outbreaks of enterohemorrhagic (EHEC) <i>Escherichia coli</i>. Agricultural soil might be a reservoir for EHEC strains and represent a contamination source for edible plants. Furthermore, the application of manure as fertilizer is an important contamination route. Thus, the German fertilizer ordinance prohibits the use of manure 12 weeks before crop harvest to avoid pathogen transmission into the food chain. In this study, the survival of <i>E. coli</i> O104:H4 strain C227/11Φcu in soil microenvironments with either diluvial sand or alluvial loam at two temperatures was investigated for more than 12 weeks. It was analyzed whether the addition of cattle manure extends EHEC survival in these microenvironments. The experiments were additionally performed with isogenic Δ<i>rpoS</i> and Δ<i>fliC</i> deletion mutants of C227/11Φcu. The survival of C227/11Φcu was highest at 4 °C, whereas the soil type had a minor influence. The addition of cattle manure increased the survival at 22 °C. Deletion of <i>rpoS</i> significantly decreased the survival period under all cultivation conditions, whereas <i>fliC</i> deletion did not have any influence. The results of our study demonstrate that EHEC C227/11Φcu is able to survive for more than 12 weeks in soil microenvironments and that RpoS is an important determinant for survival.
format	article
author	Katharina Detert Herbert Schmidt
author_facet	Katharina Detert Herbert Schmidt
author_sort	Katharina Detert
title	Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
title_short	Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
title_full	Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
title_fullStr	Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
title_full_unstemmed	Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors
title_sort	survival of enterohemorrhagic <i>escherichia coli</i> o104:h4 strain c227/11φcu in agricultural soils depends on <i>rpos</i> and environmental factors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/769857ee10cc4a9895178aea5a25ba61
work_keys_str_mv	AT katharinadetert survivalofenterohemorrhagiciescherichiacoliio104h4strainc22711phcuinagriculturalsoilsdependsonirposiandenvironmentalfactors AT herbertschmidt survivalofenterohemorrhagiciescherichiacoliio104h4strainc22711phcuinagriculturalsoilsdependsonirposiandenvironmentalfactors
_version_	1718410897877106688

Survival of Enterohemorrhagic <i>Escherichia coli</i> O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on <i>rpoS</i> and Environmental Factors

Ejemplares similares