Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile

Huan Yang,1,* Rui Cai,1,* Yangheng Zhang,2,* Yongyan Chen,1 Bing Gu1,3 1Xuzhou Key Laboratory of Laboratory Diagnostics, School of Medical Technology, Xuzhou Medical University, Xuzhou 221004, People’s Republic of China; 2Department of Periodontology, Nanjing Stomatological Hospital of Nan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yang H, Cai R, Zhang Y, Chen Y, Gu B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	clostridium difficile antibacterial effect gold nanoclusters reactive oxygen species Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/76985ebce319487abb86cb7f98fb25f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76985ebce319487abb86cb7f98fb25f2
record_format	dspace
spelling	oai:doaj.org-article:76985ebce319487abb86cb7f98fb25f22021-12-02T11:07:08ZGold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile1178-2013https://doaj.org/article/76985ebce319487abb86cb7f98fb25f22020-08-01T00:00:00Zhttps://www.dovepress.com/gold-nanoclusters-as-an-antibacterial-alternative-against-clostridium--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huan Yang,1,* Rui Cai,1,* Yangheng Zhang,2,* Yongyan Chen,1 Bing Gu1,3 1Xuzhou Key Laboratory of Laboratory Diagnostics, School of Medical Technology, Xuzhou Medical University, Xuzhou 221004, People’s Republic of China; 2Department of Periodontology, Nanjing Stomatological Hospital of Nanjing University School of Medicine, Nanjing 210008, People’s Republic of China; 3Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing GuDepartment of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, People’s Republic of ChinaEmail binggu2015@xzhmu.edu.cnBackground: Clostridium difficile infection (CDI) has become one of the most important factors threatening human health, and about 20– 30% antibiotic-related diarrhea cases and almost all pseudomembranous enteritis cases are related to CDI. The high recurrence of Clostridium difficile (C. difficile) and the emergence of drug resistance make clinical treatment of CDI difficult. Therefore, there is an urgent need to develop a non-antibiotic-alternative therapy against CDI. Gold nanoclusters (AuNCs) can better interact with bacteria due to its ultrasmall size. The aim of the present study was to explore whether AuNCs could be used as an antibacterial agent to kill C. difficile.Methods: AuNCs and C. difficile were co-cultivated in an anaerobic atmosphere to evaluate the bactericidal effect of AuNCs. The bacterial growth rate was estimated by using two concentrations of AuNCs (50 and 100 μM). The damage of AuNCs to C. difficile is detected by SYTOX Green staining methods and SEM image analysis. The mechanism of AuNCs on C. difficile was explored by reactive oxygen species (ROS) detection. The toxic effect of AuNCs on human cells was evaluated by MTT method.Results: AuNCs (100 μM) killed C. difficile drastically. AuNCs increased the release of ROS by about 5 fold and destroyed the membrane integrity of C. difficile cells without causing significant toxic effect on human cells.Conclusion: AuNCs showed great potential as an alternative to traditional antibiotics in killing C. difficile and may prove to be an alternative to treat CDI.Keywords: Clostridium difficile, antibacterial effect, gold nanoclusters, reactive oxygen speciesYang HCai RZhang YChen YGu BDove Medical Pressarticleclostridium difficileantibacterial effectgold nanoclustersreactive oxygen speciesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6401-6408 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	clostridium difficile antibacterial effect gold nanoclusters reactive oxygen species Medicine (General) R5-920
spellingShingle	clostridium difficile antibacterial effect gold nanoclusters reactive oxygen species Medicine (General) R5-920 Yang H Cai R Zhang Y Chen Y Gu B Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
description	Huan Yang,1,* Rui Cai,1,* Yangheng Zhang,2,* Yongyan Chen,1 Bing Gu1,3 1Xuzhou Key Laboratory of Laboratory Diagnostics, School of Medical Technology, Xuzhou Medical University, Xuzhou 221004, People’s Republic of China; 2Department of Periodontology, Nanjing Stomatological Hospital of Nanjing University School of Medicine, Nanjing 210008, People’s Republic of China; 3Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bing GuDepartment of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221006, People’s Republic of ChinaEmail binggu2015@xzhmu.edu.cnBackground: Clostridium difficile infection (CDI) has become one of the most important factors threatening human health, and about 20– 30% antibiotic-related diarrhea cases and almost all pseudomembranous enteritis cases are related to CDI. The high recurrence of Clostridium difficile (C. difficile) and the emergence of drug resistance make clinical treatment of CDI difficult. Therefore, there is an urgent need to develop a non-antibiotic-alternative therapy against CDI. Gold nanoclusters (AuNCs) can better interact with bacteria due to its ultrasmall size. The aim of the present study was to explore whether AuNCs could be used as an antibacterial agent to kill C. difficile.Methods: AuNCs and C. difficile were co-cultivated in an anaerobic atmosphere to evaluate the bactericidal effect of AuNCs. The bacterial growth rate was estimated by using two concentrations of AuNCs (50 and 100 μM). The damage of AuNCs to C. difficile is detected by SYTOX Green staining methods and SEM image analysis. The mechanism of AuNCs on C. difficile was explored by reactive oxygen species (ROS) detection. The toxic effect of AuNCs on human cells was evaluated by MTT method.Results: AuNCs (100 μM) killed C. difficile drastically. AuNCs increased the release of ROS by about 5 fold and destroyed the membrane integrity of C. difficile cells without causing significant toxic effect on human cells.Conclusion: AuNCs showed great potential as an alternative to traditional antibiotics in killing C. difficile and may prove to be an alternative to treat CDI.Keywords: Clostridium difficile, antibacterial effect, gold nanoclusters, reactive oxygen species
format	article
author	Yang H Cai R Zhang Y Chen Y Gu B
author_facet	Yang H Cai R Zhang Y Chen Y Gu B
author_sort	Yang H
title	Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
title_short	Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
title_full	Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
title_fullStr	Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
title_full_unstemmed	Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile
title_sort	gold nanoclusters as an antibacterial alternative against clostridium difficile
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/76985ebce319487abb86cb7f98fb25f2
work_keys_str_mv	AT yangh goldnanoclustersasanantibacterialalternativeagainstclostridiumdifficile AT cair goldnanoclustersasanantibacterialalternativeagainstclostridiumdifficile AT zhangy goldnanoclustersasanantibacterialalternativeagainstclostridiumdifficile AT cheny goldnanoclustersasanantibacterialalternativeagainstclostridiumdifficile AT gub goldnanoclustersasanantibacterialalternativeagainstclostridiumdifficile
_version_	1718396229397774336

Gold Nanoclusters as an Antibacterial Alternative Against Clostridium difficile

Ejemplares similares