2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN

Influenza is a serious public health problem. The ability of the influenza virus to change upon replication is the most serious issue for practical medicine and virology, which can fundamentally alter virus biological properties, such as infectivity and virulence. The high mutational variability of...

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Autores principales:	T. Glebova, N. Klivleyeva, G. Lukmanova, N. Saktaganov, A. Baimukhametova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	influenza virus strain resistance susceptibility sensitivity antiviral drug Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/769e1900a253428091c3e068518267ca
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spelling	oai:doaj.org-article:769e1900a253428091c3e068518267ca2021-11-22T07:09:55Z2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN2220-76192313-739810.15789/2220-7619-ADS-1497https://doaj.org/article/769e1900a253428091c3e068518267ca2019-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1497https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Influenza is a serious public health problem. The ability of the influenza virus to change upon replication is the most serious issue for practical medicine and virology, which can fundamentally alter virus biological properties, such as infectivity and virulence. The high mutational variability of influenza viruses can contribute to rapidly emerging drug resistance. Therefore, the study of antiviral drug sensitivity among influenza viruses is necessary to justify proper drug use for treatment and prevention of influenza infection. The aim of the study was to examine antiviral drug susceptibility of the influenza A/H1N1 and type B virus strains isolated from various regions of Kazakhstan in the years 2018 - 2019. Materials and methods. The susceptibility analysis of 20 strains of influenza A/H1N1 and B viruses was carried out by using chemotherapeutic agents including remantadine, tamiflu, arbidol, and ingavirin. Viruses were cultured in the allantoic cavity of developing 10-day-old chicken embryos for 48 hours at 36° C. The hemagglutinating activity was determined according to the standard method on 96-well plates using 0.75% chicken red blood cell suspension; the infectivity was calculated by the Reed-Muench method. The susceptibility of virus strains to different concentrations of antiviral drugs was evaluated by the level of virus reproductive suppression of 100 lg EID50/0.2 ml in chicken embryos. Statistical analysis was performed using Microsoft Office Excel 2010 software. Results. A study of susceptibility to chemotherapeutic agents demonstrated heterogeneity of the influenza A and B virus population isolated in the 2018–2019 Kazakhstan. The susceptibility to tamiflu was found in all Kazakhstan strains of influenza A/H1N1 virus and three type B strains (inhibitory concentration was 0.44-25.38 μg/mL). The reproduction of most viruses was effectively inhibited by Tamiflu at a concentration of 0.68-3.23 μg/mL. The inhibitory concentration for the three strains of A/H1N1 virus was 7.23-25.38 μg/mL. Remantadine inhibited reproduction of viruses at higher doses (12.60–25.55 μg /mL). All investigated viruses were resistant to arbidol and ingavirin. A single type B influenza virus strain was found to be weakly susceptible to ingavirin. Conclusion. The heterogeneity of the influenza virus population in susceptibility to antiviral drugs suggest a need for constant epidemiological surveillance in order to identify drug-resistant variantsT. GlebovaN. KlivleyevaG. LukmanovaN. SaktaganovA. BaimukhametovaSankt-Peterburg : NIIÈM imeni Pasteraarticleinfluenzavirusstrainresistancesusceptibilitysensitivityantiviral drugInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 0, Iss 0 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	influenza virus strain resistance susceptibility sensitivity antiviral drug Infectious and parasitic diseases RC109-216
spellingShingle	influenza virus strain resistance susceptibility sensitivity antiviral drug Infectious and parasitic diseases RC109-216 T. Glebova N. Klivleyeva G. Lukmanova N. Saktaganov A. Baimukhametova 2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
description	Influenza is a serious public health problem. The ability of the influenza virus to change upon replication is the most serious issue for practical medicine and virology, which can fundamentally alter virus biological properties, such as infectivity and virulence. The high mutational variability of influenza viruses can contribute to rapidly emerging drug resistance. Therefore, the study of antiviral drug sensitivity among influenza viruses is necessary to justify proper drug use for treatment and prevention of influenza infection. The aim of the study was to examine antiviral drug susceptibility of the influenza A/H1N1 and type B virus strains isolated from various regions of Kazakhstan in the years 2018 - 2019. Materials and methods. The susceptibility analysis of 20 strains of influenza A/H1N1 and B viruses was carried out by using chemotherapeutic agents including remantadine, tamiflu, arbidol, and ingavirin. Viruses were cultured in the allantoic cavity of developing 10-day-old chicken embryos for 48 hours at 36° C. The hemagglutinating activity was determined according to the standard method on 96-well plates using 0.75% chicken red blood cell suspension; the infectivity was calculated by the Reed-Muench method. The susceptibility of virus strains to different concentrations of antiviral drugs was evaluated by the level of virus reproductive suppression of 100 lg EID50/0.2 ml in chicken embryos. Statistical analysis was performed using Microsoft Office Excel 2010 software. Results. A study of susceptibility to chemotherapeutic agents demonstrated heterogeneity of the influenza A and B virus population isolated in the 2018–2019 Kazakhstan. The susceptibility to tamiflu was found in all Kazakhstan strains of influenza A/H1N1 virus and three type B strains (inhibitory concentration was 0.44-25.38 μg/mL). The reproduction of most viruses was effectively inhibited by Tamiflu at a concentration of 0.68-3.23 μg/mL. The inhibitory concentration for the three strains of A/H1N1 virus was 7.23-25.38 μg/mL. Remantadine inhibited reproduction of viruses at higher doses (12.60–25.55 μg /mL). All investigated viruses were resistant to arbidol and ingavirin. A single type B influenza virus strain was found to be weakly susceptible to ingavirin. Conclusion. The heterogeneity of the influenza virus population in susceptibility to antiviral drugs suggest a need for constant epidemiological surveillance in order to identify drug-resistant variants
format	article
author	T. Glebova N. Klivleyeva G. Lukmanova N. Saktaganov A. Baimukhametova
author_facet	T. Glebova N. Klivleyeva G. Lukmanova N. Saktaganov A. Baimukhametova
author_sort	T. Glebova
title	2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
title_short	2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
title_full	2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
title_fullStr	2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
title_full_unstemmed	2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN
title_sort	2018 – 2019 antiviral drug sensitivity of the influenza virus strains isolated from various regions of kazakhstan
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/769e1900a253428091c3e068518267ca
work_keys_str_mv	AT tglebova 20182019antiviraldrugsensitivityoftheinfluenzavirusstrainsisolatedfromvariousregionsofkazakhstan AT nklivleyeva 20182019antiviraldrugsensitivityoftheinfluenzavirusstrainsisolatedfromvariousregionsofkazakhstan AT glukmanova 20182019antiviraldrugsensitivityoftheinfluenzavirusstrainsisolatedfromvariousregionsofkazakhstan AT nsaktaganov 20182019antiviraldrugsensitivityoftheinfluenzavirusstrainsisolatedfromvariousregionsofkazakhstan AT abaimukhametova 20182019antiviraldrugsensitivityoftheinfluenzavirusstrainsisolatedfromvariousregionsofkazakhstan
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2018 – 2019 ANTIVIRAL DRUG SENSITIVITY OF THE INFLUENZA VIRUS STRAINS ISOLATED FROM VARIOUS REGIONS OF KAZAKHSTAN

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