Study of camelpox virus pathogenesis in athymic nude mice.

Camelpox virus (CMLV) is the closest known orthopoxvirus genetically related to variola virus. So far, CMLV was restricted to camelids but, recently, three human cases of camelpox have been described in India, highlighting the need to pursue research on its pathogenesis, which has been hampered by t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sophie Duraffour, Patrick Matthys, Joost J van den Oord, Tim De Schutter, Tania Mitera, Robert Snoeck, Graciela Andrei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76a1d5b7a49d4ac5aab40a344604e9ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76a1d5b7a49d4ac5aab40a344604e9ab
record_format	dspace
spelling	oai:doaj.org-article:76a1d5b7a49d4ac5aab40a344604e9ab2021-11-18T06:51:06ZStudy of camelpox virus pathogenesis in athymic nude mice.1932-620310.1371/journal.pone.0021561https://doaj.org/article/76a1d5b7a49d4ac5aab40a344604e9ab2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21738709/?tool=EBIhttps://doaj.org/toc/1932-6203Camelpox virus (CMLV) is the closest known orthopoxvirus genetically related to variola virus. So far, CMLV was restricted to camelids but, recently, three human cases of camelpox have been described in India, highlighting the need to pursue research on its pathogenesis, which has been hampered by the lack of small animal models. Here, we confirm that NMRI immunocompetent mice are resistant to intranasal (i.n.) CMLV infection. However, we demonstrate that CMLV induced a severe disease following i.n. challenge of athymic nude mice, which was accompanied with a failure in gaining weight, leading to euthanasia of the animals. On the other hand, intracutaneous (i.c.) infection resulted in disease development without impacting the body weight evolution. CMLV replication in tissues and body fluids was confirmed in the two models. We further analyzed innate immune and B cell responses induced in the spleen and draining lymph nodes after exposure to CMLV. In both models, strong increases in CD11b(+)F4/80(+) macrophages were seen in the spleen, while neutrophils, NK and B cell responses varied between the routes of infection. In the lymph nodes, the magnitude of CD11c(+)CD8α(+) lymphoid and CD11c(+)CD11b(+) myeloid dendritic cell responses increased in i.n. challenged animals. Analysis of cytokine profiles revealed significant increases of interleukin (IL)-6 and IL-18 in the sera of infected animals, while those of other cytokines were similar to uninfected controls. The efficacy of two antivirals (cidofovir or HPMPC, and its 2, 6-diaminopurine analog) was evaluated in both models. HPMPC was the most effective molecule affording 100% protection from morbidity. It appeared that both treatments did not affect immune cell responses or cytokine expression. In conclusion, we demonstrated that immunodeficient mice are permissive for CMLV propagation. These results provide a basis for studying the pathogenesis of CMLV, as well as for evaluating potential antiviral therapies in an immunodeficiency context.Sophie DuraffourPatrick MatthysJoost J van den OordTim De SchutterTania MiteraRobert SnoeckGraciela AndreiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21561 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophie Duraffour Patrick Matthys Joost J van den Oord Tim De Schutter Tania Mitera Robert Snoeck Graciela Andrei Study of camelpox virus pathogenesis in athymic nude mice.
description	Camelpox virus (CMLV) is the closest known orthopoxvirus genetically related to variola virus. So far, CMLV was restricted to camelids but, recently, three human cases of camelpox have been described in India, highlighting the need to pursue research on its pathogenesis, which has been hampered by the lack of small animal models. Here, we confirm that NMRI immunocompetent mice are resistant to intranasal (i.n.) CMLV infection. However, we demonstrate that CMLV induced a severe disease following i.n. challenge of athymic nude mice, which was accompanied with a failure in gaining weight, leading to euthanasia of the animals. On the other hand, intracutaneous (i.c.) infection resulted in disease development without impacting the body weight evolution. CMLV replication in tissues and body fluids was confirmed in the two models. We further analyzed innate immune and B cell responses induced in the spleen and draining lymph nodes after exposure to CMLV. In both models, strong increases in CD11b(+)F4/80(+) macrophages were seen in the spleen, while neutrophils, NK and B cell responses varied between the routes of infection. In the lymph nodes, the magnitude of CD11c(+)CD8α(+) lymphoid and CD11c(+)CD11b(+) myeloid dendritic cell responses increased in i.n. challenged animals. Analysis of cytokine profiles revealed significant increases of interleukin (IL)-6 and IL-18 in the sera of infected animals, while those of other cytokines were similar to uninfected controls. The efficacy of two antivirals (cidofovir or HPMPC, and its 2, 6-diaminopurine analog) was evaluated in both models. HPMPC was the most effective molecule affording 100% protection from morbidity. It appeared that both treatments did not affect immune cell responses or cytokine expression. In conclusion, we demonstrated that immunodeficient mice are permissive for CMLV propagation. These results provide a basis for studying the pathogenesis of CMLV, as well as for evaluating potential antiviral therapies in an immunodeficiency context.
format	article
author	Sophie Duraffour Patrick Matthys Joost J van den Oord Tim De Schutter Tania Mitera Robert Snoeck Graciela Andrei
author_facet	Sophie Duraffour Patrick Matthys Joost J van den Oord Tim De Schutter Tania Mitera Robert Snoeck Graciela Andrei
author_sort	Sophie Duraffour
title	Study of camelpox virus pathogenesis in athymic nude mice.
title_short	Study of camelpox virus pathogenesis in athymic nude mice.
title_full	Study of camelpox virus pathogenesis in athymic nude mice.
title_fullStr	Study of camelpox virus pathogenesis in athymic nude mice.
title_full_unstemmed	Study of camelpox virus pathogenesis in athymic nude mice.
title_sort	study of camelpox virus pathogenesis in athymic nude mice.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/76a1d5b7a49d4ac5aab40a344604e9ab
work_keys_str_mv	AT sophieduraffour studyofcamelpoxviruspathogenesisinathymicnudemice AT patrickmatthys studyofcamelpoxviruspathogenesisinathymicnudemice AT joostjvandenoord studyofcamelpoxviruspathogenesisinathymicnudemice AT timdeschutter studyofcamelpoxviruspathogenesisinathymicnudemice AT taniamitera studyofcamelpoxviruspathogenesisinathymicnudemice AT robertsnoeck studyofcamelpoxviruspathogenesisinathymicnudemice AT gracielaandrei studyofcamelpoxviruspathogenesisinathymicnudemice
_version_	1718424301662633984

Study of camelpox virus pathogenesis in athymic nude mice.

Ejemplares similares