Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.

Invasion of the trophoblast into the maternal decidua is regulated by both the trophoectoderm and the endometrial stroma, and entails the action of tissue remodeling enzymes. Trophoblast invasion requires the action of metalloproteinases (MMPs) to degrade extracellular matrix (ECM) proteins and in t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carlos Estella, Isabel Herrer, Stuart P Atkinson, Alicia Quiñonero, Sebastián Martínez, Antonio Pellicer, Carlos Simón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76aa47bcd3c54bf8a73d7d42d92c2242
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76aa47bcd3c54bf8a73d7d42d92c2242
record_format	dspace
spelling	oai:doaj.org-article:76aa47bcd3c54bf8a73d7d42d92c22422021-11-18T07:29:16ZInhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.1932-620310.1371/journal.pone.0030508https://doaj.org/article/76aa47bcd3c54bf8a73d7d42d92c22422012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22291969/?tool=EBIhttps://doaj.org/toc/1932-6203Invasion of the trophoblast into the maternal decidua is regulated by both the trophoectoderm and the endometrial stroma, and entails the action of tissue remodeling enzymes. Trophoblast invasion requires the action of metalloproteinases (MMPs) to degrade extracellular matrix (ECM) proteins and in turn, decidual cells express tissue inhibitors of MMPs (TIMPs). The balance between these promoting and restraining factors is a key event for the successful outcome of pregnancy. Gene expression is post-transcriptionally regulated by histone deacetylases (HDACs) that unpacks condensed chromatin activating gene expression. In this study we analyze the effect of histone acetylation on the expression of tissue remodeling enzymes and activity of human endometrial stromal cells (hESCs) related to trophoblast invasion control. Treatment of hESCs with the HDAC inhibitor trichostatin A (TSA) increased the expression of TIMP-1 and TIMP-3 while decreased MMP-2, MMP-9 and uPA and have an inhibitory effect on trophoblast invasion. Moreover, histone acetylation is detected at the promoters of TIMP-1 and TIMP-3 genes in TSA-treated. In addition, in an in vitro decidualized hESCs model, the increase of TIMP-1 and TIMP-3 expression is associated with histone acetylation at the promoters of these genes. Our results demonstrate that histone acetylation disrupt the balance of ECM modulators provoking a restrain of trophoblast invasion. These findings are important as an epigenetic mechanism that can be used to control trophoblast invasion.Carlos EstellaIsabel HerrerStuart P AtkinsonAlicia QuiñoneroSebastián MartínezAntonio PellicerCarlos SimónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30508 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos Estella Isabel Herrer Stuart P Atkinson Alicia Quiñonero Sebastián Martínez Antonio Pellicer Carlos Simón Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
description	Invasion of the trophoblast into the maternal decidua is regulated by both the trophoectoderm and the endometrial stroma, and entails the action of tissue remodeling enzymes. Trophoblast invasion requires the action of metalloproteinases (MMPs) to degrade extracellular matrix (ECM) proteins and in turn, decidual cells express tissue inhibitors of MMPs (TIMPs). The balance between these promoting and restraining factors is a key event for the successful outcome of pregnancy. Gene expression is post-transcriptionally regulated by histone deacetylases (HDACs) that unpacks condensed chromatin activating gene expression. In this study we analyze the effect of histone acetylation on the expression of tissue remodeling enzymes and activity of human endometrial stromal cells (hESCs) related to trophoblast invasion control. Treatment of hESCs with the HDAC inhibitor trichostatin A (TSA) increased the expression of TIMP-1 and TIMP-3 while decreased MMP-2, MMP-9 and uPA and have an inhibitory effect on trophoblast invasion. Moreover, histone acetylation is detected at the promoters of TIMP-1 and TIMP-3 genes in TSA-treated. In addition, in an in vitro decidualized hESCs model, the increase of TIMP-1 and TIMP-3 expression is associated with histone acetylation at the promoters of these genes. Our results demonstrate that histone acetylation disrupt the balance of ECM modulators provoking a restrain of trophoblast invasion. These findings are important as an epigenetic mechanism that can be used to control trophoblast invasion.
format	article
author	Carlos Estella Isabel Herrer Stuart P Atkinson Alicia Quiñonero Sebastián Martínez Antonio Pellicer Carlos Simón
author_facet	Carlos Estella Isabel Herrer Stuart P Atkinson Alicia Quiñonero Sebastián Martínez Antonio Pellicer Carlos Simón
author_sort	Carlos Estella
title	Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
title_short	Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
title_full	Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
title_fullStr	Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
title_full_unstemmed	Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
title_sort	inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/76aa47bcd3c54bf8a73d7d42d92c2242
work_keys_str_mv	AT carlosestella inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT isabelherrer inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT stuartpatkinson inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT aliciaquinonero inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT sebastianmartinez inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT antoniopellicer inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion AT carlossimon inhibitionofhistonedeacetylaseactivityinhumanendometrialstromalcellspromotesextracellularmatrixremodellingandlimitsembryoinvasion
_version_	1718423416172707840

Inhibition of histone deacetylase activity in human endometrial stromal cells promotes extracellular matrix remodelling and limits embryo invasion.

Ejemplares similares