Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays

Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays

Nowadays, many commercial kits allowing the detection of digestive parasites by DNA amplification methods have been developed, including simplex PCR assays (SimpPCRa) allowing the identification of a single parasite, and multiplex PCR assays (MultPCRa) allowing the identification of several parasite...

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Autores principales: Louise Basmaciyan, Alexandre François, Anne Vincent, Stéphane Valot, Alain Bonnin, Damien Costa, Romy Razakandrainibe, Florent Morio, Loic Favennec, Frédéric Dalle
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
intestinal parasitic diseases
<i>Entamoeba</i> spp.
<i>Giardia intestinalis</i>
<i>Cryptosporidium</i> spp.
diagnosis
DNA amplification
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/76b1216619f041b78f0350279372c902
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spelling oai:doaj.org-article:76b1216619f041b78f0350279372c9022021-11-25T18:25:09ZCommercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays10.3390/microorganisms91123252076-2607https://doaj.org/article/76b1216619f041b78f0350279372c9022021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2325https://doaj.org/toc/2076-2607Nowadays, many commercial kits allowing the detection of digestive parasites by DNA amplification methods have been developed, including simplex PCR assays (SimpPCRa) allowing the identification of a single parasite, and multiplex PCR assays (MultPCRa) allowing the identification of several parasites at once. Thus, aimed at improving the diagnosis of intestinal protozoal infections, it is essential to evaluate the performances of these new tools. A total of 174 DNA samples collected between 2007 and 2017 were retrospectively included in this study. Performances of four commercial SimpPCRa (i.e., CerTest-VIASURE<sup>TM</sup>) and three MultPCRa (i.e., CerTest-VIASURE<sup>TM</sup>, FAST-TRACK-Diagnostics-FTD-Stool-Parasite<sup>TM</sup> and DIAGENODE-Gastroenteritis/Parasite-panel-I<sup>TM</sup>) were evaluated for the detection of <i>Cryptosporidium</i> spp., <i>Entamoeba</i> spp., and <i>Giardia intestinalis</i> in stool samples compared to our routinely used in-house SimpPCRa. Globally, the SimpPCRa showed better sensitivity/specificity for the detection of <i>G. intestinalis</i>, <i>E. histolytica</i>, <i>E. dispar,</i> and <i>Cryptosporidium</i> spp. (i.e., 96.9/93.6%; 100/100%; 95.5/100%; and 100/99.3%, respectively), compared to the three commercial MultPCRa tested. All in all, we showed that MultPCRa offer an interesting alternative for the detection of protozoans in stool samples depending on the clinical context.Louise BasmaciyanAlexandre FrançoisAnne VincentStéphane ValotAlain BonninDamien CostaRomy RazakandrainibeFlorent MorioLoic FavennecFrédéric DalleMDPI AGarticleintestinal parasitic diseases<i>Entamoeba</i> spp.<i>Giardia intestinalis</i><i>Cryptosporidium</i> spp.diagnosisDNA amplificationBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2325, p 2325 (2021)
institution DOAJ
collection DOAJ
language EN
topic intestinal parasitic diseases
<i>Entamoeba</i> spp.
<i>Giardia intestinalis</i>
<i>Cryptosporidium</i> spp.
diagnosis
DNA amplification
Biology (General)
QH301-705.5
spellingShingle intestinal parasitic diseases
<i>Entamoeba</i> spp.
<i>Giardia intestinalis</i>
<i>Cryptosporidium</i> spp.
diagnosis
DNA amplification
Biology (General)
QH301-705.5
Louise Basmaciyan
Alexandre François
Anne Vincent
Stéphane Valot
Alain Bonnin
Damien Costa
Romy Razakandrainibe
Florent Morio
Loic Favennec
Frédéric Dalle
Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
description Nowadays, many commercial kits allowing the detection of digestive parasites by DNA amplification methods have been developed, including simplex PCR assays (SimpPCRa) allowing the identification of a single parasite, and multiplex PCR assays (MultPCRa) allowing the identification of several parasites at once. Thus, aimed at improving the diagnosis of intestinal protozoal infections, it is essential to evaluate the performances of these new tools. A total of 174 DNA samples collected between 2007 and 2017 were retrospectively included in this study. Performances of four commercial SimpPCRa (i.e., CerTest-VIASURE<sup>TM</sup>) and three MultPCRa (i.e., CerTest-VIASURE<sup>TM</sup>, FAST-TRACK-Diagnostics-FTD-Stool-Parasite<sup>TM</sup> and DIAGENODE-Gastroenteritis/Parasite-panel-I<sup>TM</sup>) were evaluated for the detection of <i>Cryptosporidium</i> spp., <i>Entamoeba</i> spp., and <i>Giardia intestinalis</i> in stool samples compared to our routinely used in-house SimpPCRa. Globally, the SimpPCRa showed better sensitivity/specificity for the detection of <i>G. intestinalis</i>, <i>E. histolytica</i>, <i>E. dispar,</i> and <i>Cryptosporidium</i> spp. (i.e., 96.9/93.6%; 100/100%; 95.5/100%; and 100/99.3%, respectively), compared to the three commercial MultPCRa tested. All in all, we showed that MultPCRa offer an interesting alternative for the detection of protozoans in stool samples depending on the clinical context.
format article
author Louise Basmaciyan
Alexandre François
Anne Vincent
Stéphane Valot
Alain Bonnin
Damien Costa
Romy Razakandrainibe
Florent Morio
Loic Favennec
Frédéric Dalle
author_facet Louise Basmaciyan
Alexandre François
Anne Vincent
Stéphane Valot
Alain Bonnin
Damien Costa
Romy Razakandrainibe
Florent Morio
Loic Favennec
Frédéric Dalle
author_sort Louise Basmaciyan
title Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
title_short Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
title_full Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
title_fullStr Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
title_full_unstemmed Commercial Simplex and Multiplex PCR Assays for the Detection of Intestinal Parasites <i>Giardia intestinalis</i>, <i>Entamoeba</i> spp., and <i>Cryptosporidium</i> spp.: Comparative Evaluation of Seven Commercial PCR Kits with Routine In-House Simplex PCR Assays
title_sort commercial simplex and multiplex pcr assays for the detection of intestinal parasites <i>giardia intestinalis</i>, <i>entamoeba</i> spp., and <i>cryptosporidium</i> spp.: comparative evaluation of seven commercial pcr kits with routine in-house simplex pcr assays
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/76b1216619f041b78f0350279372c902
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