Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.

Self-compatibility has become the primary objective of most prune (Prunus domestica) breeding programs in order to avoid the problems related to the gametophytic self-incompatibility (GSI) system present in this crop. GSI is typically under the control of a specific locus., known as the S-locus., wh...

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Autores principales:	Angel Fernandez I Marti, Sarah Castro, Theodore M DeJong, Richard S Dodd
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76b66b9ec6dd44bd93adc349df583f73
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spelling	oai:doaj.org-article:76b66b9ec6dd44bd93adc349df583f732021-12-02T20:05:36ZEvaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.1932-620310.1371/journal.pone.0251305https://doaj.org/article/76b66b9ec6dd44bd93adc349df583f732021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251305https://doaj.org/toc/1932-6203Self-compatibility has become the primary objective of most prune (Prunus domestica) breeding programs in order to avoid the problems related to the gametophytic self-incompatibility (GSI) system present in this crop. GSI is typically under the control of a specific locus., known as the S-locus., which contains at least two genes. The first gene encodes glycoproteins with RNase activity in the pistils., and the second is an SFB gene expressed in the pollen. There is limited information on genetics of SI/SC in prune and in comparison., with other Prunus species, cloning., sequencing and discovery of different S-alleles is very scarce. Clear information about S-alleles can be used for molecular identification and characterization of the S-haplotypes. We determined the S-alleles of 36 cultivars and selections using primers that revealed 17 new alleles. In addition, our study describes for the first time the association and design of a molecular marker for self-compatibility in P. domestica. Our phylogenetic tree showed that the S-alleles are spread across the phylogeny, suggesting that like previous alleles detected in the Rosaceae., they were of trans-specific origin. We provide for the first time 3D models for the P. domestica SI RNase alleles as well as in other Prunus species, including P. salicina (Japanese plum), P. avium (cherry), P. armeniaca (apricot), P. cerasifera and P. spinosa.Angel Fernandez I MartiSarah CastroTheodore M DeJongRichard S DoddPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251305 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angel Fernandez I Marti Sarah Castro Theodore M DeJong Richard S Dodd Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
description	Self-compatibility has become the primary objective of most prune (Prunus domestica) breeding programs in order to avoid the problems related to the gametophytic self-incompatibility (GSI) system present in this crop. GSI is typically under the control of a specific locus., known as the S-locus., which contains at least two genes. The first gene encodes glycoproteins with RNase activity in the pistils., and the second is an SFB gene expressed in the pollen. There is limited information on genetics of SI/SC in prune and in comparison., with other Prunus species, cloning., sequencing and discovery of different S-alleles is very scarce. Clear information about S-alleles can be used for molecular identification and characterization of the S-haplotypes. We determined the S-alleles of 36 cultivars and selections using primers that revealed 17 new alleles. In addition, our study describes for the first time the association and design of a molecular marker for self-compatibility in P. domestica. Our phylogenetic tree showed that the S-alleles are spread across the phylogeny, suggesting that like previous alleles detected in the Rosaceae., they were of trans-specific origin. We provide for the first time 3D models for the P. domestica SI RNase alleles as well as in other Prunus species, including P. salicina (Japanese plum), P. avium (cherry), P. armeniaca (apricot), P. cerasifera and P. spinosa.
format	article
author	Angel Fernandez I Marti Sarah Castro Theodore M DeJong Richard S Dodd
author_facet	Angel Fernandez I Marti Sarah Castro Theodore M DeJong Richard S Dodd
author_sort	Angel Fernandez I Marti
title	Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
title_short	Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
title_full	Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
title_fullStr	Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
title_full_unstemmed	Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.
title_sort	evaluation of the s-locus in prunus domestica, characterization, phylogeny and 3d modelling.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/76b66b9ec6dd44bd93adc349df583f73
work_keys_str_mv	AT angelfernandezimarti evaluationoftheslocusinprunusdomesticacharacterizationphylogenyand3dmodelling AT sarahcastro evaluationoftheslocusinprunusdomesticacharacterizationphylogenyand3dmodelling AT theodoremdejong evaluationoftheslocusinprunusdomesticacharacterizationphylogenyand3dmodelling AT richardsdodd evaluationoftheslocusinprunusdomesticacharacterizationphylogenyand3dmodelling
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Evaluation of the S-locus in Prunus domestica, characterization, phylogeny and 3D modelling.

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