Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside and reveal mechanistic features of Na+-aspartate s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gianluca Trinco, Valentina Arkhipova, Alisa A. Garaeva, Cedric A. J. Hutter, Markus A. Seeger, Albert Guskov, Dirk J. Slotboom
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/76bc5140efca4c9482b7af7a5ab080f1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76bc5140efca4c9482b7af7a5ab080f1
record_format	dspace
spelling	oai:doaj.org-article:76bc5140efca4c9482b7af7a5ab080f12021-12-02T16:04:30ZKinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk10.1038/s42003-021-02267-y2399-3642https://doaj.org/article/76bc5140efca4c9482b7af7a5ab080f12021-06-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02267-yhttps://doaj.org/toc/2399-3642Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside and reveal mechanistic features of Na+-aspartate symport that cannot be observed in detergent solution.Gianluca TrincoValentina ArkhipovaAlisa A. GaraevaCedric A. J. HutterMarkus A. SeegerAlbert GuskovDirk J. SlotboomNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Gianluca Trinco Valentina Arkhipova Alisa A. Garaeva Cedric A. J. Hutter Markus A. Seeger Albert Guskov Dirk J. Slotboom Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
description	Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside and reveal mechanistic features of Na+-aspartate symport that cannot be observed in detergent solution.
format	article
author	Gianluca Trinco Valentina Arkhipova Alisa A. Garaeva Cedric A. J. Hutter Markus A. Seeger Albert Guskov Dirk J. Slotboom
author_facet	Gianluca Trinco Valentina Arkhipova Alisa A. Garaeva Cedric A. J. Hutter Markus A. Seeger Albert Guskov Dirk J. Slotboom
author_sort	Gianluca Trinco
title	Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
title_short	Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
title_full	Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
title_fullStr	Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
title_full_unstemmed	Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk
title_sort	kinetic mechanism of na+-coupled aspartate transport catalyzed by glttk
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/76bc5140efca4c9482b7af7a5ab080f1
work_keys_str_mv	AT gianlucatrinco kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT valentinaarkhipova kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT alisaagaraeva kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT cedricajhutter kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT markusaseeger kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT albertguskov kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk AT dirkjslotboom kineticmechanismofnacoupledaspartatetransportcatalyzedbyglttk
_version_	1718385214716116992

Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

Ejemplares similares