PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types

Abstract To screen the complex central nervous system (CNS) injury responses, we created a quadruple-labelled ‘PrismPlus’ mouse line with a genetically encoded distinct fluorescent tag in oligodendrocytes, microglia, neurons, and astrocytes. Cx3cr1-gfp and Prism mice originally developed by Jung et...

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Autores principales:	Janak Gaire, Heui Chang Lee, Ray Ward, Seth Currlin, Andrew J. Woolley, Jason E. Coleman, Justin C. Williams, Kevin J. Otto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76bd553fab6f4f92a3468df02e91eaa0
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spelling	oai:doaj.org-article:76bd553fab6f4f92a3468df02e91eaa02021-12-02T12:32:59ZPrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types10.1038/s41598-018-25208-y2045-2322https://doaj.org/article/76bd553fab6f4f92a3468df02e91eaa02018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25208-yhttps://doaj.org/toc/2045-2322Abstract To screen the complex central nervous system (CNS) injury responses, we created a quadruple-labelled ‘PrismPlus’ mouse line with a genetically encoded distinct fluorescent tag in oligodendrocytes, microglia, neurons, and astrocytes. Cx3cr1-gfp and Prism mice originally developed by Jung et al., 2000 and Dougherty et al., 2012, respectively, were cross-bred. First, we confirmed the presence of fluorophores in appropriate cell types in PrismPlus mice. PrismPlus mice were then used to examine the cellular responses to brain implanted micro-devices. We observed an increase in microglial response at earlier time points as compared to 4 weeks, a progressive astrocytic response, and fewer neurons at the vicinity of an implanted device. These results are similar to what has been described in literature using other rodent strains, previously attainable only through time-consuming and variable immunohistochemistry methods. Finally, we demonstrate the compatibility of PrismPlus brain tissue with CLARITY, an advanced tissue clearing technique, opening the door to future thick tissue imaging studies. This report confirms PrismPlus transgenic fluorescence and highlights the utility of these mice to study CNS injuries. The work herein seeks to establish a novel transgenic mouse line to improve experimental scope, consistency, and efficiency for CNS researchers.Janak GaireHeui Chang LeeRay WardSeth CurrlinAndrew J. WoolleyJason E. ColemanJustin C. WilliamsKevin J. OttoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janak Gaire Heui Chang Lee Ray Ward Seth Currlin Andrew J. Woolley Jason E. Coleman Justin C. Williams Kevin J. Otto PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
description	Abstract To screen the complex central nervous system (CNS) injury responses, we created a quadruple-labelled ‘PrismPlus’ mouse line with a genetically encoded distinct fluorescent tag in oligodendrocytes, microglia, neurons, and astrocytes. Cx3cr1-gfp and Prism mice originally developed by Jung et al., 2000 and Dougherty et al., 2012, respectively, were cross-bred. First, we confirmed the presence of fluorophores in appropriate cell types in PrismPlus mice. PrismPlus mice were then used to examine the cellular responses to brain implanted micro-devices. We observed an increase in microglial response at earlier time points as compared to 4 weeks, a progressive astrocytic response, and fewer neurons at the vicinity of an implanted device. These results are similar to what has been described in literature using other rodent strains, previously attainable only through time-consuming and variable immunohistochemistry methods. Finally, we demonstrate the compatibility of PrismPlus brain tissue with CLARITY, an advanced tissue clearing technique, opening the door to future thick tissue imaging studies. This report confirms PrismPlus transgenic fluorescence and highlights the utility of these mice to study CNS injuries. The work herein seeks to establish a novel transgenic mouse line to improve experimental scope, consistency, and efficiency for CNS researchers.
format	article
author	Janak Gaire Heui Chang Lee Ray Ward Seth Currlin Andrew J. Woolley Jason E. Coleman Justin C. Williams Kevin J. Otto
author_facet	Janak Gaire Heui Chang Lee Ray Ward Seth Currlin Andrew J. Woolley Jason E. Coleman Justin C. Williams Kevin J. Otto
author_sort	Janak Gaire
title	PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
title_short	PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
title_full	PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
title_fullStr	PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
title_full_unstemmed	PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
title_sort	prismplus: a mouse line expressing distinct fluorophores in four different brain cell types
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/76bd553fab6f4f92a3468df02e91eaa0
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PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types

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