Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS

Yeast vacuolar membrane fusion has been reconstituted with R, Qa, Qb, and Qc-family SNAREs, Sec17/αSNAP, Sec18/NSF, and the hexameric HOPS complex. HOPS tethers membranes and catalyzes SNARE assembly into RQaQbQc trans-complexes which zipper through their SNARE domains to promote fusion. Previously,...

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Autores principales:	Hongki Song, William T Wickner
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	membrane fusion yeast vacuole SNAREs HOPS Sec17 Sec18 Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/76c2c7b3397643d08d5dc1cdb337dee3
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spelling	oai:doaj.org-article:76c2c7b3397643d08d5dc1cdb337dee32021-11-11T15:37:41ZFusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS10.7554/eLife.732402050-084Xe73240https://doaj.org/article/76c2c7b3397643d08d5dc1cdb337dee32021-10-01T00:00:00Zhttps://elifesciences.org/articles/73240https://doaj.org/toc/2050-084XYeast vacuolar membrane fusion has been reconstituted with R, Qa, Qb, and Qc-family SNAREs, Sec17/αSNAP, Sec18/NSF, and the hexameric HOPS complex. HOPS tethers membranes and catalyzes SNARE assembly into RQaQbQc trans-complexes which zipper through their SNARE domains to promote fusion. Previously, we demonstrated that Sec17 and Sec18 can bypass the requirement of complete zippering for fusion (Song et al., 2021), but it has been unclear whether this activity of Sec17 and Sec18 is directly coupled to HOPS. HOPS can be replaced for fusion by a synthetic tether when the three Q-SNAREs are pre-assembled. We now report that fusion intermediates with arrested SNARE zippering, formed with a synthetic tether but without HOPS, support Sec17/Sec18-triggered fusion. This zippering-bypass fusion is thus a direct result of Sec17 and Sec18 interactions: with each other, with the platform of partially zippered SNAREs, and with the apposed tethered membranes. As these fusion elements are shared among all exocytic and endocytic traffic, Sec17 and Sec18 may have a general role in directly promoting fusion.Hongki SongWilliam T WicknereLife Sciences Publications Ltdarticlemembrane fusionyeast vacuoleSNAREsHOPSSec17Sec18MedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	membrane fusion yeast vacuole SNAREs HOPS Sec17 Sec18 Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	membrane fusion yeast vacuole SNAREs HOPS Sec17 Sec18 Medicine R Science Q Biology (General) QH301-705.5 Hongki Song William T Wickner Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
description	Yeast vacuolar membrane fusion has been reconstituted with R, Qa, Qb, and Qc-family SNAREs, Sec17/αSNAP, Sec18/NSF, and the hexameric HOPS complex. HOPS tethers membranes and catalyzes SNARE assembly into RQaQbQc trans-complexes which zipper through their SNARE domains to promote fusion. Previously, we demonstrated that Sec17 and Sec18 can bypass the requirement of complete zippering for fusion (Song et al., 2021), but it has been unclear whether this activity of Sec17 and Sec18 is directly coupled to HOPS. HOPS can be replaced for fusion by a synthetic tether when the three Q-SNAREs are pre-assembled. We now report that fusion intermediates with arrested SNARE zippering, formed with a synthetic tether but without HOPS, support Sec17/Sec18-triggered fusion. This zippering-bypass fusion is thus a direct result of Sec17 and Sec18 interactions: with each other, with the platform of partially zippered SNAREs, and with the apposed tethered membranes. As these fusion elements are shared among all exocytic and endocytic traffic, Sec17 and Sec18 may have a general role in directly promoting fusion.
format	article
author	Hongki Song William T Wickner
author_facet	Hongki Song William T Wickner
author_sort	Hongki Song
title	Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
title_short	Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
title_full	Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
title_fullStr	Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
title_full_unstemmed	Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS
title_sort	fusion of tethered membranes can be driven by sec18/nsf and sec17/αsnap without hops
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/76c2c7b3397643d08d5dc1cdb337dee3
work_keys_str_mv	AT hongkisong fusionoftetheredmembranescanbedrivenbysec18nsfandsec17asnapwithouthops AT williamtwickner fusionoftetheredmembranescanbedrivenbysec18nsfandsec17asnapwithouthops
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Fusion of tethered membranes can be driven by Sec18/NSF and Sec17/αSNAP without HOPS

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