Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway
Abundant evidence proves the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) in the treatment of diabetes mellitus. However, the problems have not been solved that viability of ADMSCs were inconsistent and the cells quickly undergo senescence after in vitro cell culture. In add...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:76c891b169944520b9e708460a3f76f22021-12-03T09:11:42ZMelatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway2296-634X10.3389/fcell.2021.722365https://doaj.org/article/76c891b169944520b9e708460a3f76f22021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.722365/fullhttps://doaj.org/toc/2296-634XAbundant evidence proves the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) in the treatment of diabetes mellitus. However, the problems have not been solved that viability of ADMSCs were inconsistent and the cells quickly undergo senescence after in vitro cell culture. In addition, the therapeutic effect of ADMSCs is still not satisfactory. In this study, melatonin (MLT) was added to canine ADMSC culture medium, and the treated cells were used to treat type 2 diabetes mellitus (T2DM). Our research reveals that adding MLT to ADMSC culture medium can promote the viability of ADMSCs. This effect depends on the binding of MLT and MLT receptors, which activates the transforming growth factor β (TGF-β) pathway and then changes the cell cycle of ADMSCs and improves the viability of ADMSCs. Since ADMSCs were found to be used to treat T2DM by anti-inflammatory and anti-endoplasmic reticulum (ER) stress capabilities, our data demonstrate that MLT augment several effects of ADMSCs in remission hyperglycemia, insulin resistance, and liver glycogen metabolism in T2DM patients. This suggest that ADMSCs and MLT-ADMSCs is safe and vabulable for pet clinic.Balun LiXuedi ChengAili AierkenJiaxin DuJiaxin DuWenlai HeMengfei ZhangNing TanZheng KouSha PengWenwen JiaHaiyang TangHaiyang TangJinlian HuaFrontiers Media S.A.articlemelatoninadipose-derived mesenchymal stem cellstype 2 diabetes mellitusTGF-βinflammationcanineBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021) |
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EN |
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melatonin adipose-derived mesenchymal stem cells type 2 diabetes mellitus TGF-β inflammation canine Biology (General) QH301-705.5 |
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melatonin adipose-derived mesenchymal stem cells type 2 diabetes mellitus TGF-β inflammation canine Biology (General) QH301-705.5 Balun Li Xuedi Cheng Aili Aierken Jiaxin Du Jiaxin Du Wenlai He Mengfei Zhang Ning Tan Zheng Kou Sha Peng Wenwen Jia Haiyang Tang Haiyang Tang Jinlian Hua Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| description |
Abundant evidence proves the therapeutic effect of adipose-derived mesenchymal stem cells (ADMSCs) in the treatment of diabetes mellitus. However, the problems have not been solved that viability of ADMSCs were inconsistent and the cells quickly undergo senescence after in vitro cell culture. In addition, the therapeutic effect of ADMSCs is still not satisfactory. In this study, melatonin (MLT) was added to canine ADMSC culture medium, and the treated cells were used to treat type 2 diabetes mellitus (T2DM). Our research reveals that adding MLT to ADMSC culture medium can promote the viability of ADMSCs. This effect depends on the binding of MLT and MLT receptors, which activates the transforming growth factor β (TGF-β) pathway and then changes the cell cycle of ADMSCs and improves the viability of ADMSCs. Since ADMSCs were found to be used to treat T2DM by anti-inflammatory and anti-endoplasmic reticulum (ER) stress capabilities, our data demonstrate that MLT augment several effects of ADMSCs in remission hyperglycemia, insulin resistance, and liver glycogen metabolism in T2DM patients. This suggest that ADMSCs and MLT-ADMSCs is safe and vabulable for pet clinic. |
| format |
article |
| author |
Balun Li Xuedi Cheng Aili Aierken Jiaxin Du Jiaxin Du Wenlai He Mengfei Zhang Ning Tan Zheng Kou Sha Peng Wenwen Jia Haiyang Tang Haiyang Tang Jinlian Hua |
| author_facet |
Balun Li Xuedi Cheng Aili Aierken Jiaxin Du Jiaxin Du Wenlai He Mengfei Zhang Ning Tan Zheng Kou Sha Peng Wenwen Jia Haiyang Tang Haiyang Tang Jinlian Hua |
| author_sort |
Balun Li |
| title |
Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| title_short |
Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| title_full |
Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| title_fullStr |
Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| title_full_unstemmed |
Melatonin Promotes the Therapeutic Effect of Mesenchymal Stem Cells on Type 2 Diabetes Mellitus by Regulating TGF-β Pathway |
| title_sort |
melatonin promotes the therapeutic effect of mesenchymal stem cells on type 2 diabetes mellitus by regulating tgf-β pathway |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/76c891b169944520b9e708460a3f76f2 |
| work_keys_str_mv |
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