Investigation of cellular responses upon interaction with silver nanoparticles

Ramesh Subbiah,1,2 Seong Beom Jeon,3,4 Kwideok Park,1,2 Sang Jung Ahn,4,5 Kyusik Yun3 1Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejon, 3Department of Bionanotechnology, Gachon Unive...

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Autores principales:	Subbiah R, Jeon SB, Park K, Ahn SJ, Yun KS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/76ce0571c33c4be2baffabeba589e629
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spelling	oai:doaj.org-article:76ce0571c33c4be2baffabeba589e6292021-12-02T01:07:46ZInvestigation of cellular responses upon interaction with silver nanoparticles1178-2013https://doaj.org/article/76ce0571c33c4be2baffabeba589e6292015-08-01T00:00:00Zhttp://www.dovepress.com/investigation-of-cellular-responses-upon-interaction-with-silver-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ramesh Subbiah,1,2 Seong Beom Jeon,3,4 Kwideok Park,1,2 Sang Jung Ahn,4,5 Kyusik Yun3 1Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejon, 3Department of Bionanotechnology, Gachon University, Gyeonggi-do, 4Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, 5Major of Nano Science, Korea University of Science and Technology, Daejeon, Republic of Korea Abstract: In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from ≤80% at a high AgNPs dose (40 µg/mL) to >95% at a low dose (10 µg/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P<0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics-based biomarker for the evaluation of NP-dependent cytotoxicity and cytopathology. Keywords: AFM, roughness, nanoindentation, biomarker, cytotoxicity, biomechanicsSubbiah RJeon SBPark KAhn SJYun KSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 191-201 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Subbiah R Jeon SB Park K Ahn SJ Yun KS Investigation of cellular responses upon interaction with silver nanoparticles
description	Ramesh Subbiah,1,2 Seong Beom Jeon,3,4 Kwideok Park,1,2 Sang Jung Ahn,4,5 Kyusik Yun3 1Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 2Department of Biomedical Engineering, Korea University of Science and Technology, Daejon, 3Department of Bionanotechnology, Gachon University, Gyeonggi-do, 4Centre for Advanced Instrumentation, Korea Research Institute of Standard and Science, 5Major of Nano Science, Korea University of Science and Technology, Daejeon, Republic of Korea Abstract: In order for nanoparticles (NPs) to be applied in the biomedical field, a thorough investigation of their interactions with biological systems is required. Although this is a growing area of research, there is a paucity of comprehensive data in cell-based studies. To address this, we analyzed the physicomechanical responses of human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3), and human bone marrow stromal cells (HS-5), following their interaction with silver nanoparticles (AgNPs). When compared with kanamycin, AgNPs exhibited moderate antibacterial activity. Cell viability ranged from ≤80% at a high AgNPs dose (40 µg/mL) to >95% at a low dose (10 µg/mL). We also used atomic force microscopy-coupled force spectroscopy to evaluate the biophysical and biomechanical properties of cells. This revealed that AgNPs treatment increased the surface roughness (P<0.001) and stiffness (P<0.001) of cells. Certain cellular changes are likely due to interaction of the AgNPs with the cell surface. The degree to which cellular morphology was altered directly proportional to the level of AgNP-induced cytotoxicity. Together, these data suggest that atomic force microscopy can be used as a potential tool to develop a biomechanics-based biomarker for the evaluation of NP-dependent cytotoxicity and cytopathology. Keywords: AFM, roughness, nanoindentation, biomarker, cytotoxicity, biomechanics
format	article
author	Subbiah R Jeon SB Park K Ahn SJ Yun KS
author_facet	Subbiah R Jeon SB Park K Ahn SJ Yun KS
author_sort	Subbiah R
title	Investigation of cellular responses upon interaction with silver nanoparticles
title_short	Investigation of cellular responses upon interaction with silver nanoparticles
title_full	Investigation of cellular responses upon interaction with silver nanoparticles
title_fullStr	Investigation of cellular responses upon interaction with silver nanoparticles
title_full_unstemmed	Investigation of cellular responses upon interaction with silver nanoparticles
title_sort	investigation of cellular responses upon interaction with silver nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/76ce0571c33c4be2baffabeba589e629
work_keys_str_mv	AT subbiahr investigationofcellularresponsesuponinteractionwithsilvernanoparticles AT jeonsb investigationofcellularresponsesuponinteractionwithsilvernanoparticles AT parkk investigationofcellularresponsesuponinteractionwithsilvernanoparticles AT ahnsj investigationofcellularresponsesuponinteractionwithsilvernanoparticles AT yunks investigationofcellularresponsesuponinteractionwithsilvernanoparticles
_version_	1718403265016627200

Investigation of cellular responses upon interaction with silver nanoparticles

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