Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology,...

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Autores principales:	Carlos Alberto Oliveira Biagi, Sarah Santiloni Cury, Cleidson Pádua Alves, Nabil Rabhi, Wilson Araujo Silva, Stephen R. Farmer, Robson Francisco Carvalho, Miguel Luiz Batista
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mature adipocyte plasticity adipocyte subpopulations cellular compartment prediction transcriptional factors thermogenic treatment Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/76db3ac020a8415991ac6da302fcb0de
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spelling	oai:doaj.org-article:76db3ac020a8415991ac6da302fcb0de2021-11-25T17:11:13ZMultidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response10.3390/cells101130732073-4409https://doaj.org/article/76db3ac020a8415991ac6da302fcb0de2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3073https://doaj.org/toc/2073-4409Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.Carlos Alberto Oliveira BiagiSarah Santiloni CuryCleidson Pádua AlvesNabil RabhiWilson Araujo SilvaStephen R. FarmerRobson Francisco CarvalhoMiguel Luiz BatistaMDPI AGarticlemature adipocyte plasticityadipocyte subpopulationscellular compartment predictiontranscriptional factorsthermogenic treatmentBiology (General)QH301-705.5ENCells, Vol 10, Iss 3073, p 3073 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mature adipocyte plasticity adipocyte subpopulations cellular compartment prediction transcriptional factors thermogenic treatment Biology (General) QH301-705.5
spellingShingle	mature adipocyte plasticity adipocyte subpopulations cellular compartment prediction transcriptional factors thermogenic treatment Biology (General) QH301-705.5 Carlos Alberto Oliveira Biagi Sarah Santiloni Cury Cleidson Pádua Alves Nabil Rabhi Wilson Araujo Silva Stephen R. Farmer Robson Francisco Carvalho Miguel Luiz Batista Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
description	Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.
format	article
author	Carlos Alberto Oliveira Biagi Sarah Santiloni Cury Cleidson Pádua Alves Nabil Rabhi Wilson Araujo Silva Stephen R. Farmer Robson Francisco Carvalho Miguel Luiz Batista
author_facet	Carlos Alberto Oliveira Biagi Sarah Santiloni Cury Cleidson Pádua Alves Nabil Rabhi Wilson Araujo Silva Stephen R. Farmer Robson Francisco Carvalho Miguel Luiz Batista
author_sort	Carlos Alberto Oliveira Biagi
title	Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
title_short	Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
title_full	Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
title_fullStr	Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
title_full_unstemmed	Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response
title_sort	multidimensional single-nuclei rna-seq reconstruction of adipose tissue reveals adipocyte plasticity underlying thermogenic response
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/76db3ac020a8415991ac6da302fcb0de
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Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

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