Detection of pro angiogenic and inflammatory biomarkers in patients with CKD

Abstract Cardiovascular disease (CVD) is the most common cause of death in patients with native and post-transplant chronic kidney disease (CKD). To identify new biomarkers of vascular injury and inflammation, we analyzed the proteome of plasma and circulating extracellular vesicles (EVs) in native...

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Autores principales: Diana Jalal, Bridget Sanford, Brandon Renner, Patrick Ten Eyck, Jennifer Laskowski, James Cooper, Mingyao Sun, Yousef Zakharia, Douglas Spitz, Ayotunde Dokun, Massimo Attanasio, Kenneth Jones, Joshua M. Thurman
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/76de923efa7f4196b52531ea0c40ccba
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spelling oai:doaj.org-article:76de923efa7f4196b52531ea0c40ccba2021-12-02T17:32:58ZDetection of pro angiogenic and inflammatory biomarkers in patients with CKD10.1038/s41598-021-87710-02045-2322https://doaj.org/article/76de923efa7f4196b52531ea0c40ccba2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87710-0https://doaj.org/toc/2045-2322Abstract Cardiovascular disease (CVD) is the most common cause of death in patients with native and post-transplant chronic kidney disease (CKD). To identify new biomarkers of vascular injury and inflammation, we analyzed the proteome of plasma and circulating extracellular vesicles (EVs) in native and post-transplant CKD patients utilizing an aptamer-based assay. Proteins of angiogenesis were significantly higher in native and post-transplant CKD patients versus healthy controls. Ingenuity pathway analysis (IPA) indicated Ephrin receptor signaling, serine biosynthesis, and transforming growth factor-β as the top pathways activated in both CKD groups. Pro-inflammatory proteins were significantly higher only in the EVs of native CKD patients. IPA indicated acute phase response signaling, insulin-like growth factor-1, tumor necrosis factor-α, and interleukin-6 pathway activation. These data indicate that pathways of angiogenesis and inflammation are activated in CKD patients’ plasma and EVs, respectively. The pathways common in both native and post-transplant CKD may signal similar mechanisms of CVD.Diana JalalBridget SanfordBrandon RennerPatrick Ten EyckJennifer LaskowskiJames CooperMingyao SunYousef ZakhariaDouglas SpitzAyotunde DokunMassimo AttanasioKenneth JonesJoshua M. ThurmanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Diana Jalal
Bridget Sanford
Brandon Renner
Patrick Ten Eyck
Jennifer Laskowski
James Cooper
Mingyao Sun
Yousef Zakharia
Douglas Spitz
Ayotunde Dokun
Massimo Attanasio
Kenneth Jones
Joshua M. Thurman
Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
description Abstract Cardiovascular disease (CVD) is the most common cause of death in patients with native and post-transplant chronic kidney disease (CKD). To identify new biomarkers of vascular injury and inflammation, we analyzed the proteome of plasma and circulating extracellular vesicles (EVs) in native and post-transplant CKD patients utilizing an aptamer-based assay. Proteins of angiogenesis were significantly higher in native and post-transplant CKD patients versus healthy controls. Ingenuity pathway analysis (IPA) indicated Ephrin receptor signaling, serine biosynthesis, and transforming growth factor-β as the top pathways activated in both CKD groups. Pro-inflammatory proteins were significantly higher only in the EVs of native CKD patients. IPA indicated acute phase response signaling, insulin-like growth factor-1, tumor necrosis factor-α, and interleukin-6 pathway activation. These data indicate that pathways of angiogenesis and inflammation are activated in CKD patients’ plasma and EVs, respectively. The pathways common in both native and post-transplant CKD may signal similar mechanisms of CVD.
format article
author Diana Jalal
Bridget Sanford
Brandon Renner
Patrick Ten Eyck
Jennifer Laskowski
James Cooper
Mingyao Sun
Yousef Zakharia
Douglas Spitz
Ayotunde Dokun
Massimo Attanasio
Kenneth Jones
Joshua M. Thurman
author_facet Diana Jalal
Bridget Sanford
Brandon Renner
Patrick Ten Eyck
Jennifer Laskowski
James Cooper
Mingyao Sun
Yousef Zakharia
Douglas Spitz
Ayotunde Dokun
Massimo Attanasio
Kenneth Jones
Joshua M. Thurman
author_sort Diana Jalal
title Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
title_short Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
title_full Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
title_fullStr Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
title_full_unstemmed Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
title_sort detection of pro angiogenic and inflammatory biomarkers in patients with ckd
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/76de923efa7f4196b52531ea0c40ccba
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