Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress

Abstract Amino acid transporters have roles in amino acid uptake from soil, long-distance transport, remobilization from vegetative tissues and accumulation in grain. Critically, the majority of wheat grain nitrogen is derived from amino acids remobilized from vegetative organs. However, no systemat...

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Autores principales:	Yongfang Wan, Robert King, Rowan A. C. Mitchell, Keywan Hassani-Pak, Malcolm J. Hawkesford
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76e0af0453ce4bef83e6b2fafb6945a2
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spelling	oai:doaj.org-article:76e0af0453ce4bef83e6b2fafb6945a22021-12-02T16:06:42ZSpatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress10.1038/s41598-017-04473-32045-2322https://doaj.org/article/76e0af0453ce4bef83e6b2fafb6945a22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04473-3https://doaj.org/toc/2045-2322Abstract Amino acid transporters have roles in amino acid uptake from soil, long-distance transport, remobilization from vegetative tissues and accumulation in grain. Critically, the majority of wheat grain nitrogen is derived from amino acids remobilized from vegetative organs. However, no systematic analysis of wheat AAT genes has been reported to date. Here, 283 full length wheat AAT genes representing 100 distinct groups of homeologs were identified and curated by selectively consolidating IWGSC CSSv2 and TGACv1 Triticum aestivum genome assemblies and reassembling or mapping of IWGSC CSS chromosome sorted reads to fill any gaps. Gene expression profiling was performed using public RNA-seq data from root, leaf, stem, spike, grain and grain cells (transfer cell (TC), aleurone cell (AL), and starchy endosperm (SE)). AATs highly expressed in roots are good candidates for amino acid uptake from soil whilst AATs highly expressed in senescing leaves and stems may be involved in translocation to grain. AATs in TC (TaAAP2 and TaAAP19) and SE (TaAAP13) may play important roles in determining grain protein content and grain yield. The expression levels of AAT homeologs showed unequal contributions in response to abiotic stresses and development, which may aid wheat adaptation to a wide range of environments.Yongfang WanRobert KingRowan A. C. MitchellKeywan Hassani-PakMalcolm J. HawkesfordNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongfang Wan Robert King Rowan A. C. Mitchell Keywan Hassani-Pak Malcolm J. Hawkesford Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
description	Abstract Amino acid transporters have roles in amino acid uptake from soil, long-distance transport, remobilization from vegetative tissues and accumulation in grain. Critically, the majority of wheat grain nitrogen is derived from amino acids remobilized from vegetative organs. However, no systematic analysis of wheat AAT genes has been reported to date. Here, 283 full length wheat AAT genes representing 100 distinct groups of homeologs were identified and curated by selectively consolidating IWGSC CSSv2 and TGACv1 Triticum aestivum genome assemblies and reassembling or mapping of IWGSC CSS chromosome sorted reads to fill any gaps. Gene expression profiling was performed using public RNA-seq data from root, leaf, stem, spike, grain and grain cells (transfer cell (TC), aleurone cell (AL), and starchy endosperm (SE)). AATs highly expressed in roots are good candidates for amino acid uptake from soil whilst AATs highly expressed in senescing leaves and stems may be involved in translocation to grain. AATs in TC (TaAAP2 and TaAAP19) and SE (TaAAP13) may play important roles in determining grain protein content and grain yield. The expression levels of AAT homeologs showed unequal contributions in response to abiotic stresses and development, which may aid wheat adaptation to a wide range of environments.
format	article
author	Yongfang Wan Robert King Rowan A. C. Mitchell Keywan Hassani-Pak Malcolm J. Hawkesford
author_facet	Yongfang Wan Robert King Rowan A. C. Mitchell Keywan Hassani-Pak Malcolm J. Hawkesford
author_sort	Yongfang Wan
title	Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
title_short	Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
title_full	Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
title_fullStr	Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
title_full_unstemmed	Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
title_sort	spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/76e0af0453ce4bef83e6b2fafb6945a2
work_keys_str_mv	AT yongfangwan spatiotemporalexpressionpatternsofwheataminoacidtransportersrevealtheirputativerolesinnitrogentransportandresponsestoabioticstress AT robertking spatiotemporalexpressionpatternsofwheataminoacidtransportersrevealtheirputativerolesinnitrogentransportandresponsestoabioticstress AT rowanacmitchell spatiotemporalexpressionpatternsofwheataminoacidtransportersrevealtheirputativerolesinnitrogentransportandresponsestoabioticstress AT keywanhassanipak spatiotemporalexpressionpatternsofwheataminoacidtransportersrevealtheirputativerolesinnitrogentransportandresponsestoabioticstress AT malcolmjhawkesford spatiotemporalexpressionpatternsofwheataminoacidtransportersrevealtheirputativerolesinnitrogentransportandresponsestoabioticstress
_version_	1718384908697600000

Spatiotemporal expression patterns of wheat amino acid transporters reveal their putative roles in nitrogen transport and responses to abiotic stress

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