Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.

A hallmark of the central nervous system is its spatial and functional organization in synaptic layers. During neuronal development, axons form transient contacts with potential post-synaptic elements and establish synapses with appropriate partners at specific layers. These processes are regulated...

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Autores principales:	Sandra Berger-Müller, Atsushi Sugie, Fumio Takahashi, Gaia Tavosanis, Satoko Hakeda-Suzuki, Takashi Suzuki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76e4740b2a2b4d77a870a1080f06662d
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spelling	oai:doaj.org-article:76e4740b2a2b4d77a870a1080f06662d2021-11-18T08:40:19ZAssessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.1932-620310.1371/journal.pone.0083732https://doaj.org/article/76e4740b2a2b4d77a870a1080f06662d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386266/?tool=EBIhttps://doaj.org/toc/1932-6203A hallmark of the central nervous system is its spatial and functional organization in synaptic layers. During neuronal development, axons form transient contacts with potential post-synaptic elements and establish synapses with appropriate partners at specific layers. These processes are regulated by synaptic cell-adhesion molecules. In the Drosophila visual system, R7 and R8 photoreceptor subtypes target distinct layers and form en passant pre-synaptic terminals at stereotypic loci of the axonal shaft. A leucine-rich repeat transmembrane protein, Capricious (Caps), is known to be selectively expressed in R8 axons and their recipient layer, which led to the attractive hypothesis that Caps mediates R8 synaptic specificity by homophilic adhesion. Contradicting this assumption, our results indicate that Caps does not have a prominent role in synaptic-layer targeting and synapse formation in Drosophila photoreceptors, and that the specific recognition of the R8 target layer does not involve Caps homophilic axon-target interactions. We generated flies that express a tagged synaptic marker to evaluate the presence and localization of synapses in R7 and R8 photoreceptors. These genetic tools were used to assess how the synaptic profile is affected when axons are forced to target abnormal layers by expressing axon guidance molecules. When R7 axons were mistargeted to the R8-recipient layer, R7s either maintained an R7-like synaptic profile or acquired a similar profile to r8s depending on the overexpressed protein. When R7 axons were redirected to a more superficial medulla layer, the number of presynaptic terminals was reduced. These results indicate that cell-surface molecules are able to dictate synapse loci by changing the axon terminal identity in a partially cell-autonomous manner, but that presynapse formation at specific sites also requires complex interactions between pre- and post-synaptic elements.Sandra Berger-MüllerAtsushi SugieFumio TakahashiGaia TavosanisSatoko Hakeda-SuzukiTakashi SuzukiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83732 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandra Berger-Müller Atsushi Sugie Fumio Takahashi Gaia Tavosanis Satoko Hakeda-Suzuki Takashi Suzuki Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
description	A hallmark of the central nervous system is its spatial and functional organization in synaptic layers. During neuronal development, axons form transient contacts with potential post-synaptic elements and establish synapses with appropriate partners at specific layers. These processes are regulated by synaptic cell-adhesion molecules. In the Drosophila visual system, R7 and R8 photoreceptor subtypes target distinct layers and form en passant pre-synaptic terminals at stereotypic loci of the axonal shaft. A leucine-rich repeat transmembrane protein, Capricious (Caps), is known to be selectively expressed in R8 axons and their recipient layer, which led to the attractive hypothesis that Caps mediates R8 synaptic specificity by homophilic adhesion. Contradicting this assumption, our results indicate that Caps does not have a prominent role in synaptic-layer targeting and synapse formation in Drosophila photoreceptors, and that the specific recognition of the R8 target layer does not involve Caps homophilic axon-target interactions. We generated flies that express a tagged synaptic marker to evaluate the presence and localization of synapses in R7 and R8 photoreceptors. These genetic tools were used to assess how the synaptic profile is affected when axons are forced to target abnormal layers by expressing axon guidance molecules. When R7 axons were mistargeted to the R8-recipient layer, R7s either maintained an R7-like synaptic profile or acquired a similar profile to r8s depending on the overexpressed protein. When R7 axons were redirected to a more superficial medulla layer, the number of presynaptic terminals was reduced. These results indicate that cell-surface molecules are able to dictate synapse loci by changing the axon terminal identity in a partially cell-autonomous manner, but that presynapse formation at specific sites also requires complex interactions between pre- and post-synaptic elements.
format	article
author	Sandra Berger-Müller Atsushi Sugie Fumio Takahashi Gaia Tavosanis Satoko Hakeda-Suzuki Takashi Suzuki
author_facet	Sandra Berger-Müller Atsushi Sugie Fumio Takahashi Gaia Tavosanis Satoko Hakeda-Suzuki Takashi Suzuki
author_sort	Sandra Berger-Müller
title	Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
title_short	Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
title_full	Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
title_fullStr	Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
title_full_unstemmed	Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.
title_sort	assessing the role of cell-surface molecules in central synaptogenesis in the drosophila visual system.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/76e4740b2a2b4d77a870a1080f06662d
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Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system.

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