Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients

Abstract Liver cirrhosis is often complicated by an immunological imbalance known as cirrhosis-associated immune dysfunction. This study aimed to investigate disturbances in circulating monocytes and dendritic cells in patients with acute decompensation (AD) of cirrhosis. The sample included 39 adul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chandra Chiappin Cardoso, Camila Matiollo, Carolina Hilgert Jacobsen Pereira, Janaina Santana Fonseca, Helder Emmanuel Leite Alves, Otavio Marcos da Silva, Vivian de Souza Menegassi, Claudia Regina dos Santos, Ana Carolina Rabello de Moraes, Leonardo de Lucca Schiavon, Maria Claudia Santos-Silva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76ea579cd717417d82e9ecd7b58f6d17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76ea579cd717417d82e9ecd7b58f6d17
record_format	dspace
spelling	oai:doaj.org-article:76ea579cd717417d82e9ecd7b58f6d172021-12-02T16:31:14ZPatterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients10.1038/s41598-021-85148-y2045-2322https://doaj.org/article/76ea579cd717417d82e9ecd7b58f6d172021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85148-yhttps://doaj.org/toc/2045-2322Abstract Liver cirrhosis is often complicated by an immunological imbalance known as cirrhosis-associated immune dysfunction. This study aimed to investigate disturbances in circulating monocytes and dendritic cells in patients with acute decompensation (AD) of cirrhosis. The sample included 39 adult cirrhotic patients hospitalized for AD, 29 patients with stable cirrhosis (SC), and 30 healthy controls (CTR). Flow cytometry was used to analyze monocyte and dendritic cell subsets in whole blood and quantify cytokines in plasma samples. Cirrhotic groups showed higher frequencies of intermediate monocytes (iMo) than CTR. AD patients had lower percentages of nonclassical monocytes than CTR and SC. Cirrhotic patients had a profound reduction in absolute and relative dendritic cell numbers compared with CTR and showed higher plasmacytoid/classical dendritic cell ratios. Increased plasma levels of IL-6, IL-10, and IL-17A, elevated percentages of CD62L+ monocytes, and reduced HLA-DR expression on classical monocytes (cMo) were also observed in cirrhotic patients. Patients with more advanced liver disease showed increased cMo and reduced tissue macrophages (TiMas) frequencies. It was found that cMo percentages greater than 90.0% within the monocyte compartment and iMo and TiMas percentages lower than 5.7% and 8.6%, respectively, were associated with increased 90-day mortality. Monocytes and dendritic cells are deeply altered in cirrhotic patients, and subset profiles differ between stable and advanced liver disease. High cMo and low TiMas frequencies may be useful biomarkers of disease severity and mortality in liver cirrhosis.Chandra Chiappin CardosoCamila MatiolloCarolina Hilgert Jacobsen PereiraJanaina Santana FonsecaHelder Emmanuel Leite AlvesOtavio Marcos da SilvaVivian de Souza MenegassiClaudia Regina dos SantosAna Carolina Rabello de MoraesLeonardo de Lucca SchiavonMaria Claudia Santos-SilvaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chandra Chiappin Cardoso Camila Matiollo Carolina Hilgert Jacobsen Pereira Janaina Santana Fonseca Helder Emmanuel Leite Alves Otavio Marcos da Silva Vivian de Souza Menegassi Claudia Regina dos Santos Ana Carolina Rabello de Moraes Leonardo de Lucca Schiavon Maria Claudia Santos-Silva Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
description	Abstract Liver cirrhosis is often complicated by an immunological imbalance known as cirrhosis-associated immune dysfunction. This study aimed to investigate disturbances in circulating monocytes and dendritic cells in patients with acute decompensation (AD) of cirrhosis. The sample included 39 adult cirrhotic patients hospitalized for AD, 29 patients with stable cirrhosis (SC), and 30 healthy controls (CTR). Flow cytometry was used to analyze monocyte and dendritic cell subsets in whole blood and quantify cytokines in plasma samples. Cirrhotic groups showed higher frequencies of intermediate monocytes (iMo) than CTR. AD patients had lower percentages of nonclassical monocytes than CTR and SC. Cirrhotic patients had a profound reduction in absolute and relative dendritic cell numbers compared with CTR and showed higher plasmacytoid/classical dendritic cell ratios. Increased plasma levels of IL-6, IL-10, and IL-17A, elevated percentages of CD62L+ monocytes, and reduced HLA-DR expression on classical monocytes (cMo) were also observed in cirrhotic patients. Patients with more advanced liver disease showed increased cMo and reduced tissue macrophages (TiMas) frequencies. It was found that cMo percentages greater than 90.0% within the monocyte compartment and iMo and TiMas percentages lower than 5.7% and 8.6%, respectively, were associated with increased 90-day mortality. Monocytes and dendritic cells are deeply altered in cirrhotic patients, and subset profiles differ between stable and advanced liver disease. High cMo and low TiMas frequencies may be useful biomarkers of disease severity and mortality in liver cirrhosis.
format	article
author	Chandra Chiappin Cardoso Camila Matiollo Carolina Hilgert Jacobsen Pereira Janaina Santana Fonseca Helder Emmanuel Leite Alves Otavio Marcos da Silva Vivian de Souza Menegassi Claudia Regina dos Santos Ana Carolina Rabello de Moraes Leonardo de Lucca Schiavon Maria Claudia Santos-Silva
author_facet	Chandra Chiappin Cardoso Camila Matiollo Carolina Hilgert Jacobsen Pereira Janaina Santana Fonseca Helder Emmanuel Leite Alves Otavio Marcos da Silva Vivian de Souza Menegassi Claudia Regina dos Santos Ana Carolina Rabello de Moraes Leonardo de Lucca Schiavon Maria Claudia Santos-Silva
author_sort	Chandra Chiappin Cardoso
title	Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
title_short	Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
title_full	Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
title_fullStr	Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
title_full_unstemmed	Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
title_sort	patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/76ea579cd717417d82e9ecd7b58f6d17
work_keys_str_mv	AT chandrachiappincardoso patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT camilamatiollo patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT carolinahilgertjacobsenpereira patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT janainasantanafonseca patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT helderemmanuelleitealves patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT otaviomarcosdasilva patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT viviandesouzamenegassi patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT claudiareginadossantos patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT anacarolinarabellodemoraes patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT leonardodeluccaschiavon patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients AT mariaclaudiasantossilva patternsofdendriticcellandmonocytesubsetsareassociatedwithdiseaseseverityandmortalityinlivercirrhosispatients
_version_	1718383882002235392

Patterns of dendritic cell and monocyte subsets are associated with disease severity and mortality in liver cirrhosis patients

Ejemplares similares