Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes

Abstract Sterile male Queensland fruit fly, Bactrocera tryoni (Froggatt), fed as immature adults on the plant compound raspberry ketone (RK), show a reduced attraction to cuelure, a synthetic analogue of RK used as an attractant in Male Annihilation Technique. We hypothesized the reduced attraction...

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Autores principales:	Mohammed Abul Monjur Khan, Nandan P. Deshpande, Lucas A. Shuttleworth, Terry Osborne, Damian Collins, Marc R. Wilkins, Geoff M. Gurr, Olivia L. Reynolds
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76f0429bc4f64789a72821467bd4e617
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spelling	oai:doaj.org-article:76f0429bc4f64789a72821467bd4e6172021-12-02T16:38:49ZRaspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes10.1038/s41598-021-96778-72045-2322https://doaj.org/article/76f0429bc4f64789a72821467bd4e6172021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96778-7https://doaj.org/toc/2045-2322Abstract Sterile male Queensland fruit fly, Bactrocera tryoni (Froggatt), fed as immature adults on the plant compound raspberry ketone (RK), show a reduced attraction to cuelure, a synthetic analogue of RK used as an attractant in Male Annihilation Technique. We hypothesized the reduced attraction of RK-fed adult males to cuelure may be a consequence of altered expression of chemoreception genes. A Y-tube olfactometer assay with RK-fed and RK-unfed sterile B. tryoni males tested the subsequent behavioural response to cuelure. Behavioral assays confirmed a significant decrease in attraction of RK-fed sterile males to cuelure. RK-fed, non-responders (to cue-lure) and RK-unfed, responders (to cue-lure) males were sampled and gene expression compared by de novo RNA-seq analysis. A total of 269 genes in fly heads were differentially expressed between replicated groups of RK-fed, cuelure non-responders and RK-unfed, cuelure responders. Among them, 218 genes including 4 chemoreceptor genes were up regulated and 51 genes were down regulated in RK-fed, cuelure non-responders. De novo assembly generated many genes with unknown functions and no significant BLAST hits to homologues in other species. The enriched and suppressed genes reported here, shed light on the transcriptional changes that affect the dynamics of insect responses to chemical stimuli.Mohammed Abul Monjur KhanNandan P. DeshpandeLucas A. ShuttleworthTerry OsborneDamian CollinsMarc R. WilkinsGeoff M. GurrOlivia L. ReynoldsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammed Abul Monjur Khan Nandan P. Deshpande Lucas A. Shuttleworth Terry Osborne Damian Collins Marc R. Wilkins Geoff M. Gurr Olivia L. Reynolds Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
description	Abstract Sterile male Queensland fruit fly, Bactrocera tryoni (Froggatt), fed as immature adults on the plant compound raspberry ketone (RK), show a reduced attraction to cuelure, a synthetic analogue of RK used as an attractant in Male Annihilation Technique. We hypothesized the reduced attraction of RK-fed adult males to cuelure may be a consequence of altered expression of chemoreception genes. A Y-tube olfactometer assay with RK-fed and RK-unfed sterile B. tryoni males tested the subsequent behavioural response to cuelure. Behavioral assays confirmed a significant decrease in attraction of RK-fed sterile males to cuelure. RK-fed, non-responders (to cue-lure) and RK-unfed, responders (to cue-lure) males were sampled and gene expression compared by de novo RNA-seq analysis. A total of 269 genes in fly heads were differentially expressed between replicated groups of RK-fed, cuelure non-responders and RK-unfed, cuelure responders. Among them, 218 genes including 4 chemoreceptor genes were up regulated and 51 genes were down regulated in RK-fed, cuelure non-responders. De novo assembly generated many genes with unknown functions and no significant BLAST hits to homologues in other species. The enriched and suppressed genes reported here, shed light on the transcriptional changes that affect the dynamics of insect responses to chemical stimuli.
format	article
author	Mohammed Abul Monjur Khan Nandan P. Deshpande Lucas A. Shuttleworth Terry Osborne Damian Collins Marc R. Wilkins Geoff M. Gurr Olivia L. Reynolds
author_facet	Mohammed Abul Monjur Khan Nandan P. Deshpande Lucas A. Shuttleworth Terry Osborne Damian Collins Marc R. Wilkins Geoff M. Gurr Olivia L. Reynolds
author_sort	Mohammed Abul Monjur Khan
title	Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
title_short	Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
title_full	Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
title_fullStr	Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
title_full_unstemmed	Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes
title_sort	raspberry ketone diet supplement reduces attraction of sterile male queensland fruit fly to cuelure by altering expression of chemoreceptor genes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/76f0429bc4f64789a72821467bd4e617
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Raspberry ketone diet supplement reduces attraction of sterile male Queensland fruit fly to cuelure by altering expression of chemoreceptor genes

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