The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei

Abstract In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Renato Graciano de Paula, Amanda Cristina Campos Antoniêto, Cláudia Batista Carraro, Douglas Christian Borges Lopes, Gabriela Felix Persinoti, Nalu Teixeira Aguiar Peres, Nilce Maria Martinez-Rossi, Rafael Silva-Rocha, Roberto Nascimento Silva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Reesei MAPK Signaling Pathway Sugarcane Bagasse MAPK Gene Cellulase Expression Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76f095f353da4a979c38bbdaf7bf2615
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:76f095f353da4a979c38bbdaf7bf2615
record_format	dspace
spelling	oai:doaj.org-article:76f095f353da4a979c38bbdaf7bf26152021-12-02T15:08:13ZThe Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei10.1038/s41598-018-33383-12045-2322https://doaj.org/article/76f095f353da4a979c38bbdaf7bf26152018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33383-1https://doaj.org/toc/2045-2322Abstract In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Δtmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation.Renato Graciano de PaulaAmanda Cristina Campos AntoniêtoCláudia Batista CarraroDouglas Christian Borges LopesGabriela Felix PersinotiNalu Teixeira Aguiar PeresNilce Maria Martinez-RossiRafael Silva-RochaRoberto Nascimento SilvaNature PortfolioarticleReeseiMAPK Signaling PathwaySugarcane BagasseMAPK GeneCellulase ExpressionMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Reesei MAPK Signaling Pathway Sugarcane Bagasse MAPK Gene Cellulase Expression Medicine R Science Q
spellingShingle	Reesei MAPK Signaling Pathway Sugarcane Bagasse MAPK Gene Cellulase Expression Medicine R Science Q Renato Graciano de Paula Amanda Cristina Campos Antoniêto Cláudia Batista Carraro Douglas Christian Borges Lopes Gabriela Felix Persinoti Nalu Teixeira Aguiar Peres Nilce Maria Martinez-Rossi Rafael Silva-Rocha Roberto Nascimento Silva The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
description	Abstract In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Δtmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation.
format	article
author	Renato Graciano de Paula Amanda Cristina Campos Antoniêto Cláudia Batista Carraro Douglas Christian Borges Lopes Gabriela Felix Persinoti Nalu Teixeira Aguiar Peres Nilce Maria Martinez-Rossi Rafael Silva-Rocha Roberto Nascimento Silva
author_facet	Renato Graciano de Paula Amanda Cristina Campos Antoniêto Cláudia Batista Carraro Douglas Christian Borges Lopes Gabriela Felix Persinoti Nalu Teixeira Aguiar Peres Nilce Maria Martinez-Rossi Rafael Silva-Rocha Roberto Nascimento Silva
author_sort	Renato Graciano de Paula
title	The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
title_short	The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
title_full	The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
title_fullStr	The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
title_full_unstemmed	The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
title_sort	duality of the mapk signaling pathway in the control of metabolic processes and cellulase production in trichoderma reesei
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/76f095f353da4a979c38bbdaf7bf2615
work_keys_str_mv	AT renatogracianodepaula thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT amandacristinacamposantonieto thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT claudiabatistacarraro thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT douglaschristianborgeslopes thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT gabrielafelixpersinoti thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT naluteixeiraaguiarperes thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT nilcemariamartinezrossi thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT rafaelsilvarocha thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT robertonascimentosilva thedualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT renatogracianodepaula dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT amandacristinacamposantonieto dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT claudiabatistacarraro dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT douglaschristianborgeslopes dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT gabrielafelixpersinoti dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT naluteixeiraaguiarperes dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT nilcemariamartinezrossi dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT rafaelsilvarocha dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei AT robertonascimentosilva dualityofthemapksignalingpathwayinthecontrolofmetabolicprocessesandcellulaseproductionintrichodermareesei
_version_	1718388259437936640

The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei

Ejemplares similares