High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood

Abstract Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservo...

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Autores principales:	Stefan Petkov, Yonas Bekele, Tadepally Lakshmikanth, Bo Hejdeman, Maurizio Zazzi, Petter Brodin, Francesca Chiodi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76f179641df84fa3977060e2302877a8
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spelling	oai:doaj.org-article:76f179641df84fa3977060e2302877a82021-12-02T15:10:05ZHigh CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood10.1038/s41598-020-77433-z2045-2322https://doaj.org/article/76f179641df84fa3977060e2302877a82020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77433-zhttps://doaj.org/toc/2045-2322Abstract Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45high T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45high cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45high T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45high T cells may contribute to their exhaustion.Stefan PetkovYonas BekeleTadepally LakshmikanthBo HejdemanMaurizio ZazziPetter BrodinFrancesca ChiodiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Petkov Yonas Bekele Tadepally Lakshmikanth Bo Hejdeman Maurizio Zazzi Petter Brodin Francesca Chiodi High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
description	Abstract Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45high T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45high cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45high T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45high T cells may contribute to their exhaustion.
format	article
author	Stefan Petkov Yonas Bekele Tadepally Lakshmikanth Bo Hejdeman Maurizio Zazzi Petter Brodin Francesca Chiodi
author_facet	Stefan Petkov Yonas Bekele Tadepally Lakshmikanth Bo Hejdeman Maurizio Zazzi Petter Brodin Francesca Chiodi
author_sort	Stefan Petkov
title	High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
title_short	High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
title_full	High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
title_fullStr	High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
title_full_unstemmed	High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
title_sort	high cd45 expression of cd8+ and cd4+ t cells correlates with the size of hiv-1 reservoir in blood
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/76f179641df84fa3977060e2302877a8
work_keys_str_mv	AT stefanpetkov highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT yonasbekele highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT tadepallylakshmikanth highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT bohejdeman highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT mauriziozazzi highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT petterbrodin highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood AT francescachiodi highcd45expressionofcd8andcd4tcellscorrelateswiththesizeofhiv1reservoirinblood
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High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood

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