Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study

Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva a...

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Autores principales:	Tilen Senicar, Andraz Kukovicic, Valerija Tkalec, Aleksander Mahnic, Jernej Dolinsek, Maja Rupnik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gluten-degrading microorganisms <i>Veillonella</i> <i>Candida</i> short-chain fatty acids celiac disease microbiota Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/76f2ba18e9a3417f85f5a75f189224cd
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spelling	oai:doaj.org-article:76f2ba18e9a3417f85f5a75f189224cd2021-11-25T18:25:31ZComparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study10.3390/microorganisms91123752076-2607https://doaj.org/article/76f2ba18e9a3417f85f5a75f189224cd2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2375https://doaj.org/toc/2076-2607Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including <i>Candida albicans</i> and <i>Veillonella</i>. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7–46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from <i>Lactobacillus</i> and <i>Enterococcus</i>. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated.Tilen SenicarAndraz KukovicicValerija TkalecAleksander MahnicJernej DolinsekMaja RupnikMDPI AGarticlegluten-degrading microorganisms<i>Veillonella</i><i>Candida</i>short-chain fatty acidsceliac diseasemicrobiotaBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2375, p 2375 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gluten-degrading microorganisms <i>Veillonella</i> <i>Candida</i> short-chain fatty acids celiac disease microbiota Biology (General) QH301-705.5
spellingShingle	gluten-degrading microorganisms <i>Veillonella</i> <i>Candida</i> short-chain fatty acids celiac disease microbiota Biology (General) QH301-705.5 Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
description	Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including <i>Candida albicans</i> and <i>Veillonella</i>. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7–46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from <i>Lactobacillus</i> and <i>Enterococcus</i>. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated.
format	article
author	Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik
author_facet	Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik
author_sort	Tilen Senicar
title	Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
title_short	Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
title_full	Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
title_fullStr	Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
title_full_unstemmed	Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
title_sort	comparison of microbial populations in saliva and feces from healthy and celiac adolescents with conventional and molecular approaches after cultivation on gluten-containing media: an exploratory study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/76f2ba18e9a3417f85f5a75f189224cd
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Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study

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