Short-term azithromycin treatment promotes cornea allograft survival in the rat.

<h4>Background</h4>Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats.<h4>Methods<...

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Autores principales:	Katrin Wacker, Sophy Denker, Antonia Hildebrand, Philipp Eberwein, Thomas Reinhard, Johannes Schwartzkopff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76f46014122a4dacb8a83c91311ff91e
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spelling	oai:doaj.org-article:76f46014122a4dacb8a83c91311ff91e2021-11-18T08:42:51ZShort-term azithromycin treatment promotes cornea allograft survival in the rat.1932-620310.1371/journal.pone.0082687https://doaj.org/article/76f46014122a4dacb8a83c91311ff91e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349336/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats.<h4>Methods</h4>Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry.<h4>Results</h4>AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls.<h4>Conclusions</h4>Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans.Katrin WackerSophy DenkerAntonia HildebrandPhilipp EberweinThomas ReinhardJohannes SchwartzkopffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82687 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrin Wacker Sophy Denker Antonia Hildebrand Philipp Eberwein Thomas Reinhard Johannes Schwartzkopff Short-term azithromycin treatment promotes cornea allograft survival in the rat.
description	<h4>Background</h4>Any inflammatory response following corneal transplantation may induce rejection and irreversible graft failure. The purpose of this study is to analyze the anti-inflammatory effect of azithromycin (AZM) following experimental keratoplasty in rats.<h4>Methods</h4>Corneal transplants were performed between Fisher-donor and Lewis-recipient rats. Recipients were postoperatively treated three times daily with AZM, miglyol, ofloxacin or dexamethasone eye drops. As an additional control, AZM was applied following syngeneic keratoplasty. Furthermore, short-term treatments with AZM for seven days perioperatively or with AZM only three days prior to the transplantation were compared to appropriate controls. All transplants were monitored clinically for opacity, edema, and vascularization. Infiltrating CD45(+), CD4(+), CD8(+), CD25(+), CD161(+) and CD163(+) cells were quantified via immunohistochemistry.<h4>Results</h4>AZM significantly promoted corneal graft survival compared with miglyol or ofloxacin treatment. This effect was comparable to topical dexamethasone. No adverse AZM effect was observed. Histology confirmed a significant reduction of infiltrating leukocytes. The short-term application of AZM for three days prior to transplantation or for seven days perioperatively reduced corneal graft rejection significantly compared with the controls.<h4>Conclusions</h4>Along with antibiotic properties, topical AZM has a strong anti-inflammatory effect. Following keratoplasty, this effect is comparable to topical dexamethasone without the risk of steroid-induced adverse effects. Short-term treatment with AZM three days prior to the transplantation was sufficient to promote graft survival in the rat keratoplasty model. We therefore suggest further assessing the anti-inflammatory function of topical AZM following keratoplasty in humans.
format	article
author	Katrin Wacker Sophy Denker Antonia Hildebrand Philipp Eberwein Thomas Reinhard Johannes Schwartzkopff
author_facet	Katrin Wacker Sophy Denker Antonia Hildebrand Philipp Eberwein Thomas Reinhard Johannes Schwartzkopff
author_sort	Katrin Wacker
title	Short-term azithromycin treatment promotes cornea allograft survival in the rat.
title_short	Short-term azithromycin treatment promotes cornea allograft survival in the rat.
title_full	Short-term azithromycin treatment promotes cornea allograft survival in the rat.
title_fullStr	Short-term azithromycin treatment promotes cornea allograft survival in the rat.
title_full_unstemmed	Short-term azithromycin treatment promotes cornea allograft survival in the rat.
title_sort	short-term azithromycin treatment promotes cornea allograft survival in the rat.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/76f46014122a4dacb8a83c91311ff91e
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Short-term azithromycin treatment promotes cornea allograft survival in the rat.

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