Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.

Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These t...

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Autores principales:	Ryan C Pink, Christopher H Eskiw, Daniel P Caley, David R F Carter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/76fde2f4faae435883e7836778ff693a
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spelling	oai:doaj.org-article:76fde2f4faae435883e7836778ff693a2021-11-18T07:03:50ZAnalysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.1932-620310.1371/journal.pone.0013045https://doaj.org/article/76fde2f4faae435883e7836778ff693a2010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20927371/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These techniques have been applied to the β-globin locus, revealing a complex pattern of interactions with regions along the chromosome that the gene resides on. However, biochemical and microscopy data on the nature of β-globin interactions with other chromosomes is contradictory. Therefore we developed a novel 4C variant, Complete-genome 3C by vectorette amplification (4Cv), which allows an unbiased and quantitative method to examine chromosomal structure. We have used 4Cv to study the microenvironment of the β-globin locus in mice and show that a significant proportion of the interactions of β-globin are inter-chromosomal. Furthermore, our data show that in the liver, where the gene is active, β-globin is more likely to interact with other chromosomes, compared to the brain where the gene is silent and is more likely to interact with other regions along the same chromosome. Our data suggest that transcriptional activation of the β-globin locus leads to a change in nuclear position relative to the chromosome territory.Ryan C PinkRyan C PinkChristopher H EskiwDaniel P CaleyDavid R F CarterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan C Pink Ryan C Pink Christopher H Eskiw Daniel P Caley David R F Carter Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
description	Higher order chromatin folding is critical to a number of developmental processes, including the regulation of gene expression. Recently developed biochemical techniques such as RNA TRAP and chromosome conformation capture (3C) have provided us with the tools to probe chromosomal structures. These techniques have been applied to the β-globin locus, revealing a complex pattern of interactions with regions along the chromosome that the gene resides on. However, biochemical and microscopy data on the nature of β-globin interactions with other chromosomes is contradictory. Therefore we developed a novel 4C variant, Complete-genome 3C by vectorette amplification (4Cv), which allows an unbiased and quantitative method to examine chromosomal structure. We have used 4Cv to study the microenvironment of the β-globin locus in mice and show that a significant proportion of the interactions of β-globin are inter-chromosomal. Furthermore, our data show that in the liver, where the gene is active, β-globin is more likely to interact with other chromosomes, compared to the brain where the gene is silent and is more likely to interact with other regions along the same chromosome. Our data suggest that transcriptional activation of the β-globin locus leads to a change in nuclear position relative to the chromosome territory.
format	article
author	Ryan C Pink Ryan C Pink Christopher H Eskiw Daniel P Caley David R F Carter
author_facet	Ryan C Pink Ryan C Pink Christopher H Eskiw Daniel P Caley David R F Carter
author_sort	Ryan C Pink
title	Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
title_short	Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
title_full	Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
title_fullStr	Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
title_full_unstemmed	Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.
title_sort	analysis of β-globin chromatin micro-environment using a novel 3c variant, 4cv.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/76fde2f4faae435883e7836778ff693a
work_keys_str_mv	AT ryancpink analysisofbglobinchromatinmicroenvironmentusinganovel3cvariant4cv AT ryancpink analysisofbglobinchromatinmicroenvironmentusinganovel3cvariant4cv AT christopherheskiw analysisofbglobinchromatinmicroenvironmentusinganovel3cvariant4cv AT danielpcaley analysisofbglobinchromatinmicroenvironmentusinganovel3cvariant4cv AT davidrfcarter analysisofbglobinchromatinmicroenvironmentusinganovel3cvariant4cv
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Analysis of β-globin chromatin micro-environment using a novel 3C variant, 4Cv.

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