High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt

Abstract Antimicrobial resistance within pets has gained worldwide attention due to pets close contact with humans. This report examined at the molecular level, the antimicrobial resistance mechanisms associated with kennel cough and cat flu. 1378 pets in total were assessed for signs of respiratory...

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Autores principales:	Hazim O. Khalifa, Atef F. Oreiby, Takashi Okanda, Yasuyuki Kato, Tetsuya Matsumoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7707cacd102844b8a5062a709eadfc8a
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spelling	oai:doaj.org-article:7707cacd102844b8a5062a709eadfc8a2021-12-02T14:11:32ZHigh β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt10.1038/s41598-021-82061-22045-2322https://doaj.org/article/7707cacd102844b8a5062a709eadfc8a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82061-2https://doaj.org/toc/2045-2322Abstract Antimicrobial resistance within pets has gained worldwide attention due to pets close contact with humans. This report examined at the molecular level, the antimicrobial resistance mechanisms associated with kennel cough and cat flu. 1378 pets in total were assessed for signs of respiratory infection, and nasal and conjunctival swabs were collected across 76 diseased animals. Phenotypically, 27% of the isolates were characterized by multidrug resistance and possessed high levels of resistance rates to β-lactams. Phenotypic ESBLs/AmpCs production were identified within 40.5% and 24.3% of the isolates, respectively. Genotypically, ESBL- and AmpC-encoding genes were detected in 33.8% and 10.8% of the isolates, respectively, with bla SHV comprising the most identified ESBL, and bla CMY and bla ACT present as the AmpC with the highest levels. qnr genes were identified in 64.9% of the isolates, with qnrS being the most prevalent (44.6%). Several antimicrobial resistance determinants were detected for the first time within pets from Africa, including bla CTX-M-37, bla CTX-M-156, bla SHV-11, bla ACT-23, bla ACT25/31, bla DHA-1, and bla CMY-169. Our results revealed that pets displaying symptoms of respiratory illness are potential sources for pathogenic microbes possessing unique resistance mechanisms which could be disseminated to humans, thus leading to the development of severe untreatable infections in these hosts.Hazim O. KhalifaAtef F. OreibyTakashi OkandaYasuyuki KatoTetsuya MatsumotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hazim O. Khalifa Atef F. Oreiby Takashi Okanda Yasuyuki Kato Tetsuya Matsumoto High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
description	Abstract Antimicrobial resistance within pets has gained worldwide attention due to pets close contact with humans. This report examined at the molecular level, the antimicrobial resistance mechanisms associated with kennel cough and cat flu. 1378 pets in total were assessed for signs of respiratory infection, and nasal and conjunctival swabs were collected across 76 diseased animals. Phenotypically, 27% of the isolates were characterized by multidrug resistance and possessed high levels of resistance rates to β-lactams. Phenotypic ESBLs/AmpCs production were identified within 40.5% and 24.3% of the isolates, respectively. Genotypically, ESBL- and AmpC-encoding genes were detected in 33.8% and 10.8% of the isolates, respectively, with bla SHV comprising the most identified ESBL, and bla CMY and bla ACT present as the AmpC with the highest levels. qnr genes were identified in 64.9% of the isolates, with qnrS being the most prevalent (44.6%). Several antimicrobial resistance determinants were detected for the first time within pets from Africa, including bla CTX-M-37, bla CTX-M-156, bla SHV-11, bla ACT-23, bla ACT25/31, bla DHA-1, and bla CMY-169. Our results revealed that pets displaying symptoms of respiratory illness are potential sources for pathogenic microbes possessing unique resistance mechanisms which could be disseminated to humans, thus leading to the development of severe untreatable infections in these hosts.
format	article
author	Hazim O. Khalifa Atef F. Oreiby Takashi Okanda Yasuyuki Kato Tetsuya Matsumoto
author_facet	Hazim O. Khalifa Atef F. Oreiby Takashi Okanda Yasuyuki Kato Tetsuya Matsumoto
author_sort	Hazim O. Khalifa
title	High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
title_short	High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
title_full	High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
title_fullStr	High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
title_full_unstemmed	High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt
title_sort	high β-lactam resistance in gram-negative bacteria associated with kennel cough and cat flu in egypt
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7707cacd102844b8a5062a709eadfc8a
work_keys_str_mv	AT hazimokhalifa highblactamresistanceingramnegativebacteriaassociatedwithkennelcoughandcatfluinegypt AT atefforeiby highblactamresistanceingramnegativebacteriaassociatedwithkennelcoughandcatfluinegypt AT takashiokanda highblactamresistanceingramnegativebacteriaassociatedwithkennelcoughandcatfluinegypt AT yasuyukikato highblactamresistanceingramnegativebacteriaassociatedwithkennelcoughandcatfluinegypt AT tetsuyamatsumoto highblactamresistanceingramnegativebacteriaassociatedwithkennelcoughandcatfluinegypt
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High β-lactam resistance in Gram-negative bacteria associated with kennel cough and cat flu in Egypt

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