On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.

<h4>Background</h4>Mycobacteria, such as M. leprae and M. tuberculosis infect billions of humans. However, because of appropriate immune responses and antibiotic therapy, overt mycobacterial diseases occur far less frequently. M. avium subspecies paratuberculosis (MAP) causes Johne'...

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Autores principales:	Ramon A Juste, Natalia Elguezabal, Joseba M Garrido, Andres Pavon, Maria V Geijo, Iker Sevilla, Jose-Luis Cabriada, Angel Tejada, Francisco García-Campos, Roberto Casado, Itziar Ochotorena, Ander Izeta, Robert J Greenstein
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/770bbed5b9d6427a879ca70896c5bef5
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spelling	oai:doaj.org-article:770bbed5b9d6427a879ca70896c5bef52021-11-25T06:11:46ZOn the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.1932-620310.1371/journal.pone.0002537https://doaj.org/article/770bbed5b9d6427a879ca70896c5bef52008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18596984/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mycobacteria, such as M. leprae and M. tuberculosis infect billions of humans. However, because of appropriate immune responses and antibiotic therapy, overt mycobacterial diseases occur far less frequently. M. avium subspecies paratuberculosis (MAP) causes Johne's disease in ruminants, an affliction evocative of inflammatory bowel disease (IBD). Several agents used to treat IBD (5-ASA, methotrexate, azathioprine and its metabolite 6-MP) have recently been shown to be antiMAP antibiotics. We herein evaluate the prevalence of MAP DNA in healthy individuals and compare them with IBD patients on antiMAP antibiotics.<h4>Methods</h4>We studied 100 healthy individuals (90 blood donors) and 246 patients with IBD. IS900 MAP DNA was identified using a nested primer PCR in the buffy coat of blood. Positive signal was confirmed as MAP by DNA sequence analysis. PCR positive results frequencies were compared according to medications used. Significance was accepted at p<0.05.<h4>Results</h4>47% (47/100) healthy controls and 16% (40/246) IBD patients were IS900 positive (p<0.0001). MAP DNA was identified in 17% of 143 patients receiving mesalamine and 6% of 16 receiving sulfasalazine. None of the IBD patients receiving methotrexate (n = 9), 6-MP (n = 3), ciprofloxacin (n = 5) or Tacrolimus (n = 3) had MAP DNA detectable in their blood.<h4>Discussion</h4>We found a disquietingly large percentage of healthy individuals have MAP DNA in their blood, the significance of which remains to be determined. Counter-intuitively, the incidence of MAP DNA was significantly lower in patients with IBD. Agents with the most potent in vitro antiMAP activity were associated with clearance of blood MAP DNA. We posit that the use antiMAP antibiotics was responsible for the decreased prevalence of MAP DNA in patients with IBD.Ramon A JusteNatalia ElguezabalJoseba M GarridoAndres PavonMaria V GeijoIker SevillaJose-Luis CabriadaAngel TejadaFrancisco García-CamposRoberto CasadoItziar OchotorenaAnder IzetaRobert J GreensteinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2537 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramon A Juste Natalia Elguezabal Joseba M Garrido Andres Pavon Maria V Geijo Iker Sevilla Jose-Luis Cabriada Angel Tejada Francisco García-Campos Roberto Casado Itziar Ochotorena Ander Izeta Robert J Greenstein On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
description	<h4>Background</h4>Mycobacteria, such as M. leprae and M. tuberculosis infect billions of humans. However, because of appropriate immune responses and antibiotic therapy, overt mycobacterial diseases occur far less frequently. M. avium subspecies paratuberculosis (MAP) causes Johne's disease in ruminants, an affliction evocative of inflammatory bowel disease (IBD). Several agents used to treat IBD (5-ASA, methotrexate, azathioprine and its metabolite 6-MP) have recently been shown to be antiMAP antibiotics. We herein evaluate the prevalence of MAP DNA in healthy individuals and compare them with IBD patients on antiMAP antibiotics.<h4>Methods</h4>We studied 100 healthy individuals (90 blood donors) and 246 patients with IBD. IS900 MAP DNA was identified using a nested primer PCR in the buffy coat of blood. Positive signal was confirmed as MAP by DNA sequence analysis. PCR positive results frequencies were compared according to medications used. Significance was accepted at p<0.05.<h4>Results</h4>47% (47/100) healthy controls and 16% (40/246) IBD patients were IS900 positive (p<0.0001). MAP DNA was identified in 17% of 143 patients receiving mesalamine and 6% of 16 receiving sulfasalazine. None of the IBD patients receiving methotrexate (n = 9), 6-MP (n = 3), ciprofloxacin (n = 5) or Tacrolimus (n = 3) had MAP DNA detectable in their blood.<h4>Discussion</h4>We found a disquietingly large percentage of healthy individuals have MAP DNA in their blood, the significance of which remains to be determined. Counter-intuitively, the incidence of MAP DNA was significantly lower in patients with IBD. Agents with the most potent in vitro antiMAP activity were associated with clearance of blood MAP DNA. We posit that the use antiMAP antibiotics was responsible for the decreased prevalence of MAP DNA in patients with IBD.
format	article
author	Ramon A Juste Natalia Elguezabal Joseba M Garrido Andres Pavon Maria V Geijo Iker Sevilla Jose-Luis Cabriada Angel Tejada Francisco García-Campos Roberto Casado Itziar Ochotorena Ander Izeta Robert J Greenstein
author_facet	Ramon A Juste Natalia Elguezabal Joseba M Garrido Andres Pavon Maria V Geijo Iker Sevilla Jose-Luis Cabriada Angel Tejada Francisco García-Campos Roberto Casado Itziar Ochotorena Ander Izeta Robert J Greenstein
author_sort	Ramon A Juste
title	On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
title_short	On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
title_full	On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
title_fullStr	On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
title_full_unstemmed	On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.
title_sort	on the prevalence of m. avium subspecies paratuberculosis dna in the blood of healthy individuals and patients with inflammatory bowel disease.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/770bbed5b9d6427a879ca70896c5bef5
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On the prevalence of M. avium subspecies paratuberculosis DNA in the blood of healthy individuals and patients with inflammatory bowel disease.

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