Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens
The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and cl...
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2021
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oai:doaj.org-article:77172e251a824a958bf7400b11f503a92021-11-18T08:14:32ZTargeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens1932-6203https://doaj.org/article/77172e251a824a958bf7400b11f503a92021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584957/?tool=EBIhttps://doaj.org/toc/1932-6203The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics.Karel BezstarostiMart M. LamersWouter A. S. DoffPeter C. WeverKhoa T. D. ThaiJeroen J. A. van KampenBart L. HaagmansJeroen A. A. DemmersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Karel Bezstarosti Mart M. Lamers Wouter A. S. Doff Peter C. Wever Khoa T. D. Thai Jeroen J. A. van Kampen Bart L. Haagmans Jeroen A. A. Demmers Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| description |
The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics. |
| format |
article |
| author |
Karel Bezstarosti Mart M. Lamers Wouter A. S. Doff Peter C. Wever Khoa T. D. Thai Jeroen J. A. van Kampen Bart L. Haagmans Jeroen A. A. Demmers |
| author_facet |
Karel Bezstarosti Mart M. Lamers Wouter A. S. Doff Peter C. Wever Khoa T. D. Thai Jeroen J. A. van Kampen Bart L. Haagmans Jeroen A. A. Demmers |
| author_sort |
Karel Bezstarosti |
| title |
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| title_short |
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| title_full |
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| title_fullStr |
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| title_full_unstemmed |
Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |
| title_sort |
targeted proteomics as a tool to detect sars-cov-2 proteins in clinical specimens |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/77172e251a824a958bf7400b11f503a9 |
| work_keys_str_mv |
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1718422050189606912 |