RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.

Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen...

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Autores principales:	Isabelle Derré, Marc Pypaert, Alice Dautry-Varsat, Hervé Agaisse
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7717a8d745e948f4be845b5f2b79583b
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spelling	oai:doaj.org-article:7717a8d745e948f4be845b5f2b79583b2021-11-25T05:46:21ZRNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.1553-73661553-737410.1371/journal.ppat.0030155https://doaj.org/article/7717a8d745e948f4be845b5f2b79583b2007-10-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.0030155https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen and identified 54 factors that, when depleted, inhibit C. caviae infection. By testing the effect of each candidate's knock down on L. monocytogenes infection, we have identified 31 candidates presumably specific of C. caviae infection. We found factors expected to have an effect on Chlamydia infection, such as heparansulfate glycosaminoglycans and actin and microtubule remodeling factors. We also identified factors that were not previously described as involved in Chlamydia infection. For instance, we identified members of the Tim-Tom complex, a multiprotein complex involved in the recognition and import of nuclear-encoded proteins to the mitochondria, as required for C. caviae infection of Drosophila cells. Finally, we confirmed that depletion of either Tom40 or Tom22 also reduced C. caviae infection in mammalian cells. However, C. trachomatis infection was not affected, suggesting that the mechanism involved is C. caviae specific.Isabelle DerréMarc PypaertAlice Dautry-VarsatHervé AgaissePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 3, Iss 10, Pp 1446-1458 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Isabelle Derré Marc Pypaert Alice Dautry-Varsat Hervé Agaisse RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
description	Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen and identified 54 factors that, when depleted, inhibit C. caviae infection. By testing the effect of each candidate's knock down on L. monocytogenes infection, we have identified 31 candidates presumably specific of C. caviae infection. We found factors expected to have an effect on Chlamydia infection, such as heparansulfate glycosaminoglycans and actin and microtubule remodeling factors. We also identified factors that were not previously described as involved in Chlamydia infection. For instance, we identified members of the Tim-Tom complex, a multiprotein complex involved in the recognition and import of nuclear-encoded proteins to the mitochondria, as required for C. caviae infection of Drosophila cells. Finally, we confirmed that depletion of either Tom40 or Tom22 also reduced C. caviae infection in mammalian cells. However, C. trachomatis infection was not affected, suggesting that the mechanism involved is C. caviae specific.
format	article
author	Isabelle Derré Marc Pypaert Alice Dautry-Varsat Hervé Agaisse
author_facet	Isabelle Derré Marc Pypaert Alice Dautry-Varsat Hervé Agaisse
author_sort	Isabelle Derré
title	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
title_short	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
title_full	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
title_fullStr	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
title_full_unstemmed	RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.
title_sort	rnai screen in drosophila cells reveals the involvement of the tom complex in chlamydia infection.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/7717a8d745e948f4be845b5f2b79583b
work_keys_str_mv	AT isabellederre rnaiscreenindrosophilacellsrevealstheinvolvementofthetomcomplexinchlamydiainfection AT marcpypaert rnaiscreenindrosophilacellsrevealstheinvolvementofthetomcomplexinchlamydiainfection AT alicedautryvarsat rnaiscreenindrosophilacellsrevealstheinvolvementofthetomcomplexinchlamydiainfection AT herveagaisse rnaiscreenindrosophilacellsrevealstheinvolvementofthetomcomplexinchlamydiainfection
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RNAi screen in Drosophila cells reveals the involvement of the Tom complex in Chlamydia infection.

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