Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells

Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Chromium oxide nanoparticles (Cr2O3NPs) are widely used in polymers and paints. In the present study, we aimed to determine the toxicity of Cr2O3NPs in murine fib...

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Autores principales:	Alarifi S, Ali D, Alkahtani S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Chromium oxide nanoparticles L929 cells MTT assay Oxidative stress Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/77188181394141adaba9577af40ebbc0
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spelling	oai:doaj.org-article:77188181394141adaba9577af40ebbc02021-12-02T03:11:48ZMechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells1178-2013https://doaj.org/article/77188181394141adaba9577af40ebbc02016-03-01T00:00:00Zhttps://www.dovepress.com/mechanistic-investigation-of-toxicity-of-chromium-oxide-nanoparticles--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Chromium oxide nanoparticles (Cr2O3NPs) are widely used in polymers and paints. In the present study, we aimed to determine the toxicity of Cr2O3NPs in murine fibrosarcoma (L929) cells. The cytotoxicity of Cr2O3NPs was measured by MTT and neutral red uptake assays; Cr2O3NPs had significant cytotoxic effects on L929 cells. Enhancement of intracellular reactive oxygen species was observed in L929 cells after exposure to Cr2O3NPs. Cr2O3NPs produced caspase-3, indicating that exposure to Cr2O3NPs induced apoptosis. After exposure to Cr2O3NPs, the cellular glutathione level decreased and lipid peroxidation, superoxide dismutase, and catalase increased in a dose- and time-dependent manner. By using single-cell gel tests, we also observed increased DNA damage in a Cr2O3NP exposure-duration- and dose-dependent fashion. Cell toxicity and DNA damage may be useful biomarkers for determining the safety of Cr2O3NPs in human and animal health. Keywords: Cr2O3NPs, L929 cells, MTT assay, oxidative stress Alarifi SAli DAlkahtani SDove Medical PressarticleChromium oxide nanoparticlesL929 cellsMTT assayOxidative stressMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1253-1259 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chromium oxide nanoparticles L929 cells MTT assay Oxidative stress Medicine (General) R5-920
spellingShingle	Chromium oxide nanoparticles L929 cells MTT assay Oxidative stress Medicine (General) R5-920 Alarifi S Ali D Alkahtani S Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
description	Saud Alarifi, Daoud Ali, Saad Alkahtani Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia Abstract: Chromium oxide nanoparticles (Cr2O3NPs) are widely used in polymers and paints. In the present study, we aimed to determine the toxicity of Cr2O3NPs in murine fibrosarcoma (L929) cells. The cytotoxicity of Cr2O3NPs was measured by MTT and neutral red uptake assays; Cr2O3NPs had significant cytotoxic effects on L929 cells. Enhancement of intracellular reactive oxygen species was observed in L929 cells after exposure to Cr2O3NPs. Cr2O3NPs produced caspase-3, indicating that exposure to Cr2O3NPs induced apoptosis. After exposure to Cr2O3NPs, the cellular glutathione level decreased and lipid peroxidation, superoxide dismutase, and catalase increased in a dose- and time-dependent manner. By using single-cell gel tests, we also observed increased DNA damage in a Cr2O3NP exposure-duration- and dose-dependent fashion. Cell toxicity and DNA damage may be useful biomarkers for determining the safety of Cr2O3NPs in human and animal health. Keywords: Cr2O3NPs, L929 cells, MTT assay, oxidative stress
format	article
author	Alarifi S Ali D Alkahtani S
author_facet	Alarifi S Ali D Alkahtani S
author_sort	Alarifi S
title	Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
title_short	Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
title_full	Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
title_fullStr	Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
title_full_unstemmed	Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
title_sort	mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/77188181394141adaba9577af40ebbc0
work_keys_str_mv	AT alarifis mechanisticinvestigationoftoxicityofchromiumoxidenanoparticlesinmurinefibrosarcomacells AT alid mechanisticinvestigationoftoxicityofchromiumoxidenanoparticlesinmurinefibrosarcomacells AT alkahtanis mechanisticinvestigationoftoxicityofchromiumoxidenanoparticlesinmurinefibrosarcomacells
_version_	1718401863032766464

Mechanistic investigation of toxicity of chromium oxide nanoparticles in murine fibrosarcoma cells

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