Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer

Background. Nonsmall-cell lung cancer (NSCLC) is the main type of lung cancer, whose morbidity and mortality rank first among malignant tumors. More than 70% of NSCLC patients are diagnosed at locally advanced or advanced stage, missing the best operation period. Chemotherapy and targeted therapy ar...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu Yang, Hongyu Chen, Ruixiao Li, Haoze Li, Xing Rui, Lihong Zhou, Ningning Liu, Qing Ji, Qi Li
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/771ae64dce684c68a8cde9bb7748ea3b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:771ae64dce684c68a8cde9bb7748ea3b
record_format	dspace
spelling	oai:doaj.org-article:771ae64dce684c68a8cde9bb7748ea3b2021-11-15T01:19:25ZMufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer1741-428810.1155/2021/2373865https://doaj.org/article/771ae64dce684c68a8cde9bb7748ea3b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2373865https://doaj.org/toc/1741-4288Background. Nonsmall-cell lung cancer (NSCLC) is the main type of lung cancer, whose morbidity and mortality rank first among malignant tumors. More than 70% of NSCLC patients are diagnosed at locally advanced or advanced stage, missing the best operation period. Chemotherapy and targeted therapy are important means for the treatment of advanced NSCLC, but various side effects seriously affect the curative effect and the life quality of NSCLC patients. Our previous clinical practice has shown that Mufangji Decoction, a classic traditional Chinese medicine, has a significant curative effect in the treatment of NSCLC, but the specific mechanism is not clear. This study intends to explore the potential mechanism of Mufangji Decoction and its active ingredient patchouli alcohol against NSCLC and to provide a scientific basis for the prevention and treatment of NSCLC by traditional Chinese medicine. Methods. The in vivo and in vitro experiments were performed to evaluate the antitumor effects and investigate the underlying mechanism of Mufangji Decoction and its active ingredient patchouli alcohol. Network pharmacology was applied to analyze the effective ingredients and potential targets or signaling pathways of Mufangji Decoction. Results. Our current study shows that Mufangji Decoction can effectively inhibit the growth of subcutaneous transplantation of NSCLC. The following network pharmacological analysis and in vivo experiment suggest that patchouli alcohol is one of the main active ingredients of Mufangji Decoction and exerts antitumor effects. Further mechanism investigation reveals that the antitumor effect of patchouli alcohol is related to the induction of Akt/mTOR signaling pathway-mediated autophagy in NSCLC cells. Conclusion. Mufangji Decoction and its active ingredient patchouli alcohol might exert their antitumor effects in NSCLC partly through regulating Akt/mTOR-mediated autophagy, providing the evidence that traditional Chinese medicine might be a key approach for NSCLC treatment via targeting the Akt/mTOR signal axis.Liu YangHongyu ChenRuixiao LiHaoze LiXing RuiLihong ZhouNingning LiuQing JiQi LiHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Liu Yang Hongyu Chen Ruixiao Li Haoze Li Xing Rui Lihong Zhou Ningning Liu Qing Ji Qi Li Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
description	Background. Nonsmall-cell lung cancer (NSCLC) is the main type of lung cancer, whose morbidity and mortality rank first among malignant tumors. More than 70% of NSCLC patients are diagnosed at locally advanced or advanced stage, missing the best operation period. Chemotherapy and targeted therapy are important means for the treatment of advanced NSCLC, but various side effects seriously affect the curative effect and the life quality of NSCLC patients. Our previous clinical practice has shown that Mufangji Decoction, a classic traditional Chinese medicine, has a significant curative effect in the treatment of NSCLC, but the specific mechanism is not clear. This study intends to explore the potential mechanism of Mufangji Decoction and its active ingredient patchouli alcohol against NSCLC and to provide a scientific basis for the prevention and treatment of NSCLC by traditional Chinese medicine. Methods. The in vivo and in vitro experiments were performed to evaluate the antitumor effects and investigate the underlying mechanism of Mufangji Decoction and its active ingredient patchouli alcohol. Network pharmacology was applied to analyze the effective ingredients and potential targets or signaling pathways of Mufangji Decoction. Results. Our current study shows that Mufangji Decoction can effectively inhibit the growth of subcutaneous transplantation of NSCLC. The following network pharmacological analysis and in vivo experiment suggest that patchouli alcohol is one of the main active ingredients of Mufangji Decoction and exerts antitumor effects. Further mechanism investigation reveals that the antitumor effect of patchouli alcohol is related to the induction of Akt/mTOR signaling pathway-mediated autophagy in NSCLC cells. Conclusion. Mufangji Decoction and its active ingredient patchouli alcohol might exert their antitumor effects in NSCLC partly through regulating Akt/mTOR-mediated autophagy, providing the evidence that traditional Chinese medicine might be a key approach for NSCLC treatment via targeting the Akt/mTOR signal axis.
format	article
author	Liu Yang Hongyu Chen Ruixiao Li Haoze Li Xing Rui Lihong Zhou Ningning Liu Qing Ji Qi Li
author_facet	Liu Yang Hongyu Chen Ruixiao Li Haoze Li Xing Rui Lihong Zhou Ningning Liu Qing Ji Qi Li
author_sort	Liu Yang
title	Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
title_short	Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
title_full	Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
title_fullStr	Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
title_full_unstemmed	Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer
title_sort	mufangji decoction and its active ingredient patchouli alcohol inhibit tumor growth through regulating akt/mtor-mediated autophagy in nonsmall-cell lung cancer
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/771ae64dce684c68a8cde9bb7748ea3b
work_keys_str_mv	AT liuyang mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT hongyuchen mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT ruixiaoli mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT haozeli mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT xingrui mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT lihongzhou mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT ningningliu mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT qingji mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer AT qili mufangjidecoctionanditsactiveingredientpatchoulialcoholinhibittumorgrowththroughregulatingaktmtormediatedautophagyinnonsmallcelllungcancer
_version_	1718428929444806656

Mufangji Decoction and Its Active Ingredient Patchouli Alcohol Inhibit Tumor Growth through Regulating Akt/mTOR-Mediated Autophagy in Nonsmall-Cell Lung Cancer

Ejemplares similares