Analysis of SI-Related BoGAPDH Family Genes and Response of BoGAPC to SI Signal in Brassica oleracea L.

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in Brassica oleracea. In this study, we performed a genome-wide identification of BoG...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qinqin Xie, Hecui Zhang, Dengke Hu, Qianying Liu, Tonghong Zuo, Yizhong Zhang, Yimei Liu, Siru Zhou, Liquan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>B. oleracea</i> L. <i>GAPDH</i> self-incompatibility family expansion <i>GAPC</i> cloning and expression analysis Genetics QH426-470
Acceso en línea:	https://doaj.org/article/771c2fff9631478c97a85b8019cd8400
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:771c2fff9631478c97a85b8019cd8400
record_format	dspace
spelling	oai:doaj.org-article:771c2fff9631478c97a85b8019cd84002021-11-25T17:41:14ZAnalysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.10.3390/genes121117192073-4425https://doaj.org/article/771c2fff9631478c97a85b8019cd84002021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1719https://doaj.org/toc/2073-4425Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in <i>Brassica oleracea</i>. In this study, we performed a genome-wide identification of <i>BoGAPDH</i> in <i>B. oleracea</i> and performed cloning and expression analysis of one of the differentially expressed genes, <i>BoGAPC</i>. A total of 16 members of the <i>BoGAPDH</i> family were identified in <i>B. oleracea</i>, which were conserved, distributed unevenly on chromosomes and had tandem repeat genes. Most of the genes were down-regulated during self-pollination, and the highest expression was found in stigmas and sepals. Different transcriptome data showed that <i>BoGAPDH</i> genes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed gene <i>BoGAPC</i> and found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the stigma, which was consistent with the result of GUS staining. The promoter region of the gene not only has stress response elements and plant hormone response elements, but also has a variety of specific elements for regulating floral organ development. Subcellular localization indicates that the BoGAPC protein is located in the cytoplasm and belongs to the active protein in the cytoplasm. The results of prokaryotic expression showed that the size of the BoGAPC protein was about 37 kDa, which was consistent with the expected results, indicating that the protein was induced in prokaryotic cells. The results of yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted with the BoGAPC protein. The above results suggest that the <i>BoGAPDH</i> family of <i>B. oleracea</i> plays an important role in the process of plant stress resistance, and the <i>BoGAPC</i> gene may be involved in the process of self-incompatibility in <i>B. oleracea</i>, which may respond to SI by encoding proteins directly interacting with SRK.Qinqin XieHecui ZhangDengke HuQianying LiuTonghong ZuoYizhong ZhangYimei LiuSiru ZhouLiquan ZhuMDPI AGarticle<i>B. oleracea</i> L.<i>GAPDH</i>self-incompatibilityfamily expansion<i>GAPC</i>cloning and expression analysisGeneticsQH426-470ENGenes, Vol 12, Iss 1719, p 1719 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>B. oleracea</i> L. <i>GAPDH</i> self-incompatibility family expansion <i>GAPC</i> cloning and expression analysis Genetics QH426-470
spellingShingle	<i>B. oleracea</i> L. <i>GAPDH</i> self-incompatibility family expansion <i>GAPC</i> cloning and expression analysis Genetics QH426-470 Qinqin Xie Hecui Zhang Dengke Hu Qianying Liu Tonghong Zuo Yizhong Zhang Yimei Liu Siru Zhou Liquan Zhu Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
description	Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is not only involved in carbohydrate metabolism, but also plays an important role in stress resistance. However, it has not been reported in <i>Brassica oleracea</i>. In this study, we performed a genome-wide identification of <i>BoGAPDH</i> in <i>B. oleracea</i> and performed cloning and expression analysis of one of the differentially expressed genes, <i>BoGAPC</i>. A total of 16 members of the <i>BoGAPDH</i> family were identified in <i>B. oleracea</i>, which were conserved, distributed unevenly on chromosomes and had tandem repeat genes. Most of the genes were down-regulated during self-pollination, and the highest expression was found in stigmas and sepals. Different transcriptome data showed that <i>BoGAPDH</i> genes were differentially expressed under stress, which was consistent with the results of qRT-PCR. We cloned and analyzed the differentially expressed gene <i>BoGAPC</i> and found that it was in the down-regulated mode 1 h after self-pollination, and the expression was the highest in the stigma, which was consistent with the result of GUS staining. The promoter region of the gene not only has stress response elements and plant hormone response elements, but also has a variety of specific elements for regulating floral organ development. Subcellular localization indicates that the BoGAPC protein is located in the cytoplasm and belongs to the active protein in the cytoplasm. The results of prokaryotic expression showed that the size of the BoGAPC protein was about 37 kDa, which was consistent with the expected results, indicating that the protein was induced in prokaryotic cells. The results of yeast two-hybrid and GST pull-down showed that the SRK kinase domain interacted with the BoGAPC protein. The above results suggest that the <i>BoGAPDH</i> family of <i>B. oleracea</i> plays an important role in the process of plant stress resistance, and the <i>BoGAPC</i> gene may be involved in the process of self-incompatibility in <i>B. oleracea</i>, which may respond to SI by encoding proteins directly interacting with SRK.
format	article
author	Qinqin Xie Hecui Zhang Dengke Hu Qianying Liu Tonghong Zuo Yizhong Zhang Yimei Liu Siru Zhou Liquan Zhu
author_facet	Qinqin Xie Hecui Zhang Dengke Hu Qianying Liu Tonghong Zuo Yizhong Zhang Yimei Liu Siru Zhou Liquan Zhu
author_sort	Qinqin Xie
title	Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
title_short	Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
title_full	Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
title_fullStr	Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
title_full_unstemmed	Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.
title_sort	analysis of si-related <i>bogapdh</i> family genes and response of <i>bogapc</i> to si signal in <i>brassica oleracea</i> l.
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/771c2fff9631478c97a85b8019cd8400
work_keys_str_mv	AT qinqinxie analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT hecuizhang analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT dengkehu analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT qianyingliu analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT tonghongzuo analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT yizhongzhang analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT yimeiliu analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT siruzhou analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail AT liquanzhu analysisofsirelatedibogapdhifamilygenesandresponseofibogapcitosisignalinibrassicaoleraceail
_version_	1718412112016965632

Analysis of SI-Related <i>BoGAPDH</i> Family Genes and Response of <i>BoGAPC</i> to SI Signal in <i>Brassica oleracea</i> L.

Ejemplares similares