Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures
Hao Tang,1 Rongrong Li,2 Huaming Xu,1 Guoping Lu,3 Zhen Liu,1 Wensu Yang,1 Zhaoxin Xia,1 Yi Zhu,1 Jilu Shen1 1The Fourth Affiliated Hospital of Anhui Medical University Laboratory Department, Hefei, People’s Republic of China; 2The First Affiliated Hospital of Anhui Medical University Laboratory Dep...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7729f897cf2d496aaa9d8fb0538bfd20 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7729f897cf2d496aaa9d8fb0538bfd20 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7729f897cf2d496aaa9d8fb0538bfd202021-11-07T18:42:56ZDirect-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures1178-6973https://doaj.org/article/7729f897cf2d496aaa9d8fb0538bfd202021-11-01T00:00:00Zhttps://www.dovepress.com/direct-on-target-microdroplet-growth-assay-for-detection-of-bacterial--peer-reviewed-fulltext-article-IDRhttps://doaj.org/toc/1178-6973Hao Tang,1 Rongrong Li,2 Huaming Xu,1 Guoping Lu,3 Zhen Liu,1 Wensu Yang,1 Zhaoxin Xia,1 Yi Zhu,1 Jilu Shen1 1The Fourth Affiliated Hospital of Anhui Medical University Laboratory Department, Hefei, People’s Republic of China; 2The First Affiliated Hospital of Anhui Medical University Laboratory Department, Hefei, People’s Republic of China; 3Laboratory Department of Fuyang Hospital Affiliated to Anhui Medical University, Fuyang, Anhui, 236000, People’s Republic of ChinaCorrespondence: Jilu ShenThe Fourth Affiliated Hospital of Anhui Medical University Laboratory, No. 100 Huaihai Avenue, Xinzhan District, Hefei, Anhui Province, 230012, People’s Republic of ChinaTel +86 151 5515 2963Email shenjilu@ahmu.edu.cnIntroduction: The recently developed DOT-MGA (direct-on-target microdroplet growth assay) has shown the desirability of direct application of this approach in positive blood cultures and its good performance in detection. This study selected 44 Enterobacteriaceae strains and implemented a DOT-MGA assay on blood cultures to detect their resistance to seven antibiotics. The results of DOT-MGA were compared with the other two antimicrobial susceptibility testing (AST) methods to analyze the detection performance of DOT-MGA.Methods: We adopted the differential centrifugation to process positive blood-culture (BC). Processed BC broth was directly used for rapid AST using DOT-MGA. Droplets of 6 μL with and without antibiotics at the EUCAST breakpoint concentration were spotted in triplicates onto the surface of a MALDI target. The plates were incubated in a wet box for 4 h before the broth was removed with filter paper. Bruker Biotyper software was used to analyze the test results compared with standard database, and the scores were used to quantify and determine the results.Results: DOT-MGA results were compared with the direct-from-BC disk-diffusion method and results were reported by broth microdilution method, respectively. The comparison demonstrated a 100% growth efficiency in DOT-MGA, a 100% classification consistency for ampicillin, ceftriaxone, and gentamicin, and > 93% classification consistency for tobramycin, aztreonam, trimethoprim-sulfamethoxazole (TMP-SMX), and ceftazidime.Discussion: These study results have shown that DOT-MGA is suitable for directly identifying bacterial resistance to positive blood cultures in clinical microbiology laboratories. Furthermore, it is conducive for early diagnosis and treatment of patients with bloodstream infection due to its convenience, time efficiency, and good performance in identifying multiple antibiotic-insensitive bacteria.Keywords: MALDI-TOF, DOT-MGA, rapid antimicrobial susceptibility testing, blood culture, antibiotic resistance, direct-from-BC disk-diffusion methodTang HLi RXu HLu GLiu ZYang WXia ZZhu YShen JDove Medical Pressarticlemaldi-tofdot-mgarapid antimicrobial susceptibility testingblood cultureantibiotic resistancedirect-from-bc disk-diffusion methodInfectious and parasitic diseasesRC109-216ENInfection and Drug Resistance, Vol Volume 14, Pp 4611-4617 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
maldi-tof dot-mga rapid antimicrobial susceptibility testing blood culture antibiotic resistance direct-from-bc disk-diffusion method Infectious and parasitic diseases RC109-216 |
| spellingShingle |
maldi-tof dot-mga rapid antimicrobial susceptibility testing blood culture antibiotic resistance direct-from-bc disk-diffusion method Infectious and parasitic diseases RC109-216 Tang H Li R Xu H Lu G Liu Z Yang W Xia Z Zhu Y Shen J Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| description |
Hao Tang,1 Rongrong Li,2 Huaming Xu,1 Guoping Lu,3 Zhen Liu,1 Wensu Yang,1 Zhaoxin Xia,1 Yi Zhu,1 Jilu Shen1 1The Fourth Affiliated Hospital of Anhui Medical University Laboratory Department, Hefei, People’s Republic of China; 2The First Affiliated Hospital of Anhui Medical University Laboratory Department, Hefei, People’s Republic of China; 3Laboratory Department of Fuyang Hospital Affiliated to Anhui Medical University, Fuyang, Anhui, 236000, People’s Republic of ChinaCorrespondence: Jilu ShenThe Fourth Affiliated Hospital of Anhui Medical University Laboratory, No. 100 Huaihai Avenue, Xinzhan District, Hefei, Anhui Province, 230012, People’s Republic of ChinaTel +86 151 5515 2963Email shenjilu@ahmu.edu.cnIntroduction: The recently developed DOT-MGA (direct-on-target microdroplet growth assay) has shown the desirability of direct application of this approach in positive blood cultures and its good performance in detection. This study selected 44 Enterobacteriaceae strains and implemented a DOT-MGA assay on blood cultures to detect their resistance to seven antibiotics. The results of DOT-MGA were compared with the other two antimicrobial susceptibility testing (AST) methods to analyze the detection performance of DOT-MGA.Methods: We adopted the differential centrifugation to process positive blood-culture (BC). Processed BC broth was directly used for rapid AST using DOT-MGA. Droplets of 6 μL with and without antibiotics at the EUCAST breakpoint concentration were spotted in triplicates onto the surface of a MALDI target. The plates were incubated in a wet box for 4 h before the broth was removed with filter paper. Bruker Biotyper software was used to analyze the test results compared with standard database, and the scores were used to quantify and determine the results.Results: DOT-MGA results were compared with the direct-from-BC disk-diffusion method and results were reported by broth microdilution method, respectively. The comparison demonstrated a 100% growth efficiency in DOT-MGA, a 100% classification consistency for ampicillin, ceftriaxone, and gentamicin, and > 93% classification consistency for tobramycin, aztreonam, trimethoprim-sulfamethoxazole (TMP-SMX), and ceftazidime.Discussion: These study results have shown that DOT-MGA is suitable for directly identifying bacterial resistance to positive blood cultures in clinical microbiology laboratories. Furthermore, it is conducive for early diagnosis and treatment of patients with bloodstream infection due to its convenience, time efficiency, and good performance in identifying multiple antibiotic-insensitive bacteria.Keywords: MALDI-TOF, DOT-MGA, rapid antimicrobial susceptibility testing, blood culture, antibiotic resistance, direct-from-BC disk-diffusion method |
| format |
article |
| author |
Tang H Li R Xu H Lu G Liu Z Yang W Xia Z Zhu Y Shen J |
| author_facet |
Tang H Li R Xu H Lu G Liu Z Yang W Xia Z Zhu Y Shen J |
| author_sort |
Tang H |
| title |
Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| title_short |
Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| title_full |
Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| title_fullStr |
Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| title_full_unstemmed |
Direct-on-Target Microdroplet Growth Assay for Detection of Bacterial Resistance in Positive Blood Cultures |
| title_sort |
direct-on-target microdroplet growth assay for detection of bacterial resistance in positive blood cultures |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/7729f897cf2d496aaa9d8fb0538bfd20 |
| work_keys_str_mv |
AT tangh directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT lir directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT xuh directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT lug directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT liuz directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT yangw directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT xiaz directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT zhuy directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures AT shenj directontargetmicrodropletgrowthassayfordetectionofbacterialresistanceinpositivebloodcultures |
| _version_ |
1718443291509260288 |