Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos

Abstract The 3C protease is a key factor in picornavirus-induced pathologies with a comprehensive action on cell targets. However, the effects induced by the enzyme have not been described at the organismic level. Here, the model of developing Danio rerio embryos was used to analyze possible toxic e...

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Autores principales:	Polina I. Selina, Maria A. Karaseva, Alexey A. Komissarov, Dina R. Safina, Nataliya A. Lunina, Marina P. Roschina, Eugene D. Sverdlov, Ilya V. Demidyuk, Sergey V. Kostrov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/772d83233cec4436b1853b96c6567904
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spelling	oai:doaj.org-article:772d83233cec4436b1853b96c65679042021-12-02T18:33:47ZEmbryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos10.1038/s41598-021-97641-52045-2322https://doaj.org/article/772d83233cec4436b1853b96c65679042021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97641-5https://doaj.org/toc/2045-2322Abstract The 3C protease is a key factor in picornavirus-induced pathologies with a comprehensive action on cell targets. However, the effects induced by the enzyme have not been described at the organismic level. Here, the model of developing Danio rerio embryos was used to analyze possible toxic effects of the 3C protease of human hepatitis A virus (3Cpro) at the whole-body level. The transient 3Cpro expression had a notable lethal effect and induced a number of specific abnormalities in Danio rerio embryos within 24 h. These effects are due to the proteolytic activity of the enzyme. At the same time, the 3Cpro variant with reduced catalytic activity (3Cmut) increased the incidence of embryonic abnormalities; however, this effect was smaller compared to the native enzyme form. While the expression of 3Cmut increased the overall rate of abnormalities, no predominance of specific ones was observed. The data obtained point to a presence significant impact of picornavirus 3Cprotease at the whole-organism level and make contribution to the study of the infectious process caused by human hepatitis A virus.Polina I. SelinaMaria A. KarasevaAlexey A. KomissarovDina R. SafinaNataliya A. LuninaMarina P. RoschinaEugene D. SverdlovIlya V. DemidyukSergey V. KostrovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Polina I. Selina Maria A. Karaseva Alexey A. Komissarov Dina R. Safina Nataliya A. Lunina Marina P. Roschina Eugene D. Sverdlov Ilya V. Demidyuk Sergey V. Kostrov Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
description	Abstract The 3C protease is a key factor in picornavirus-induced pathologies with a comprehensive action on cell targets. However, the effects induced by the enzyme have not been described at the organismic level. Here, the model of developing Danio rerio embryos was used to analyze possible toxic effects of the 3C protease of human hepatitis A virus (3Cpro) at the whole-body level. The transient 3Cpro expression had a notable lethal effect and induced a number of specific abnormalities in Danio rerio embryos within 24 h. These effects are due to the proteolytic activity of the enzyme. At the same time, the 3Cpro variant with reduced catalytic activity (3Cmut) increased the incidence of embryonic abnormalities; however, this effect was smaller compared to the native enzyme form. While the expression of 3Cmut increased the overall rate of abnormalities, no predominance of specific ones was observed. The data obtained point to a presence significant impact of picornavirus 3Cprotease at the whole-organism level and make contribution to the study of the infectious process caused by human hepatitis A virus.
format	article
author	Polina I. Selina Maria A. Karaseva Alexey A. Komissarov Dina R. Safina Nataliya A. Lunina Marina P. Roschina Eugene D. Sverdlov Ilya V. Demidyuk Sergey V. Kostrov
author_facet	Polina I. Selina Maria A. Karaseva Alexey A. Komissarov Dina R. Safina Nataliya A. Lunina Marina P. Roschina Eugene D. Sverdlov Ilya V. Demidyuk Sergey V. Kostrov
author_sort	Polina I. Selina
title	Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
title_short	Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
title_full	Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
title_fullStr	Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
title_full_unstemmed	Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos
title_sort	embryotoxic activity of 3c protease of human hepatitis a virus in developing danio rerio embryos
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/772d83233cec4436b1853b96c6567904
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Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos

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