Identification of ischemic regions in a rat model of stroke.

<h4>Background</h4>Investigations following stroke first of all require information about the spatio-temporal dimension of the ischemic core as well as of perilesional and remote affected tissue. Here we systematically evaluated regions differently impaired by focal ischemia.<h4>Me...

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Autores principales:	Anke Popp, Nadine Jaenisch, Otto W Witte, Christiane Frahm
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7736af1ae1384da0b5e4291bd44f2705
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spelling	oai:doaj.org-article:7736af1ae1384da0b5e4291bd44f27052021-11-25T06:16:48ZIdentification of ischemic regions in a rat model of stroke.1932-620310.1371/journal.pone.0004764https://doaj.org/article/7736af1ae1384da0b5e4291bd44f27052009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19274095/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Investigations following stroke first of all require information about the spatio-temporal dimension of the ischemic core as well as of perilesional and remote affected tissue. Here we systematically evaluated regions differently impaired by focal ischemia.<h4>Methodology/principal findings</h4>Wistar rats underwent a transient 30 or 120 min suture-occlusion of the middle cerebral artery (MCAO) followed by various reperfusion times (2 h, 1 d, 7 d, 30 d) or a permanent MCAO (1 d survival). Brains were characterized by TTC, thionine, and immunohistochemistry using MAP2, HSP72, and HSP27. TTC staining reliably identifies the infarct core at 1 d of reperfusion after 30 min MCAO and at all investigated times following 120 min and permanent MCAO. Nissl histology denotes the infarct core from 2 h up to 30 d after transient as well as permanent MCAO. Absent and attenuated MAP2 staining clearly identifies the infarct core and perilesional affected regions at all investigated times, respectively. HSP72 denotes perilesional areas in a limited post-ischemic time (1 d). HSP27 detects perilesional and remote impaired tissue from post-ischemic day 1 on. Furthermore a simultaneous expression of HSP72 and HSP27 in perilesional neurons was revealed.<h4>Conclusions/significance</h4>TTC and Nissl staining can be applied to designate the infarct core. MAP2, HSP72, and HSP27 are excellent markers not only to identify perilesional and remote areas but also to discriminate affected neuronal and glial populations. Moreover markers vary in their confinement to different reperfusion times. The extent and consistency of infarcts increase with prolonged occlusion of the MCA. Therefore interindividual infarct dimension should be precisely assessed by the combined use of different markers as described in this study.Anke PoppNadine JaenischOtto W WitteChristiane FrahmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4764 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anke Popp Nadine Jaenisch Otto W Witte Christiane Frahm Identification of ischemic regions in a rat model of stroke.
description	<h4>Background</h4>Investigations following stroke first of all require information about the spatio-temporal dimension of the ischemic core as well as of perilesional and remote affected tissue. Here we systematically evaluated regions differently impaired by focal ischemia.<h4>Methodology/principal findings</h4>Wistar rats underwent a transient 30 or 120 min suture-occlusion of the middle cerebral artery (MCAO) followed by various reperfusion times (2 h, 1 d, 7 d, 30 d) or a permanent MCAO (1 d survival). Brains were characterized by TTC, thionine, and immunohistochemistry using MAP2, HSP72, and HSP27. TTC staining reliably identifies the infarct core at 1 d of reperfusion after 30 min MCAO and at all investigated times following 120 min and permanent MCAO. Nissl histology denotes the infarct core from 2 h up to 30 d after transient as well as permanent MCAO. Absent and attenuated MAP2 staining clearly identifies the infarct core and perilesional affected regions at all investigated times, respectively. HSP72 denotes perilesional areas in a limited post-ischemic time (1 d). HSP27 detects perilesional and remote impaired tissue from post-ischemic day 1 on. Furthermore a simultaneous expression of HSP72 and HSP27 in perilesional neurons was revealed.<h4>Conclusions/significance</h4>TTC and Nissl staining can be applied to designate the infarct core. MAP2, HSP72, and HSP27 are excellent markers not only to identify perilesional and remote areas but also to discriminate affected neuronal and glial populations. Moreover markers vary in their confinement to different reperfusion times. The extent and consistency of infarcts increase with prolonged occlusion of the MCA. Therefore interindividual infarct dimension should be precisely assessed by the combined use of different markers as described in this study.
format	article
author	Anke Popp Nadine Jaenisch Otto W Witte Christiane Frahm
author_facet	Anke Popp Nadine Jaenisch Otto W Witte Christiane Frahm
author_sort	Anke Popp
title	Identification of ischemic regions in a rat model of stroke.
title_short	Identification of ischemic regions in a rat model of stroke.
title_full	Identification of ischemic regions in a rat model of stroke.
title_fullStr	Identification of ischemic regions in a rat model of stroke.
title_full_unstemmed	Identification of ischemic regions in a rat model of stroke.
title_sort	identification of ischemic regions in a rat model of stroke.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/7736af1ae1384da0b5e4291bd44f2705
work_keys_str_mv	AT ankepopp identificationofischemicregionsinaratmodelofstroke AT nadinejaenisch identificationofischemicregionsinaratmodelofstroke AT ottowwitte identificationofischemicregionsinaratmodelofstroke AT christianefrahm identificationofischemicregionsinaratmodelofstroke
_version_	1718413991497170944

Identification of ischemic regions in a rat model of stroke.

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