Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains

Abstract High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the microbial community associated with the different stages of the life-cycle of ovine Haemonchus contortus field strains usi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saeed El-Ashram, Xun Suo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/773fd6994e8f404d9609408c4545d76e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:773fd6994e8f404d9609408c4545d76e
record_format	dspace
spelling	oai:doaj.org-article:773fd6994e8f404d9609408c4545d76e2021-12-02T11:40:14ZExploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains10.1038/s41598-017-00171-22045-2322https://doaj.org/article/773fd6994e8f404d9609408c4545d76e2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00171-2https://doaj.org/toc/2045-2322Abstract High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the microbial community associated with the different stages of the life-cycle of ovine Haemonchus contortus field strains using two distinct amplification primer sets (targeting V3–V4, and V5–V7). Scanning electron microscope and polymerase chain reaction coupled with Illumina MiSeq platform were employed to confirm the absence of any parasite surface contamination by intact bacteria or their DNA products. Results showed 48 (V3–V4 tags) and 28 (V5–V7 tags) bacterial genera comprised the microbial flora of H. contortus microbiome. The dominant bacterial genera belonged to Escherichia-Shigella, Pseudomonas and Ochrobactrum, which were shared in all the stages of the parasite life-cycle using V3–V4 and V5–V7 amplicons. Moreover, the parasite microbiome could reflect the external micro-organisms (i.e. micro- and macro-habitats). There is abundant room for further progress in comparing microbiome of different helminths, which has, and will continue to offer considerable potential for better understanding a wide-variety of devastating animal and human diseases.Saeed El-AshramXun SuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saeed El-Ashram Xun Suo Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
description	Abstract High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the microbial community associated with the different stages of the life-cycle of ovine Haemonchus contortus field strains using two distinct amplification primer sets (targeting V3–V4, and V5–V7). Scanning electron microscope and polymerase chain reaction coupled with Illumina MiSeq platform were employed to confirm the absence of any parasite surface contamination by intact bacteria or their DNA products. Results showed 48 (V3–V4 tags) and 28 (V5–V7 tags) bacterial genera comprised the microbial flora of H. contortus microbiome. The dominant bacterial genera belonged to Escherichia-Shigella, Pseudomonas and Ochrobactrum, which were shared in all the stages of the parasite life-cycle using V3–V4 and V5–V7 amplicons. Moreover, the parasite microbiome could reflect the external micro-organisms (i.e. micro- and macro-habitats). There is abundant room for further progress in comparing microbiome of different helminths, which has, and will continue to offer considerable potential for better understanding a wide-variety of devastating animal and human diseases.
format	article
author	Saeed El-Ashram Xun Suo
author_facet	Saeed El-Ashram Xun Suo
author_sort	Saeed El-Ashram
title	Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
title_short	Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
title_full	Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
title_fullStr	Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
title_full_unstemmed	Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains
title_sort	exploring the microbial community (microflora) associated with ovine haemonchus contortus (macroflora) field strains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/773fd6994e8f404d9609408c4545d76e
work_keys_str_mv	AT saeedelashram exploringthemicrobialcommunitymicrofloraassociatedwithovinehaemonchuscontortusmacroflorafieldstrains AT xunsuo exploringthemicrobialcommunitymicrofloraassociatedwithovinehaemonchuscontortusmacroflorafieldstrains
_version_	1718395653123473408

Exploring the microbial community (microflora) associated with ovine Haemonchus contortus (macroflora) field strains

Ejemplares similares