Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling

Collagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report...

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Autores principales:	Yoanna Ariosa-Morejon, Alberto Santos, Roman Fischer, Simon Davis, Philip Charles, Rajesh Thakker, Angus KT Wann, Tonia L Vincent
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Age protein dynamics proteomics SILAC matrisome collagen-rich tissues Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7742f5d2092644ec824ba5a576a98084
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id	oai:doaj.org-article:7742f5d2092644ec824ba5a576a98084
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spelling	oai:doaj.org-article:7742f5d2092644ec824ba5a576a980842021-11-15T06:43:56ZAge-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling10.7554/eLife.666352050-084Xe66635https://doaj.org/article/7742f5d2092644ec824ba5a576a980842021-09-01T00:00:00Zhttps://elifesciences.org/articles/66635https://doaj.org/toc/2050-084XCollagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report dynamic turnover of the matrisome, the proteins of the extracellular matrix, in bone and cartilage during skeletal maturation, which was markedly reduced after skeletal maturity. Comparing young adult with older adult mice, new protein incorporation was reduced in all tissues. STRING clustering revealed changes in epigenetic modulators across all tissues, a decline in chondroprotective growth factors such as FGF2 and TGFβ in cartilage, and clusters indicating mitochondrial dysregulation and reduced collagen synthesis in bone. Several pathways were implicated in age-related disease. Fewer changes were observed for skin. This methodology provides dynamic protein data at a tissue level, uncovering age-related molecular changes that may predispose to disease.Yoanna Ariosa-MorejonAlberto SantosRoman FischerSimon DavisPhilip CharlesRajesh ThakkerAngus KT WannTonia L VincenteLife Sciences Publications LtdarticleAgeprotein dynamicsproteomicsSILACmatrisomecollagen-rich tissuesMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Age protein dynamics proteomics SILAC matrisome collagen-rich tissues Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Age protein dynamics proteomics SILAC matrisome collagen-rich tissues Medicine R Science Q Biology (General) QH301-705.5 Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
description	Collagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report dynamic turnover of the matrisome, the proteins of the extracellular matrix, in bone and cartilage during skeletal maturation, which was markedly reduced after skeletal maturity. Comparing young adult with older adult mice, new protein incorporation was reduced in all tissues. STRING clustering revealed changes in epigenetic modulators across all tissues, a decline in chondroprotective growth factors such as FGF2 and TGFβ in cartilage, and clusters indicating mitochondrial dysregulation and reduced collagen synthesis in bone. Several pathways were implicated in age-related disease. Fewer changes were observed for skin. This methodology provides dynamic protein data at a tissue level, uncovering age-related molecular changes that may predispose to disease.
format	article
author	Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent
author_facet	Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent
author_sort	Yoanna Ariosa-Morejon
title	Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
title_short	Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
title_full	Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
title_fullStr	Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
title_full_unstemmed	Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
title_sort	age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed silac labelling
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/7742f5d2092644ec824ba5a576a98084
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Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling

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