Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation

Background and Aims. Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. This study aimed to examine the feasibility of cryopreserving dental pulp tis...

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Autores principales:	Ming Yan, Ola A. Nada, Ralf Smeets, Martin Gosau, Reinhard E. Friedrich, Lan Kluwe
Formato:	article
Lenguaje:	EN
Publicado:	Palacký University Olomouc, Faculty of Medicine and Dentistry 2021
Materias:	dental pulp cells differentiation dental pulp tissue cell culture tissue cryopreservation osteogenic differentiation adipogenic differentiation Medicine R
Acceso en línea:	https://doaj.org/article/7745f9ddf8884cb9a5e59605657b1408
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spelling	oai:doaj.org-article:7745f9ddf8884cb9a5e59605657b14082021-11-29T09:17:21ZCompare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation1213-81181804-7521https://doaj.org/article/7745f9ddf8884cb9a5e59605657b14082021-11-01T00:00:00Zhttps://biomed.papers.upol.cz/artkey/bio-202104-0015_compare-features-of-human-dental-pulp-cells-cultured-from-pulp-tissues-with-and-without-cryopreservation.phphttps://doaj.org/toc/1213-8118https://doaj.org/toc/1804-7521Background and Aims. Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. This study aimed to examine the feasibility of cryopreserving dental pulp tissue at teeth extraction for later culturing of cells. Methods. The pulp tissue from each of a total of 10 teeth was cut into small fragments which were then divided into two portions. One portion was directly used for culturing pulp cells using the explant method. The other portion was cryopreserved with 10% DMSO in liquid nitrogen for at least one month and then thawed for culturing pulp cells. Results. Vital cells were obtained from all the 10 pulp fragment suspensions which went through cryopreservation. The cell outgrowth from the explants of cryopreserved pulp fragments was two days later than that of corresponding fresh pulp tissue. Otherwise, no difference was observed in proliferation, expression of stem cell markers and differentiation into adipose cells and osteoblasts between the two groups of cells cultured from the fresh or the cryopreserved pulp fragments. Conclusions. Cryopreserving fragmented dental pulp tissue provides a feasible option for saving pulp tissues as autologous cell sources for possible later application.Ming YanOla A. NadaRalf SmeetsMartin GosauReinhard E. FriedrichLan KluwePalacký University Olomouc, Faculty of Medicine and Dentistryarticledental pulp cellsdifferentiationdental pulp tissuecell culturetissue cryopreservationosteogenic differentiationadipogenic differentiationMedicineRENBiomedical Papers, Vol 165, Iss 4, Pp 445-451 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental pulp cells differentiation dental pulp tissue cell culture tissue cryopreservation osteogenic differentiation adipogenic differentiation Medicine R
spellingShingle	dental pulp cells differentiation dental pulp tissue cell culture tissue cryopreservation osteogenic differentiation adipogenic differentiation Medicine R Ming Yan Ola A. Nada Ralf Smeets Martin Gosau Reinhard E. Friedrich Lan Kluwe Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
description	Background and Aims. Teeth extracted are usually disposed as bio-waste whereas they could serve as an autologous tissue for culturing multipotent dental pulp cells which have application potential in regenerative medicine. This study aimed to examine the feasibility of cryopreserving dental pulp tissue at teeth extraction for later culturing of cells. Methods. The pulp tissue from each of a total of 10 teeth was cut into small fragments which were then divided into two portions. One portion was directly used for culturing pulp cells using the explant method. The other portion was cryopreserved with 10% DMSO in liquid nitrogen for at least one month and then thawed for culturing pulp cells. Results. Vital cells were obtained from all the 10 pulp fragment suspensions which went through cryopreservation. The cell outgrowth from the explants of cryopreserved pulp fragments was two days later than that of corresponding fresh pulp tissue. Otherwise, no difference was observed in proliferation, expression of stem cell markers and differentiation into adipose cells and osteoblasts between the two groups of cells cultured from the fresh or the cryopreserved pulp fragments. Conclusions. Cryopreserving fragmented dental pulp tissue provides a feasible option for saving pulp tissues as autologous cell sources for possible later application.
format	article
author	Ming Yan Ola A. Nada Ralf Smeets Martin Gosau Reinhard E. Friedrich Lan Kluwe
author_facet	Ming Yan Ola A. Nada Ralf Smeets Martin Gosau Reinhard E. Friedrich Lan Kluwe
author_sort	Ming Yan
title	Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
title_short	Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
title_full	Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
title_fullStr	Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
title_full_unstemmed	Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
title_sort	compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation
publisher	Palacký University Olomouc, Faculty of Medicine and Dentistry
publishDate	2021
url	https://doaj.org/article/7745f9ddf8884cb9a5e59605657b1408
work_keys_str_mv	AT mingyan comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation AT olaanada comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation AT ralfsmeets comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation AT martingosau comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation AT reinhardefriedrich comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation AT lankluwe comparefeaturesofhumandentalpulpcellsculturedfrompulptissueswithandwithoutcryopreservation
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Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation

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