Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage

ABSTRACT Resistance to last-line polymyxins mediated by the plasmid-borne mobile colistin resistance gene (mcr-1) represents a new threat to global human health. Here we present the complete genome sequence of an mcr-1-positive multidrug-resistant Escherichia coli strain (MS8345). We show that MS834...

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Autores principales:	Brian M. Forde, Hosam M. Zowawi, Patrick N. A. Harris, Leah Roberts, Emad Ibrahim, Nissar Shaikh, Anand Deshmukh, Mazen A. Sid Ahmed, Muna Al Maslamani, Kyra Cottrell, Ella Trembizki, Lana Sundac, Heidi H. Yu, Jian Li, Mark A. Schembri, David M. Whiley, David L. Paterson, Scott A. Beatson
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Escherichia coli antibiotic resistance genome analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/774be1daa8cb4e5d8b8f79dd7eb81405
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spelling	oai:doaj.org-article:774be1daa8cb4e5d8b8f79dd7eb814052021-11-15T15:22:26ZDiscovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage10.1128/mSphere.00486-182379-5042https://doaj.org/article/774be1daa8cb4e5d8b8f79dd7eb814052018-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00486-18https://doaj.org/toc/2379-5042ABSTRACT Resistance to last-line polymyxins mediated by the plasmid-borne mobile colistin resistance gene (mcr-1) represents a new threat to global human health. Here we present the complete genome sequence of an mcr-1-positive multidrug-resistant Escherichia coli strain (MS8345). We show that MS8345 belongs to serotype O2:K1:H4, has a large 241,164-bp IncHI2 plasmid that carries 15 other antibiotic resistance genes (including the extended-spectrum β-lactamase blaCTX-M-1) and 3 putative multidrug efflux systems, and contains 14 chromosomally encoded antibiotic resistance genes. MS8345 also carries a large ColV-like virulence plasmid that has been associated with E. coli bacteremia. Whole-genome phylogeny revealed that MS8345 clusters within a discrete clade in the sequence type 95 (ST95) lineage, and MS8345 is very closely related to the highly virulent O45:K1:H4 clone associated with neonatal meningitis. Overall, the acquisition of a plasmid carrying resistance to colistin and multiple other antibiotics in this virulent E. coli lineage is concerning and might herald an era where the empirical treatment of ST95 infections becomes increasingly more difficult. IMPORTANCE Escherichia coli ST95 is a globally disseminated clone frequently associated with bloodstream infections and neonatal meningitis. However, the ST95 lineage is defined by low levels of drug resistance amongst clinical isolates, which normally provides for uncomplicated treatment options. Here, we provide the first detailed genomic analysis of an E. coli ST95 isolate that has both high virulence potential and resistance to multiple antibiotics. Using the genome, we predicted its virulence and antibiotic resistance mechanisms, which include resistance to last-line antibiotics mediated by the plasmid-borne mcr-1 gene. Finding an ST95 isolate resistant to nearly all antibiotics that also has a high virulence potential is of major clinical importance and underscores the need to monitor new and emerging trends in antibiotic resistance development in this important global lineage.Brian M. FordeHosam M. ZowawiPatrick N. A. HarrisLeah RobertsEmad IbrahimNissar ShaikhAnand DeshmukhMazen A. Sid AhmedMuna Al MaslamaniKyra CottrellElla TrembizkiLana SundacHeidi H. YuJian LiMark A. SchembriDavid M. WhileyDavid L. PatersonScott A. BeatsonAmerican Society for MicrobiologyarticleEscherichia coliantibiotic resistancegenome analysisMicrobiologyQR1-502ENmSphere, Vol 3, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli antibiotic resistance genome analysis Microbiology QR1-502
spellingShingle	Escherichia coli antibiotic resistance genome analysis Microbiology QR1-502 Brian M. Forde Hosam M. Zowawi Patrick N. A. Harris Leah Roberts Emad Ibrahim Nissar Shaikh Anand Deshmukh Mazen A. Sid Ahmed Muna Al Maslamani Kyra Cottrell Ella Trembizki Lana Sundac Heidi H. Yu Jian Li Mark A. Schembri David M. Whiley David L. Paterson Scott A. Beatson Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
description	ABSTRACT Resistance to last-line polymyxins mediated by the plasmid-borne mobile colistin resistance gene (mcr-1) represents a new threat to global human health. Here we present the complete genome sequence of an mcr-1-positive multidrug-resistant Escherichia coli strain (MS8345). We show that MS8345 belongs to serotype O2:K1:H4, has a large 241,164-bp IncHI2 plasmid that carries 15 other antibiotic resistance genes (including the extended-spectrum β-lactamase blaCTX-M-1) and 3 putative multidrug efflux systems, and contains 14 chromosomally encoded antibiotic resistance genes. MS8345 also carries a large ColV-like virulence plasmid that has been associated with E. coli bacteremia. Whole-genome phylogeny revealed that MS8345 clusters within a discrete clade in the sequence type 95 (ST95) lineage, and MS8345 is very closely related to the highly virulent O45:K1:H4 clone associated with neonatal meningitis. Overall, the acquisition of a plasmid carrying resistance to colistin and multiple other antibiotics in this virulent E. coli lineage is concerning and might herald an era where the empirical treatment of ST95 infections becomes increasingly more difficult. IMPORTANCE Escherichia coli ST95 is a globally disseminated clone frequently associated with bloodstream infections and neonatal meningitis. However, the ST95 lineage is defined by low levels of drug resistance amongst clinical isolates, which normally provides for uncomplicated treatment options. Here, we provide the first detailed genomic analysis of an E. coli ST95 isolate that has both high virulence potential and resistance to multiple antibiotics. Using the genome, we predicted its virulence and antibiotic resistance mechanisms, which include resistance to last-line antibiotics mediated by the plasmid-borne mcr-1 gene. Finding an ST95 isolate resistant to nearly all antibiotics that also has a high virulence potential is of major clinical importance and underscores the need to monitor new and emerging trends in antibiotic resistance development in this important global lineage.
format	article
author	Brian M. Forde Hosam M. Zowawi Patrick N. A. Harris Leah Roberts Emad Ibrahim Nissar Shaikh Anand Deshmukh Mazen A. Sid Ahmed Muna Al Maslamani Kyra Cottrell Ella Trembizki Lana Sundac Heidi H. Yu Jian Li Mark A. Schembri David M. Whiley David L. Paterson Scott A. Beatson
author_facet	Brian M. Forde Hosam M. Zowawi Patrick N. A. Harris Leah Roberts Emad Ibrahim Nissar Shaikh Anand Deshmukh Mazen A. Sid Ahmed Muna Al Maslamani Kyra Cottrell Ella Trembizki Lana Sundac Heidi H. Yu Jian Li Mark A. Schembri David M. Whiley David L. Paterson Scott A. Beatson
author_sort	Brian M. Forde
title	Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
title_short	Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
title_full	Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
title_fullStr	Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
title_full_unstemmed	Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage
title_sort	discovery of <italic toggle="yes">mcr-1</italic>-mediated colistin resistance in a highly virulent <named-content content-type="genus-species">escherichia coli</named-content> lineage
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/774be1daa8cb4e5d8b8f79dd7eb81405
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Discovery of <italic toggle="yes">mcr-1</italic>-Mediated Colistin Resistance in a Highly Virulent <named-content content-type="genus-species">Escherichia coli</named-content> Lineage

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